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A nematode preparation for controlling flower slugs and its preparation method and application

A preparation and a technology for slugs are applied in the field of nematode preparations and preparations for preventing and controlling flower slugs, can solve the problems of poor use effect and short duration of effect of parasitic nematodes, and can delay the generation of pest resistance, improve quick-acting properties, and protect the environment Effect

Active Publication Date: 2022-04-19
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the objectives of the present invention is to provide a nematode preparation for preventing and controlling flower slugs, which solves the problems of poor field use effect and short duration of parasitic nematodes in the prior art

Method used

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  • A nematode preparation for controlling flower slugs and its preparation method and application
  • A nematode preparation for controlling flower slugs and its preparation method and application
  • A nematode preparation for controlling flower slugs and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0038] This example provides the composition content table and preparation method of the nematode preparation of the present invention, see Table 1 for details.

[0039] Table 1

[0040]

[0041] Adopt the raw material in table 1 to prepare the method for the preparation A of nematode preparation of the present invention as follows:

[0042] Step 1. Expansion and cultivation of parasitic small rod nematodes:

[0043] The parasitic nematodes are infected and multiplied by the larvae of slugs; the reproduced third-instar infestation larvae (IJs) of the parasitic nematodes are collected in distilled water by the White trap method, and stored in a refrigerator at 4°C for 2 weeks before use ;

[0044] Step 2. Take each raw material component in proportion:

[0045] Concentrate the suspension of the parasitic C. nematodes collected in step 1 by static sedimentation, add L-dopa, add distilled water to make up to 1000 mL, mix well at room temperature, store in a refrigerator at ...

Embodiment 2

[0049] This example provides the ingredient content table and preparation method of the nematode preparation of the present invention, wherein both preparation A and preparation B are calculated as 1 L of preparation, see Table 2 for details.

[0050] Table 2

[0051]

[0052] Adopt the raw material in table 2 to prepare the method for the preparation A of nematode preparation of the present invention as follows:

[0053] Step 1. Expansion and cultivation of parasitic small rod nematodes:

[0054] The parasitic nematodes are infected and multiplied by the larvae of slugs; the reproduced third-instar infestation larvae (IJs) of the parasitic nematodes are collected in distilled water by the White trap method, and stored in a refrigerator at 4°C for 2 weeks before use ;

[0055] Step 2. Weigh the food-attractant base, humectant, preservative, and symbiotic bacteria into the same container in proportion, add distilled water to make it up to 900mL, mix well, put it into a tri...

Embodiment 3

[0060] This example provides the application of the preparation of the present invention to control flower slugs.

[0061] Test method: The control test was carried out at the Gladiolus gandavensis Vaniot Houtt breeding base in Longquanyi District, Chengdu in June 2020. A total of 6 treatments were set up, with 3 replicates for each treatment, a total of 18 plots, each plot 50m 2 (10m*5m), plots were distributed in random blocks, and 100 larvae of Phiolomycus bilineatus (body length about 20 mm, uniform in size, healthy) were evenly placed in each plot.

[0062] One day after placing the slugs, the application of the nematode preparation of the present invention was started. The application method is as follows: preparation B is sprayed on the buds and leaves of flowers in the afternoon, and 10ml is evenly sprayed on each flower; preparation A is applied on the rhizosphere soil surface of flowers in the evening of the same day, and 1.5g is evenly sprayed per square meter.

[...

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Abstract

The invention discloses a nematode preparation for preventing and controlling flower slugs, a preparation method and application thereof, and belongs to the technical field of biological pesticides. The nematode preparation of the present invention includes preparation A and preparation B containing different active ingredients, the active ingredient of preparation A includes L-dopa, a substrate inhibitor of parasitic rod nematode and polyphenol oxidase; the active ingredient of preparation B includes polyphenol oxidase Quercetin, a structural inhibitor of phenoloxidase. When applying the nematode preparation of the present invention to prevent and control flower slugs, preparation A is applied to the rhizosphere soil surface of flowers in the evening; preparation B is sprayed on the buds and leaves of flowers in the afternoon of the same day. In the present invention, for the first time, the slug bio-control factor parasitic rod nematode and the two functional inhibitors of slug polyphenol oxidase are used in combination, and the field quick-acting performance of the parasitic rod nematode is greatly improved by using the slug feeding attraction, and through the preparation A and formulation B selected different application locations, application times and methods, which accelerated nematode infestation and lethal slugs.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a nematode preparation for preventing and controlling flower slugs, a preparation method and application thereof. Background technique [0002] The slug Agriolimax agrestis Linnaeus is a collective name for gastropods, stalks, and slugs. In some areas of southern China, it is called gnat, which is a kind of mollusk. Slugs like to eat young plant tissues, and overwinter as adults or larvae under the wet soil at the roots of crops. From May to July, a large number of activities in the field cause damage. In summer, the temperature rises and the activities weaken. After the autumn climate is cool, the activities will cause damage again. Slugs are sensitive to light and die within 2-3 hours of strong light. Therefore, they are nocturnal. They start to mobilize in the evening, reach a peak at 10-11 pm, and sneak into the soil or hidden places one after anot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/12A01N43/16A01N37/44A01N25/02A01P7/04
CPCA01N63/12A01N43/16A01N37/44A01N25/02A01N25/006
Inventor 李星月邵宝林易军陈诚符慧娟李其勇张鸿向运佳陈友华李旭
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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