A research method based on metabolomics for jujube flower nectar-induced toxic effects on bees

Abstract

The invention belongs to the technical field of toxicity evaluation of botanical poisons, aiming at the fact that existing research cannot explain the toxic effect of jujube nectar on bees, it provides a metabolomics-based research method for the toxic effect of jujube nectar on bees; collecting jujube nectar Diseased bees and healthy bees were used as controls, and the hemolymph of these two groups of bees was collected, and the full spectrum analysis of the samples was performed using UPLC‑Q‑TOF MS technology combined with multidimensional statistical analysis to obtain primary mass spectrometry and secondary mass spectrometry data, using XCMS software Peak extraction and metabolite identification were performed on the data, and metabolite targets and metabolic profiles related to bee jujube flower disease were screened from the metabolites. Guided by metabolite target analysis and metabolic profile analysis, the pathogenicity of jujube flower nectar to bees was carried out. The tracking and screening of jujube flower nectar to explain the toxicity mechanism of jujube nectar to bees. The invention has the advantages of low cost, easy operation, rapid detection, high sensitivity, strong selectivity, good repeatability, comprehensive information acquisition and the like.

Description

technical field [0001] The invention belongs to the technical field of phytotoxicity evaluation, and in particular relates to a metabolomics-based research method on the toxic effect of jujube nectar on bees; an evaluation method based on molecular biology, by studying the effect of jujube nectar on the metabolic pathway of Italian bees Effects, to evaluate the toxic effects of jujube flower nectar on bees. Background technique [0002] Jujube flower disease, also known as jujube flower poisoning, often occurs in the jujube flower nectar flow period. After the bees collect jujube nectar, a large number of collecting bees die, and the bee colony declines. Jujube flower disease not only affects the production of honey and royal jelly during jujube flowering period, but also affects the pollination of jujube trees by bees. Regarding the etiology of jujube flower disease, it is generally believed that the climate is dry, the honey juice is thick, and the concentration of potass...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a metabolomics-based research method on jujube nectar-induced toxic effects on bees in response to the fact that existing research cannot explain the toxic effects of jujube nectar on bees; metabolomics-based differential metabolic pathways , to excavate the mechanism of bee jujube disease, and provide a basis for the prevention and treatment of bee jujube disease

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