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Platelet-rich plasma freeze-dried powder and preparation method and application thereof

A platelet-rich plasma and platelet technology, applied in the field of clinical medicine, can solve the problems of complicated operation, difficulty in applying human blood, undisclosed platelet yield, etc., and achieve the effect of high concentration and high recovery rate

Active Publication Date: 2017-03-15
FIVE DIMENSION BY INCOSC HEALTH MANAGEMENT JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of platelet enrichment in mouse blood is believed to yield concentrations up to 10 10 platelets, but this method does not disclose the yield of platelets, and the method is complicated in operation, and it is difficult to apply to the processing of human blood

Method used

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  • Platelet-rich plasma freeze-dried powder and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0142] Embodiment 1: extract platelet-rich plasma (PRP) and prepare its freeze-dried powder

[0143] (1) Collect the subject's whole blood into a container containing anticoagulant, which is a blood collection anticoagulant tube that can be centrifuged. The anticoagulant uses ethylenediaminetetraacetic acid.

[0144] (2) Fully mix the blood and anticoagulant to avoid coagulation. Extract 100 microliters to count platelets.

[0145] (3) Dispense the blood into centrifuge tubes (15 ml).

[0146] (4) Put the centrifuge tube into the centrifuge and centrifuge at 2400rpm for 4 minutes.

[0147] (5) The blood after centrifugation should be divided into three layers: the bottom layer is red blood cells, the top layer is platelet-containing plasma, and the middle layer is BuffyCoat, which contains platelets and white blood cells.

[0148] (6) Use a pipette to extract the uppermost layer of plasma and most of the buffy coat and transfer them to a new centrifuge tube, avoiding extr...

Embodiment 2

[0157] Embodiment 2: extract platelet-rich plasma (PRP) and prepare its freeze-dried powder

[0158] (1) Collect the subject's whole blood into a container containing anticoagulant, which is a blood collection anticoagulant tube that can be centrifuged. Disodium edetate was used as an anticoagulant.

[0159] (2) Fully mix the blood and anticoagulant to avoid coagulation. Extract 100 microliters to count platelets.

[0160] (3) Dispense the blood into centrifuge tubes (15 ml).

[0161] (4) Put the centrifuge tube into the centrifuge and centrifuge at 2400rpm for 4 minutes.

[0162] (5) The blood after centrifugation should be divided into three layers: the bottom layer is red blood cells, the top layer is platelet-containing plasma, and the middle layer is BuffyCoat, which contains platelets and white blood cells.

[0163] (6) Use a pipette to extract the uppermost layer of plasma and most of the buffy coat and transfer them to a new centrifuge tube, avoiding extraction o...

Embodiment 3

[0172] Embodiment 3: extract platelet-rich plasma (PRP) and prepare its freeze-dried powder

[0173] (1) Collect the subject's whole blood into a container containing anticoagulant, which is a blood collection anticoagulant tube that can be centrifuged. Dextrose citrate solution was used as anticoagulant.

[0174] (2) Fully mix the blood and anticoagulant to avoid coagulation. Extract 100 microliters to count platelets.

[0175] (3) Dispense the blood into centrifuge tubes (15 ml).

[0176] (4) Put the centrifuge tube into the centrifuge and centrifuge at 2400rpm for 4 minutes.

[0177] (5) The blood after centrifugation should be divided into three layers: the bottom layer is red blood cells, the top layer is platelet-containing plasma, and the middle layer is BuffyCoat, which contains platelets and white blood cells.

[0178] (6) Use a pipette to extract the uppermost layer of plasma and most of the buffy coat and transfer them to a new centrifuge tube, avoiding extrac...

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Abstract

The invention relates to platelet-rich plasma freeze-dried powder and a preparation method and application thereof, in particular to a method for extracting platelet-rich plasma from blood and preparing the free-dried powder of the platelet rich plasma. The method includes the steps of (a), placing whole blood into a container with anticoagulation agents, and evenly mixing the blood with the anticoagulation agents; (b), placing the blood in a centrifugal tube, and performing primary centrifugation to separate the blood into three layers; (c), extracting the upper layer and most of the middle layer, transferring the extracted blood into a new centrifugal tube, evenly mixing, and performing secondary centrifugation; (d), discarding the upper plasma of the centrifugal tube, enabling sedimentary blood platelets to be re-suspended by using the residual plasma to obtain the platelet-rich plasma; (e), adding freeze-drying excipients into the platelet-rich plasma, and freeze-drying the platelet-rich plasma to obtain the platelet-rich plasma freeze-dried powder. The plateletrich plasma freeze-dried powder is applicable to the fields such as the orthopaedics department, the stomatology department, dentofacial surgery, sport medicine and cosmetic medicine. The freeze-dried powder comprises the freeze-drying excipients and has an excellent technical effect.

Description

technical field [0001] The invention belongs to the technical field of clinical medicine, in particular to a method for preparing platelet-rich plasma, and more particularly to a method for obtaining platelet-rich plasma with one or more advantages such as high platelet concentration and high platelet recovery rate. Preparation. The present invention also relates to the platelet-rich plasma PRP prepared by the method of the present invention, and the use of the platelet-rich plasma PRP prepared by the method of the present invention in the preparation of health products. For example, said health products are used in orthopedics, stomatology, maxillofacial surgery, sports medicine and aesthetic medicine. The present invention also relates to a method for preparing freeze-dried powder from the platelet-rich plasma PRP prepared by the present invention and the prepared freeze-dried powder. Background technique [0002] Platelet-rich plasma, also commonly referred to as platel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K35/16A61K47/26A61K47/12A61K47/18
CPCA61K9/19A61K35/16A61K47/12A61K47/183A61K47/26
Inventor 李诣书尚聪梅俊周丹
Owner FIVE DIMENSION BY INCOSC HEALTH MANAGEMENT JIANGSU
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