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Extraction method of platelet rich plasma (PRP) and extracted PRP

A platelet-rich plasma and platelet technology, applied in the field of clinical medicine, can solve the problems of difficulty in applying human blood, undisclosed platelet yield, complicated operation, etc., and achieve the effects of high recovery rate, simple operation process and high concentration

Active Publication Date: 2012-10-31
FIVE DIMENSION BY INCOSC HEALTH MANAGEMENT JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of platelet enrichment in mouse blood is believed to yield concentrations up to 10 10 platelets, but this method does not disclose the yield of platelets, and the method is complicated in operation, and it is difficult to apply to the processing of human blood

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Extraction of platelet-rich plasma (PRP)

[0057] (1) Platelet concentration in whole blood before separation: 250×10 6 / ml, the volume of whole blood is 10ml. Total platelet count is 2.5 x 10 9 .

[0058] (2) Platelet concentration in plasma after the first centrifugation: 570×10 6 / ml (concentrated 2.28 times), the plasma volume is 4ml. The total platelet count was 2.28×10 9 . The recovery rate was 91.2%.

[0059] (3) Platelet concentration in plasma (PRP) after the second centrifugation: 1685×10 6 / ml (concentrated 6.74 times), the plasma volume is 1.2ml, and the platelet-rich plasma is obtained. The total platelet count was 2.02×10 9 . The final recovery rate was 80.8%.

Embodiment 2

[0060] Example 2: Extraction of platelet-rich plasma (PRP)

[0061] Disodium edetate was used as the anticoagulant, and the weight ratio of anticoagulant to whole blood was 1:8.

[0062] (1) Platelet concentration in whole blood before separation: 165×10 6 / ml, the volume of whole blood is 10ml. The total platelet count was 1.65 x 10 9 .

[0063] (2) Platelet concentration in plasma after the first centrifugation: 444×10 6 / ml (concentrated 2.69 times), the plasma volume is 3.5ml. The total platelet count was 1.55 x 10 9 . The recovery rate was 93.9%.

[0064] (3) Platelet concentration in plasma (PRP) after the second centrifugation: 1105×10 6 / ml (concentrated 6.70 times), the plasma volume is 1.2ml, and the platelet-rich plasma is obtained. The total platelet count was 1.33×10 9 . The final recovery rate was 80.6%.

Embodiment 3

[0065] Example 3: Extraction of platelet-rich plasma (PRP)

[0066] Glucose citrate was used as an anticoagulant.

[0067] (1) Platelet concentration in whole blood before separation: 210×10 6 / ml, the volume of whole blood is 10ml. The total platelet count was 2.1 x 10 9 .

[0068] (2) Platelet concentration in plasma after the first centrifugation: 542×10 6 / ml (concentrated 2.58 times), the plasma volume is 3.5ml. The total platelet count was 1.90 x 10 9 . The recovery rate was 90.5%.

[0069] (3) Platelet concentration in plasma (PRP) after the second centrifugation: 1370×10 6 / ml (concentrated 6.52 times), the plasma volume is 1.2ml, and the platelet-rich plasma is obtained. The total platelet count was 1.64×10 9 . The final recovery rate was 78.1%.

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Abstract

The invention relates to an extraction method of platelet rich plasma (PRP) and extracted PRP and particularly relates to a method for extracting PRP from blood. The method comprises the following steps of: (a) putting the collected whole blood in a container containing an anticoagulant, and fully and uniformly mixing the blood and the anticoagulant; (b) putting the blood mixed with the anticoagulant in a centrifuge tube, carrying out primary centrifugation, and enabling the blood to be divided into three layers; (c) extracting the uppermost layer and most of the middle layer, transferring the uppermost layer and most of the middle layer into a new centrifuge tube, mixing uniformly and carrying out secondary centrifugation; and (d) discarding the plasma at the upper layer of the centrifuge tube, and resuspending the precipitated plasma by utilizing the remaining plasma, thus obtaining the PRP. The method provided by the invention has the advantages that the operation process is simple, the yield is high and the concentration of platelet in rich plasma is high.

Description

technical field [0001] The invention belongs to the technical field of clinical medicine, and specifically relates to a method for extracting platelet-rich plasma. The platelet-rich plasma obtained under specific conditions by the method of the invention has at least one advantage of high platelet concentration and high platelet recovery rate. Background technique [0002] Platelet-rich plasma may be referred to as platelet-rich plasma (Platelet Rich Plasma, PRP) in the present invention, which is high-concentration platelet-containing plasma obtained by centrifuging blood. In addition to providing coagulation during hemostasis, platelets in the human body also contain many growth factors related to wound healing and bone regeneration. Such as platelet-derived growth factor (Platelet Derived Growth Factor, PDGF), transforming growth factor (Transforming Growth Factor, TGF), insulin-like growth factor (Insulin-like Growth Factor, IGF), epidermal growth factor (Epidermal Growt...

Claims

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Application Information

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IPC IPC(8): B01D21/26A61K35/14A61K35/19
Inventor 林卓衡陈俊峯
Owner FIVE DIMENSION BY INCOSC HEALTH MANAGEMENT JIANGSU
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