The invention provides a micro-pipeline-based liquid droplet generation method which comprises the following steps: firstly, providing a micro-pipeline with an opening in one end, wherein the micro-pipeline is full of a first liquid; providing an opened container with a second liquid; positioning the micro-pipeline above the liquid level of the opened container; enabling the micro-pipeline to move downwards from the part above the liquid level of the opened container, thereby enabling the opening of the micro-pipeline to contact and be fed into the second liquid, and positioning the first liquid at the opening of the micro-pipeline; subsequently enabling the micro-pipeline of which the opening enters the second liquid to move upwards to leave off the liquid level of the opened container, thereby dissociating the opening of the micro-pipeline from the second liquid, dissociating the first liquid at the opening of the micro-pipeline from the micro-pipeline, and forming liquid droplets in the second liquid, wherein the first liquid and the second liquid are two liquids which are not compatible with each other or two liquids with interface reaction. The liquid droplet generation method is simple and convenient, liquid droplets with controllable sizes can be formed, and the precision and the efficiency in generating large-scale liquid droplets are improved.