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Method for preparing platelet rich plasma through one-step centrifugation method

A technology of platelet-rich plasma and platelet-poor plasma, which is applied in separation methods, chemical instruments and methods, liquid separation, etc., can solve the problems of high preparation cost of marketed equipment sets, complicated preparation process of manual secondary centrifugation, low platelet-rich plasma concentration or Purity is not high, to reduce the operation time and contamination risk, reduce the length of direct exposure, increase the complexity of the effect

Inactive Publication Date: 2014-01-15
广东威尔科技推广有限公司
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention overcomes the shortcomings of the current traditional manual method, such as low concentration or purity of platelet-rich plasma, the complicated preparation process of the manual secondary centrifugation method, and the high preparation cost of the marketed equipment package, and provides a method for clinical research that can prepare The technology to obtain platelet-rich plasma with high concentration and purity, the preparation process is simple and low cost

Method used

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  • Method for preparing platelet rich plasma through one-step centrifugation method
  • Method for preparing platelet rich plasma through one-step centrifugation method
  • Method for preparing platelet rich plasma through one-step centrifugation method

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Embodiment 1

[0025] S1. Materials and methods

[0026] Main equipment and reagents: eppendorf 5702 centrifuge, centrifuge tube, EDTA blood collection tube, syringe, heparin sodium, automatic blood cell analyzer.

[0027] S2. Experimental subjects: First, select patients according to the following 6 points:

[0028] 1. Patients who intend to voluntarily receive platelet-rich plasma injection therapy.

[0029]2. The blood cell analysis results of the patient's venous blood were within the normal range of the three lines: the platelet count was 100-300×10 9 / L, hemoglobin for women is between 135-150g / L, for men is between 140-155g / L, white blood cell count is 4-10×10 12 / L between.

[0030] 3. Erythrocyte sedimentation rate and C-reactive protein were within the normal range.

[0031] 4. Those who have no symptoms of infection in the whole body and treatment area.

[0032] 5. The basic condition is good, without hyperlipidemia, diabetes and other diseases outside the blood syste...

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Abstract

The invention belongs to the technical field of preparation of platelet rich plasma and in particular discloses a method for preparing PRP (Platelet Rich Plasma) through a one-step centrifugation method. According to the method, only a high-speed automatic centrifuge is utilized, the one-step centrifugation method is adopted, only one-step liquor relief (which is used for absorbing plasma on a boundary layer) is required in the whole process, and the purposes of convenience, rapidness and low pollution risk are achieved. Moreover, according to the platelet rich plasma prepared by the method, a mean value of platelet cell concentrations reaches 1029.875*10<9> / L, the mean corpuscular hemoglobin in the PRP is 20.26 g / L, and the clinical requirements can be well met.

Description

technical field [0001] The invention relates to a method for preparing platelet-rich plasma, in particular to a method for preparing platelet-rich plasma by a single centrifugation method. Background technique [0002] Platelet-rich plasma (platelet-rich plasma, PRP) is the plasma containing high volume fraction of platelets obtained by centrifuging the whole blood of human or animal itself. Since DAVID R. KNIGHTON and others discovered that platelet cells can promote vascular proliferation and collagen synthesis in the 1980s, people have devoted themselves to clinical application of platelet-rich plasma (PRP), eager to solve the problem of repairing the damage of organs and tissues with low repair ability. . However, due to the difficulty in preparing PRP at that time, its clinical promotion was limited. [0003] After decades of research, several more suitable methods for preparing platelet-rich plasma have been explored: manual methods (one-time centrifugation, two-tim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D17/038
Inventor 郑秋坚林子洪沈梓维
Owner 广东威尔科技推广有限公司
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