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Human skin filler for injection and its prepn process

A technology of human skin and fillers, applied in prosthetics, medical science, surgery, etc., can solve the problems of slow, generally 4-6 months, weak cell proliferation and survival, low collagen content, etc., to achieve Reduce infection of zoonotic diseases, improve skin elasticity and radiance, and improve the effect of skin matrix

Inactive Publication Date: 2007-12-19
北京赛尔泰和生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the biomaterials used in this technology are taken from the patient's own body, there is no rejection and the effect is long-lasting. However, the disadvantage of this technology is that the collagen content in the injection that can immediately perform the filling and repairing functions of human soft tissues is low, which leads to injections in the human body. It takes 4-6 months to see results after scars and wrinkles; in addition, using animal serum as a component of the culture solution may also have safety issues
[0005] In 2006, we invented a new filling material composed of autologous fibroblast precursor cells and hyaluronic acid, which effectively improved the slow effect of pure fibroblast therapy, but the main function of hyaluronic acid is water retention , have a weaker effect on cell expansion and survival

Method used

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  • Human skin filler for injection and its prepn process
  • Human skin filler for injection and its prepn process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0046] Embodiment: the preparation of human soft tissue filler

[0047] 1. Extraction of autologous healthy skin samples

[0048] Disinfect the back of the ear with 70% alcohol, and then perform local topical anesthesia with 10% lidocaine and 1 / 100,000 epinephrine. Use a skin picker or a scalpel and surgical scissors to remove the 4 square millimeters of epidermis and dermis tissue behind the ear of the recipient, put them in a tissue preservation solution (DMEM cell preservation solution, purchased from Hyclone, USA), and make the wound site One stitch, covered with a Band-Aid.

[0049] 2. Preparation of cell suspension

[0050] 1. Pre-treatment of skin tissue culture: place the skin tissue block in a petri dish to clean the epithelial part, then remove the subcutaneous tissue and fat, cut the skin tissue into 0.1-0.5 mm2 skin fragments, and finally spread the skin fragments on the The bottom surface of the Petri dish.

[0051] 2. Primary cell culture: Add DMEM and 3% fet...

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Abstract

The human skin filler for injection consists of cell components, blood plasma with rich thrombocyte, blood coagulation factor and calcium ion. Each milliliter of the filler for injection contains cell components in (10-50)x10<6>, thrombocyte in (100-700)x10<6>, blood coagulation factor in 1-10 U, and calcium ion in 10-40 mmol. The cell components are at least one of corium stem fibroblast, fibroblast precursor and fibroblast obtained through acquiring the skin from the patient, extracorporeal no-serum culturing and amplifying, and collecting; the thrombocyte is extracted from the autoblood of the patient; and the blood coagulation factor is autogenous or extraneous. The present invention also provides preparation process of human soft tissue filler for injection. The present invention has the advantages of quick obvious filling and repairing effect, last curative effect, high safety, etc.

Description

technical field [0001] The invention relates to a tissue filler and a preparation method thereof, more specifically, a human skin filler for injection and a preparation method thereof. Background technique [0002] Human soft tissue augmentation is the main treatment method for repairing soft tissue after injury. In the nineteenth century, paraffin and silicone were used for filling. After the 1980s, gelatin powder materials appeared again, but they all had problems such as uncertain curative effect and large toxic and side effects. [0003] After 1997, Isolagen used autologous fibroblasts as mass tissue filling materials, which had a certain curative effect, but there were problems such as slow effect and insignificant curative effect. [0004] Chinese patent 03155833.X discloses an injection for removing wrinkles and scars. It uses autologous skin, which is isolated, cultured and amplified in vitro, and is made into an injection in glucose solution to treat scars and rem...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L31/00A61L27/60A61F2/02
Inventor 韩斌
Owner 北京赛尔泰和生物医药科技有限公司
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