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Injection for treating skin defects and preparation method thereof

A technology for skin defects and injections, applied in skin diseases, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of safety, slow effect, low collagen content, etc., to improve skin elasticity and luster, Enhance the thickness of the dermis and improve the effect of the skin matrix

Inactive Publication Date: 2009-10-07
北京赛尔泰和生物医药科技有限公司
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AI Technical Summary

Problems solved by technology

Since the biomaterials used in this technology are taken from the patient's own body, there is no rejection and the effect is long-lasting. However, the disadvantage of this technology is that the collagen content in the injection that can immediately perform the filling and repairing functions of human soft tissues is low, which leads to injections in the human body. It takes 4-6 months to see results after scars and wrinkles; in addition, using animal serum as a component of the culture solution may also have safety issues
[0005] In 2006, we invented a new filling material composed of autologous fibroblast precursor cells and hyaluronic acid, which effectively improved the slow effect of pure fibroblast therapy, but the main function of hyaluronic acid is water retention , have a weaker effect on cell expansion and survival

Method used

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  • Injection for treating skin defects and preparation method thereof
  • Injection for treating skin defects and preparation method thereof
  • Injection for treating skin defects and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0041] Embodiment: the preparation of human soft tissue filler

[0042] 1. Extraction of autologous healthy skin samples

[0043]Use 70% alcohol to sterilize the skin of aborted fetuses or the resected foreskin specimens of neonates, put them into tissue preservation solution (DMEM cell preservation solution, purchased from Hyclone Company of the United States), and send them to the laboratory for isolation and culture.

[0044] 2. Preparation of cell suspension

[0045] 1. Pre-treatment of skin tissue culture: place the skin tissue block in a petri dish to clean the epithelial part, then remove the subcutaneous tissue and fat, cut the skin tissue into 0.1-0.5 mm2 skin fragments, and finally spread the skin fragments on the The bottom surface of the Petri dish.

[0046] 2. Primary cell culture: Add DMEM and 3% fetal bovine serum to the petri dish that has been tiled with skin fragments, and put it into Forma CO 2 Model 3131 incubator at 37 °C, 5% CO 2 The above culture me...

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Abstract

The invention relates to injection for treating skin defects which takes cells as main component, and the content of cell component in every milliliter of filler used for injection is 10-50 million; the cell component is of at least one type of dermal fibroblast stem cells, fibroblast precursor cells and fibroblasts which are obtained by collecting skin of aborted fetus and excised foreskin of newborn after in vitro culture and proliferation through low serum or serum-free. The invention also provides a preparation method of the injection for treating skin defects. The invention has the advantages of immediate and obvious filling and repairing effects after injection, lasting curative effect, and simple and convenient treating procedures.

Description

technical field [0001] The invention relates to a tissue filler and a preparation method thereof, more specifically, an injection for treating skin defects and a preparation method thereof. Background technique [0002] Human soft tissue augmentation is the main treatment method for repairing soft tissue after injury. In the nineteenth century, paraffin and silicone were used for filling. After the 1980s, gelatin powder materials appeared again, but they all had problems such as uncertain curative effect and large toxic and side effects. [0003] After 1997, Isolagen used autologous fibroblasts as mass tissue filling materials, which had a certain curative effect, but there were problems such as slow effect and insignificant curative effect. [0004] Chinese patent 03155833.X discloses an injection for removing wrinkles and scars. It uses autologous skin, which is isolated, cultured and amplified in vitro, and is made into an injection in glucose solution to treat scars an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/36A61K9/10A61P17/02
Inventor 韩斌
Owner 北京赛尔泰和生物医药科技有限公司
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