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Elastic gel support material for bone tissue engineering and preparation method of material

A technology of bone tissue engineering and elastic gel, applied in medical science, prosthesis, etc., can solve the problems of loss of mechanical properties, easy dissolution, poor cell adhesion, etc., and achieve excellent biological functions and mechanical properties

Inactive Publication Date: 2014-07-16
NINGBO UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because divalent ions are easily exchanged with ions in the surrounding medium environment of the gel, the gel is easily dissolved in the body fluid environment and loses its mechanical properties.
In addition, due to its strong hydrophilicity, alginate has poor adhesion to proteins, and the lack of specific adsorption sites for cells limits the application of this gel.
[0007] At present, the existing double network gel system has good mechanical properties, but its cell adhesion is poor, it cannot grow on it, and it cannot be used as a tissue engineering material

Method used

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  • Elastic gel support material for bone tissue engineering and preparation method of material
  • Elastic gel support material for bone tissue engineering and preparation method of material
  • Elastic gel support material for bone tissue engineering and preparation method of material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Step (1). Add 1.09 g of gel N1 reactive monomer (c), 0.54 g of gel N1 reactive monomer (h), 0.024 g of N,N-methylenebisacrylamide cross-linked into a 15 ml plastic tube. The joint agent and tetramethylethylenediamine accelerator were dissolved in 6.5 ml of deionized water, and nitrogen gas was introduced for 30 minutes to remove the oxygen in the system. Shake well and react at 25°C for 12h to obtain gel N1.

[0049] Step (2). Take the gel N1 out of the plastic tube and cut it into a 1-3 cm columnar gel block with a knife. Prepare a 200 mL gel N2 reaction solution of acrylamide (28.4 g), α-ketoglutaric acid (0.58 g), and N,N-methylenebisacrylamide (0.062 g). Put the gel N1 gel block into the gel N2 reaction solution, seal it with aluminum foil, and shake it gently for 48 hours. Then, the fully soaked gel block was taken out and irradiated under an ultraviolet lamp at 25°C for 6 hours, and the double network gel was obtained by photo-initiated radical polymerization. ...

Embodiment 2

[0052] Step (1). Add 1.09 g of gel N1 reactive monomer (c), 0.54 g of gel N1 reactive monomer (h), 0.024 g of N,N-methylenebisacrylamide cross-linked into a 15 ml plastic tube. The joint agent and tetramethylethylenediamine accelerator were dissolved in 6.5 ml of deionized water, and nitrogen gas was introduced for 30 minutes to remove the oxygen in the system. Shake well and react at 25°C for 12h to obtain gel N1.

[0053] Step (2). Take the gel N1 out of the plastic tube and cut it into a 1-3 cm columnar gel block with a knife. Prepare a 200 mL gel N2 reaction solution of acrylamide (56.8 g), α-ketoglutaric acid (1.16 g), and N,N-methylenebisacrylamide (0.124 g). Put the gel N1 gel block into the gel N2 reaction solution, seal it with aluminum foil, and shake it gently for 48 hours. Then, the fully soaked gel block was taken out and irradiated under a UV lamp at 25°C for 8 hours, and the double network gel was obtained by photo-initiated radical polymerization.

Embodiment 3

[0055] The double network gel obtained in Example 2 was cut into small pieces of 5 mm * 5 mm * 2 mm, washed three times with deionized water, frozen at -20 ° C, and vacuum dried, and then soaked in 70% alcohol aqueous solution After 2 hours, wash with PBS three times in a sterile environment, and then soak in PBS overnight.

[0056] Adipose-derived mesenchymal stem cells were planted on double network gel, and DMEM growth medium containing 10% fetal bovine serum was added, and cultured for 3 days at 37°C under 5% carbon dioxide environment, and stained with DAPI dye. A large number of adipose stem cells adhered and grew on the surface of the gel, indicating that the gel has excellent biocompatibility. When the fullness increased to about 80%, the osteoinductive medium (StemPro, GIBCO) was used for culturing for 21 days at 37°C and 5% carbon dioxide. The double-network gel scaffold was stained with alizarin, and the calcification on the surface of the gel was observed under a ...

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Abstract

The invention discloses an elastic gel support material for bone tissue engineering and a preparation method of the material. The material consists of a gel N1 and a gel N2, wherein the molar content of the gel N1 is 5-50% and the molar content of the gel N2 is 50-95%. The preparation method comprises the following steps: fully dissolving a gel N1 reaction monomer, a gel N1 crosslinking agent and a gel N1 accelerant in deionized water to obtain a gel N1 synthesized solution; introducing N2 to remove oxygen in the solution, and adding a free radical initiator to initiate polymerization of the free radical to obtain the gel N1; fully dissolving a gel N2 reaction monomer, a gel N2 crosslinking agent and a photoinitiator in deionized water to obtain a gel N2 synthesized solution; and adding the gel N1 into the gel N2 synthesized solution to be fully immersed, and then irradiating and photo-initiating polymerization of the free radical under an ultraviolet lamp to obtain a double-network gel. The component of gel in the material is cytoactive, can adapt in vivo dynamic environment and can be applied to bone tissue repair materials.

Description

technical field [0001] The invention relates to a hydrogel tissue engineering material, in particular to an elastic gel support material for bone tissue engineering, which can be used as a carrier for cartilage repair and drug controlled release systems. Background technique [0002] Cartilage tissue regeneration ability is poor, cartilage defect repair is a difficult problem faced by clinical medicine. At present, it is mainly repaired by autologous transplantation or allogeneic transplantation. Autologous transplantation has the disadvantages of few donor sources, and it is easy to cause deformity and infection of the donor site after surgery. Allogeneic cartilage transplantation also has the problems of insufficient donor sources and immune diseases, both of which are difficult to achieve satisfactory results. The use of tissue engineering methods to prepare biocompatible cell scaffolds and cell transplantation to repair tissue is a new way to solve this problem. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/52A61L27/38C08F220/34C08F222/22C08F220/06C08F220/28C08F220/54C08F230/02C08F220/38C08F220/56C08F222/38C08F222/14C08F290/06C08F2/48
Inventor 蒋志强杨建邵双喜顾群
Owner NINGBO UNIVERSITY OF TECHNOLOGY
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