Metabonomics-based research method for toxicity effect of jujube flower nectar on honeybee

Abstract

The invention belongs to the technical field of plant poison toxicity evaluation, and provides a metabonomics-based research method for a toxicity effect of jujube flower nectar on honeybees, aiming at the current situation that the toxicity effect of the jujube flower nectar on the honeybees cannot be explained in the existing research. The research method comprise the steps of: collecting honeybees with jujube flower diseases and taking healthy honeybees as a contrast; collecting hemolymph of the two groups of honeybees; combining a UPLC-Q-TOF MS technology with multi-dimensional statisticalanalysis to carry out full spectrum analysis on the sample to obtain primary mass spectrum data and secondary mass spectrum data; adopting xCMS software to carry out peak extraction and metabolite identification on the data, screening metabolite targets and metabolic profiles related to honeybee jujube flower diseases from metabolite, tracking and screening of pathogenicity of jujube flower nectar on honeybees under the guidance of metabolite target analysis and metabolic profile analysis, and illustrating a toxic action mechanism of the jujube flower nectar on honeybees. The research methodhas the advantages of being low in cost, easy to operate, rapid in detection, high in sensitivity, strong in selectivity, good in repeatability, comprehensive in obtained information and the like.

Description

technical field [0001] The invention belongs to the technical field of phytotoxicity evaluation, and in particular relates to a metabolomics-based research method on the toxic effect of jujube nectar on bees; an evaluation method based on molecular biology, by studying the effect of jujube nectar on the metabolic pathway of Italian bees Effects, to evaluate the toxic effects of jujube flower nectar on bees. Background technique [0002] Jujube flower disease, also known as jujube flower poisoning, often occurs in the jujube flower nectar flow period. After the bees collect jujube nectar, a large number of collecting bees die, and the bee colony declines. Jujube flower disease not only affects the production of honey and royal jelly during jujube flowering period, but also affects the pollination of jujube trees by bees. Regarding the etiology of jujube flower disease, it is generally believed that the climate is dry, the honey juice is thick, and the concentration of potass...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a metabolomics-based research method on jujube nectar-induced toxic effects on bees in response to the fact that existing research cannot explain the t...

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