Scaffold containing platelets and hyaluronic acid and preparation method of scaffold

A technology of hyaluronic acid and platelets, applied in the field of biomedical materials, can solve the problems of unsatisfactory effects of biological scaffold materials, achieve good industrial application prospects, prolong shelf life, and high production efficiency

Inactive Publication Date: 2015-05-06
SHENZHEN CHINA CAPTICAL BIOMEDICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a biological scaffold, which has a porous structure, can maintain the slow release of growth factors, and can maintain platelet activity after long-term low-temperature storage, aiming to solve the unsatisfactory effect of biological scaffold materials in the prior art The problem

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  • Scaffold containing platelets and hyaluronic acid and preparation method of scaffold
  • Scaffold containing platelets and hyaluronic acid and preparation method of scaffold
  • Scaffold containing platelets and hyaluronic acid and preparation method of scaffold

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preparation example Construction

[0029] The embodiment of the present invention also provides a method for preparing a biological scaffold, the method comprising the following steps:

[0030] S01 Preparation of PRP: Obtain venous blood and prepare platelet-rich plasma PRP by secondary centrifugation, wherein the secondary centrifugation is as described above;

[0031] S02 Platelet activation: add calcium chloride solution or calcium gluconate solution to the PRP obtained in step S01, so that the final concentration of calcium ions in the resulting mixture is 0.1%-0.5%, and then place it at 30°C-37°C for 5-20min;

[0032] S03 Mix the above platelet-activated PRP with 0.5-2% hyaluronic acid solution at a volume ratio of 1:1-1:4.

[0033] The bioscaffold prepared by the embodiment of the present invention can maintain the activity of platelets, so that it can slowly release growth factors, and at the same time exert the biocompatibility of hyaluronic acid, promote the sustained release of drugs and prevent posto...

Embodiment 1

[0042] Example 1 Preparation method of PRP / HA composite biological scaffold

[0043] S01 Obtain venous blood and prepare platelet-rich plasma PRP by secondary centrifugation. The specific operation steps of the secondary centrifugation can be found in: Jin Zhe et al., "Comparison of centrifugation conditions for the preparation of platelet-rich plasma by secondary centrifugation", Journal of China Medical University , Volume 41, Issue 3, March 2012.

[0044] S02 Add calcium chloride solution to the platelet-rich plasma obtained in step S01 so that the final concentration of calcium chloride in the obtained plasma mixture is 0.5%, mix well, and then place it at 37° C. for 5 minutes to fully proceed the activation reaction.

[0045] S03 Mix the PRP containing calcium chloride-activated platelets obtained in step S02 with a 0.5% hyaluronic acid solution at a ratio of 1:4.

[0046] S04 Let the mixture obtained in step S03 stand at room temperature for 3 hours, so that the hyaluro...

Embodiment 2

[0048] Example 2 Preparation method of PRP / HA composite bioscaffold

[0049] S01 obtains venous blood, and prepares platelet-rich plasma PRP by a second centrifugation method, wherein the specific operation steps of the second centrifugation method are the same as in Example 1.

[0050] S02 Add calcium gluconate solution to the platelet-rich plasma obtained in step S01, so that the final concentration of calcium gluconate in the obtained plasma mixture is 0.1%, mix well, and then place it at 30°C for 20 minutes, so that calcium ions can fully activate the platelets .

[0051] S03 Mix the PRP containing activated platelets obtained in step S02 with a 2% hyaluronic acid solution at a ratio of 1:1.

[0052] S04 Let the mixture obtained in step S03 stand at room temperature for 2 hours, so that the hyaluronic acid in the mixture is fully cross-linked and formed, and a platelet-containing PRP / hyaluronic acid hybrid gel is obtained.

[0053] S05 Freeze the gel obtained in step S04...

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Abstract

The invention is suitable for the technical field of biomedicine materials and provides a biological scaffold containing platelets and hyaluronic acid which are mixed uniformly, wherein the platelets are activated by calcium ions before being mixed with hyaluronic acid. The invention also provides a preparation method of the biological scaffold. The preparation method comprises the following steps: obtaining platelet-rich plasma (PRP); adding calcium ions into the PRP; and uniformly mixing the obtained PRP with hyaluronic acid. The biological scaffold provided by the invention has good biological compatibility and more pore structures and can ensure that the platelets slowly release growth factors.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a biological scaffold containing platelets and hyaluronic acid, a preparation method and an application thereof. Background technique [0002] Medical biomaterials have a wide range of applications in tissue damage repair and plastic surgery. The theoretical basis of its application is to simulate the physiological environment in vivo in vitro, make the normal tissue cells cultured and expanded in vitro adhere to the biocompatible and biodegradable bioscaffold material, and provide nutrients to the cells to make them expand. When cells and biological scaffolds form complexes, the complexes can be implanted into diseased parts of body tissues or organs. As the scaffold material gradually degrades in the body, the cells continue to proliferate and secrete extracellular matrix, thereby obtaining new tissue that is consistent with the corresponding tissue an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/56A61L27/36A61L27/52
Inventor 沈政郭小勇张文陈倩文
Owner SHENZHEN CHINA CAPTICAL BIOMEDICAL TECH CO LTD
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