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Preparation method of water-soluble yellow fluorescent carbon quantum dots

A yellow fluorescence, carbon quantum dot technology, applied in biochemical equipment and methods, fluorescence/phosphorescence, chemical instruments and methods, etc., can solve the disadvantages of continuous and large-scale production of fluorescent carbon quantum dots, low carbon quantum yield, non-renewable energy and other issues, to achieve the effect of shortening the activation time, fluorescence stability, and rich carbon source

Active Publication Date: 2017-12-22
苏州诺维康生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method often requires strict experimental conditions and special energy sources, the cost is high, and the obtained carbon quantum yield is relatively low
Bottom-up synthesis method, that is, direct synthesis of fluorescent carbon quantum dots by pyrolysis or carbonization of suitable precursors, methods include: combustion method, hydrothermal carbonization method, ultrasonic method, etc., but these methods are often used Non-renewable energy and requires strict post-processing, so it is not conducive to continuous and large-scale production of fluorescent carbon quantum dots

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  • Preparation method of water-soluble yellow fluorescent carbon quantum dots
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  • Preparation method of water-soluble yellow fluorescent carbon quantum dots

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Embodiment 1

[0030] A preparation method of water-soluble yellow fluorescent carbon quantum dots, comprising the following steps:

[0031] S1. Mix pancreatin, ethylenediamine, and ultrapure water with a mass volume ratio of 1.5g:1mL:9mL to obtain a mixed solution, and the mixed solution is subjected to hydrothermal reaction to obtain a reaction solution; wherein, the mixed solution in step S1 Including 1.5g of trypsin, 1mL of ethylenediamine, and 9mL of ultrapure water. The hydrothermal reaction is as follows: put the mixed solution in a 25ml reactor and stir for 5 minutes. The reaction temperature is controlled at 170-190°C. The time is 9.5~10.5h, and the reaction liquid is obtained, wherein, the reaction liquid is dark brown;

[0032] S2. Take the reaction solution and go through centrifugation, filtration, dialysis, and evaporation to prepare water-soluble yellow fluorescent carbon quantum dots. The centrifugation time is 20 minutes. After centrifuging the reaction solution, take the su...

Embodiment 2

[0034] A preparation method of water-soluble yellow fluorescent carbon quantum dots, comprising the following steps:

[0035] S1. Mix pancreatin, ethylenediamine, and ultrapure water with a mass volume ratio of 1.5g:1mL:9mL to obtain a mixed solution, and the mixed solution is subjected to hydrothermal reaction to obtain a reaction solution; wherein, the mixed solution in step S1 Including 1.5g of trypsin, 1mL of ethylenediamine, and 9mL of ultrapure water, the hydrothermal reaction is as follows: put the mixed solution in a 25ml reactor and stir for 5min, control the reaction temperature at 180°C, and the reaction time is 10h, obtained reaction solution, wherein, reaction solution is dark brown;

[0036] S2. Take the reaction solution and go through centrifugation, filtration, dialysis, and evaporation to prepare water-soluble yellow fluorescent carbon quantum dots. The centrifugation time is 20 minutes. After centrifuging the reaction solution, take the supernatant and use m...

Embodiment 3

[0038] A preparation method of water-soluble yellow fluorescent carbon quantum dots, comprising the following steps:

[0039] S1. Mix pancreatin, ethylenediamine, and ultrapure water with a mass volume ratio of 1.5g:1mL:9mL to obtain a mixed solution, and the mixed solution is subjected to hydrothermal reaction to obtain a reaction solution; wherein, the mixed solution in step S1 Including 1.5g of trypsin, 1mL of ethylenediamine, and 9mL of ultrapure water, the hydrothermal reaction is as follows: put the mixed solution in a 25ml reactor and stir for 5min, control the reaction temperature at 190°C, and the reaction time is 10.5h, obtained reaction solution, wherein, reaction solution is dark brown;

[0040] S2. Take the reaction solution and go through centrifugation, filtration, dialysis, and evaporation to prepare water-soluble yellow fluorescent carbon quantum dots. The centrifugation time is 20 minutes. After centrifuging the reaction solution, take the supernatant and use...

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Abstract

The invention discloses a preparation method of water-soluble yellow fluorescent carbon quantum dots. The preparation method includes the steps: S1 uniformly mixing pancreatic enzymes, ethylenediamine and ultra-pure water to obtain mixed liquid, and performing hydrothermal reaction on the mixed liquid to prepare reaction liquid; S2 performing centrifugation, filtration, dialysis and evaporation to prepare the water-soluble yellow fluorescent carbon quantum dots. The mass volume ratio of the pancreatic enzymes, the ethylenediamine to the ultra-pure water is 1.5g: 1mL: 9mL. The quantum dots take pancreatic enzymes and the ethylenediamine as raw materials which are natural, free from toxicity, low in cost and easy to obtain, the preparation method is simple, rapid and efficient by a hydrothermal method, and the yield of the quantum dots is high.

Description

technical field [0001] The invention relates to the technical field of fluorescent material preparation, in particular to a method for preparing water-soluble yellow fluorescent carbon quantum dots. Background technique [0002] In recent years, fluorescent carbon nanomaterials have attracted extensive research by researchers because of their good optical properties and low cytotoxicity. It has good photostability and no toxic side ions, is suitable for long-term detection and tracing experiments, and has broad application prospects in biomarkers and analysis and detection. Carbon quantum dots (CQDs) exhibit excellent properties such as hydrophilicity, low cytotoxicity, chemical and photostability, etc., which have attracted extensive attention and applications in the fields of chemistry, biomedicine, sensing and optoelectronics. [0003] There are currently two main methods for preparing carbon quantum dots: top-down method and bottom-up method. The top-down synthesis met...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y30/00B82Y40/00C12N9/94G01N21/64B42D25/36
CPCB82Y20/00B82Y30/00B82Y40/00B42D25/36C09K11/65C12N9/94C12Y304/21004G01N21/64G01N21/6402G01N21/6428G01N2021/6417G01N2021/6439
Inventor 黄珊肖琦姚建东杨二利
Owner 苏州诺维康生物科技有限公司
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