80 results about "Eriocheir japonica sinensis" patented technology

A composite feed for Eriocheir japonica sinensis is prepared from whole fish powder, bean cake, peanut cake, cotton seed cake, rapeseed cake, high-gluten flour, rice husk, corn embryo dregs, distilled corn generated by preparing alcohol, soybean phosphatide oil, lindly eupatorium herb additive, active palygorskite clay, calcium hydrogen phosphate, and exuviating agent through mixing, pulverizing, stirring, steaming, granulating, ageing, cooling and packing.

The invention discloses a Yangtze river system eriocheir sinensis compound feed, which is prepared from the following materials: fish meal, bean pulp, cottonseed meal, rapeseed meal, peanut meal, corn germ meal, cuttlefish paste, shrimp meal, soybean phosphatide powder, soya-bean oil, fish oil, lard oil, monocalcium phosphate, high protein flour, shell pigment, trace elements for crab, complex vitamins for crab, choline chloride, a binder, zeolite powder, bacillus licheniformis premix, and astragalus polysaccharide premix. The preparation method of the Yangtze river system eriocheir sinensis compound feed comprises the steps of: mixing materials twice, evenly mixing, pelletizing, keeping warm, cooling, sieving, packaging and the like. The Yangtze river system eriocheir sinensis compound feed can enhance the disease resistance of the eriocheir sinensis, quickens the growth speed of the eriocheir sinensis, enriches crab roe and crab cream, improves the nutritive value of the eriocheir sinensis, increases production and improves productivity for a farmer. The digestion and absorption efficiency of the eriocheir sinensis on the materials can be effectively strengthened, and the stability of particles in water is strong.

The invention discloses a gene engineering preparation and mass spectrometry identification method for eriocheir sinensis crustacean hyperglycemic hormone (Ers-CHH). Energy supplies of various tissues and organs are maintained by CHH through regulating a blood sugar level of a crustacean, and researches on family nerve polypeptide hormones can provide helps for revelation of crustacean metabolism, growth and reproduction regulation mechanisms. In previous studies, an eriocheir sinensis crustacean hyperglycemic hormone gene (Ers-CHH, GenBank registration number: JX485664) is cloned. According to the present invention, a gene engineering method is adopted to successfully obtain recombinant Ers-CHH, wherein a molecular weight is about 9.4 kD, a yield after purification renaturation is 0.4 g / L culture medium, and mass spectrometry identification results verify correctness of the recombinant protein. Due to low CHH content in the crustacean, direct purification enrichment on the CHH is virtually impossible, and a large number of the rare hormone proteins are rapidly obtained by using the gene engineering method in the present invention so as to establish a foundation for subsequent function regulation mechanism research development and application in aquaculture production practice.

An in vitro incubation and culture technique for Eriocheir japonica sinensis includes such steps as providing the living costegite crab, shearing out costegopod, hanging it from supporting frame, aerating while incubating in the water at 16-25 deg.C with salinity of 30-34, and culturing the larvae while controlling water quality.

The invention relates to the field of water purification, in particular to a method for managing pool eutrophication by planting rice. The method for managing pool eutrophication by planting the rice includes the following steps: a pool is aired and cleaned, and pests in the pool are killed; organic fertilizers for fermentation treatment are applied in middle of March; food organisms are started to be cultivated at the beginning of April; the food organisms are placed into the pool; reed rice seedlings are started to be cultivated at the beginning of May; rice seedling transplanting is started to be carried out in the middle of May; urea is applied after rice seedling transplanting is carried out; large-specification juvenile eriocheir sinensis, odontobutis obscura fries and an appropriate number of aristichthysnobilis fishes are input in the middle of June; compound fertilizers are applied at the beginning of July; harvesting is started to be carried out in the middle of November. By means of the method, rice is planted in the pool, the eriocheir sinensis and the odontobutis obscura which are high in economical value are bred in a mixed mode, aquatic products which are high in quality and high in economic value are produced while the water is purified, the price is higher the price of river crabs in the pool, ecological rice is achieved, and the pool cultivation comprehensive benefit is further improved.

The invention discloses an Eriocheir sinensis EsSXL gene, an amplification primer group thereof and an amplification method. A full-length cDNA (complementary Deoxyribose Nucleic Acid) sequence of the EsSXL gene is cloned from an Eriocheir sinensis testis tissue by using the primer group provided by the invention, and the EsSXL gene comprises two variable spliceosomes EsSXL1 and EsSXL2, and lays an important foundation for the research of the important function of the function of the Eriocheir sinensis EsSXL gene in a sex differentiation process of the Eriocheir sinensis EsSXL gene.

The invention discloses an SNP locus significantly relevant to the precocious puberty trait of eriocheir sinensis and the application. The SNP locus is positioned in a Vg gene 5'-flanking region-437bpposition of the eriocheir sinensis, and nucleotide variation information is SNP g.-437T) G. PCR primer used for detecting the SNP locus is SEQ ID NO.1 and SEQ ID NO.2, and restriction enzyme is MboI.The SNP locus has significant correlation with precocious puberty of the eriocheir sinensis, and the risk of precocious puberty trait of GG gene eriocheir sinensis individual is obviously smaller than that of precocious puberty trait of TT gene individual. The SNP locus and the PCR primer used for genetic typing are applied to the predication of the risk of precocious puberty of the eriocheir sinensis and / or the variety breeding of the eriocheir sinensis.

The invention discloses a breeding medium used for a juvenile shrimp and crab pool and a preparation method of the breeding medium.The breeding medium is prepared from, by mass, 10-30 parts of cow dung, 10-30 parts of sheep manure, 10-20 parts of distillers' grains, 5-15 parts of straw, 5-15 parts of corn flour, 5-15 parts of bean pulp, 1-6 parts of molasses, 1-7 parts of vegetable cakes and 2-6 parts of livestock excrement zymophyte.When the breeding medium is used for the breeding processes of freshwater shrimps, eriocheir sinensis and the like, a large number of fresh and good-quality aquatic organisms of water earthworms, chironomidae larvae, plankton and the like can be easily bred, good-quality bait is provided for growth of juvenile crabs, and therefore input of artificial bait is reduced, and the input cost is reduced; meanwhile, by means of the breeding medium, the purified water oxygen dissolving degree is easily increased, the water body environment is adjusted, a good ecological environment is provided for growth of shrimps and crabs, and the survival rate of shrimps and crabs is greatly increased; meanwhile, by means of the breeding medium, the water body fertility can be effectively increased, and growth of aquatic plant root systems is promoted.

The invention discloses a molecular specific primer and a method for identifying eriocheir sinensis. The molecular specific primer for identifying eriocheir sinensis has a forward primer PXF shown asSEQ ID NO: 1, and a reverse primer PXR shown as SEQ ID NO: 2. By utilizing the molecular specific labeled primer PXF / PXR, eriocheir sinensis can be subjected to rapid molecular identification by a conventional PCR method. The method is simple, is capable of finishing detection with PCR technology and is an effective assistance for identifying eriocheir sinensis through morphological characteristics.

The invention discloses an Eriocheir sinensis Microsporidia in-situ hybridization detection probe and kit. The sequence of the probe is disclosed as SEQ ID NO.1. After the probe is labeled by digoxin and combined with Microsporidia 18S subunit ribosome DNA (18S SSU rDNA) by hybridization, the Microsporidia infection can be judged under a microscope after alkaline phosphatase detection system color development. The invention also discloses a kit containing the probe, which has favorable specific, is simple to operate and can intuitively combine the pathogen detection and pathological changes. On the premise of efficiently and accurately detecting the Spiroplasma, the kit can analyze the infection rate and infection intensity of the sample section. The hybridization between the probe and 18S SSU rDNA is specific, the signal is progressively amplified, and thus, the kit disclosed by the invention is more sensitive and accurate than the conventional dyeing observation detection. The detection result proves that the section subjected to hybridization color development can be stored for a long time.

The invention discloses a primer pair for detecting Metschnikowia bicuspidate and application of the primer pair. Nucleotide sequences of the specific primer pair for detecting the Metschnikowia bicuspidate are shown as SEQ ID NO.1 and SEQ ID NO.2. According to the primer pair for detecting the Metschnikowia bicuspidate and application of the primer pair, specificity experiment results show that the primer pair only has specific fragments in the Metschnikowia bicuspidate, has no cross reaction with other common saccharomycetes and bacteria, and has good specificity; sensitivity experiments show that the minimum limit of the primer pair for detecting the Metschnikowia bicuspidate is 8.62 * 10 / micro L, and the primer pair has good sensitivity; and the primer pair provides an effective technical means for the detection or diagnosis of the Metschnikowia bicuspidate disease, by a method, the detection method of the Metschnikowia bicuspidate is simpler and more convenient, the diagnosis ismore accurate, and the method has an important significance in prevention and control of the Metschnikowia bicuspidate disease of economically cultured species such as Eriocheir sinensis, Macrobrachium rosenbergii, Chenook salmon and the like.

The invention discloses a pharmaceutical composition for treating mitten crab bacteriosis, which comprises the following traditional Chinese medicines in parts by weight: 10-30 parts of epimedium, 15-30 parts of humifuse euphorbia herb, 5-15 parts of Eucalyptus robusta, 10-25 parts of herba houttuyniae, 2-10 parts of Hinoki leaf and 5-20 parts of onion. The invention also discloses a preparation method of the pharmaceutical composition. The epimedium, humifuse euphorbia herb, herba houttuyniae and the like in the pharmaceutical composition have the antimicrobial and bacteriostatic effects; and when the epimedium, humifuse euphorbia herb, herba houttuyniae and the like are used as the effective components, the pharmaceutical composition can well prevent and treat mitten crab bacteriosis, and the effective rate is up to 99%.In the preparation method, the epimedium is extracted by a supercritical CO2 extraction process, so that the contents of the effective components (icariine and naphtha) in the epimedium are higher. The preparation method is simple and easy to implement and suitable for large-scale production.

The invention discloses an eriocheir sinensis reovirus RT-LAMP detection reagent kit and a detection method thereof. According to the detection method, primers needed for an EsRV RT-LAMP reaction system are designed according to a conservative region of the sequence of an eriocheir sinensis reovirus(EsRV) disclosed in a GenBank, and the detection primers include a pair of outer primers and a pair of inner primers. The detection reagent kit comprises the detection primers, RT-LAMP amplified reaction liquid, DNA polymerase, AMV reverse transcriptase and a reference substance. The reagent kit further comprises a color developing agent. Compared with a common detection technology of the virus, the reagent kit is easy, convenient and fast to operate, high in specificity, high in detection sensitivity, suitable for on-site detection of front-line production technicians and farmers and favorable for diagnosis and prevention of eriocheir sinensis tremor diseases and has wide application prospects.

The invention relates to the technical field of molecular biology and biochemistry, and discloses in-vitro gene recombination and expression technology of an eriocheir sinensis cysteine protease inhibitor (Escystatin) and function identification thereof. The eriocheir sinensis cysteine protease inhibitor is provided with a nucleotide sequence SEQ ID No.1 and a nucleotide sequence SEQ ID No.2. The invention also discloses inhibition effect of the eriocheir sinensis cysteine protease inhibitor on papain. The recombinant protein obtained by using the technology has potential application value in the development of antibacterial medicaments, feed additives and the like.

The invention discloses a practical rotifer strengthening method for medium trace elements-copper and manganese in eriocheir sinensis larva. The method specifically comprises the steps of preparing highly concentrated chlorella (the wet weight is (2-8)*1010 cells / g), adding a copper sulphate solution with the concentration of 1000-2000mg / L and a manganese sulfate solution with the concentration of 500-1000mg / L, enriching a marine chlorella solution with the total Cu amount of 400-800ug / g and a marine chlorella solution with the total Mg amount of 150-300ug / g for 3-6 hours, and using the enriched marine chlorella for strengthening the rotifers for 10-12h, wherein the quantity relative ratio of the chlorella to the rotifers is 5*103 cells / ind. The rotifers strengthened by the method are used for feeding the eriocheir sinensis larva, so that the trace elements, copper and manganese, are effectively supplemented, metamorphosis development can be stimulated, the eriocheir sinensis larva are developed into megalopa in a metamorphosis manner before a natural state, and the artificial breeding yield and quality of eriocheir sinensis megalopa are increased.

The invention belongs to the technical field of nondestructive testing, and relates to a nondestructive testing method for the internal eating quality of Eriocheir sinensis based on a smart phone. The method comprises the following steps: taking Eriocheir sinensis in different mature periods as samples, acquiring spectral data of crab meat, hepatopancreas, female crab gonad and male crab gonad ROI of the samples by utilizing a spectral data acquisition system based on the smart phone, and measuring the contents of characteristic chemical substances of the samples; carrying out SG smooth filtering preprocessing on the spectral data; according to the maturity of the samples, combining with the characteristic chemical substance content of crab meat, hepatopancreas, female crab gonad and male crab sex to formulate the grading index of the internal eating quality of the Eriocheir sinensis; enabling the preprocessed spectral data to be in one-to-one correspondence with the characteristic chemical substance content data, and obtaining the characteristic wavelength corresponding to each part of the sample through an algorithm; constructing a prediction model according to the characteristic wavelength data and the edible quality grading indexes. The method has the advantages of accuracy, high efficiency, no damage and real-time performance, and makes up the vacancy of the grading standard of the internal edible quality of the commercial crabs.

The invention discloses an Eriocheir Sinensis male specific Etse gene and application thereof. The nucleotide sequence of the Eriocheir Sinensis male specific Etse gene is shown as SEQ ID NO: 1, the amino acid sequence of encoded protein is shown as SEQ ID NO: 2, male specific molecular marker primers are shown as SEQ ID NO: 5 and SEQ ID NO: 6, and the Eriocheir Sinensis male specific Etse gene, the encoded protein or the male specific molecular marker primers are used for sex identification of Eriocheir Sinensis. An identification method comprises the following steps: carrying out PCR amplification by adopting the male specific molecular marker primers, carrying out electrophoresis detection after the reaction is finished, and displaying that the Eriocheir Sinensis with a specific band ismale and the Eriocheir Sinensis without the specific band is female by virtue of an electrophoresis result. The method is simple and efficient, and the sex determination of the Eriocheir Sinensis canbe quickly realized at the molecular level.

The invention discloses a specific probe for detecting hemocyanin gene expression of Eriocheir sinensis and application of the specific probe. Based on the principle that a probe sequence is specifically combined with mRNA of a target gene in proportion, a fluorescence in-situ hybridization technology is combined with image flow cytometry, different groups are distinguished according to whether fluorescence signals exist in cells or not, functional differences are analyzed according to signal strength, and the target gene can be identified according to the functional differences. And whether different genes are expressed in the same cell or not can be known by using multicolor fluorescence co-localization. Cell classification serves for diagnosis, function research and other requirements. Conventional methods mostly adopt antibody recognition to classify cells, but the current situation that aquatic animals lack commercially available antibodies limits the application. The method does not need an antibody, starts from a gene sequence, prepares a fluorescently-labeled short probe, is mild in hybridization condition, can carry out quantitative analysis at a complete single cell level, and has a good application prospect in cell classification and other aspects.

The invention discloses a superlarge-scale economic larva-breeding method for parents of eriocheir sinensis in the technical field of artificial breeding of eriocheir sinensis larvae. The method comprises the following steps: (1) selecting the parents of the eriocheir sinensis, and carrying out temporary rearing, wherein the weight of the female eriocheir sinensis is more than 250g, and the weightof the male eriocheir sinensis is more than 350g; (2) carrying out mating and overwintering on the parents of the eriocheir sinensis; (3) hanging egg-carrying eriocheir sinensis in a cage; and (4) breeding the larvae. According to the method, a novel breeding technique is used in each propagation stage of the eriocheir sinensis, and a traditional breeding method is updated, so that a series of problems of low egg-carrying rate of the parents and overwintering survival rate, difficulty in metamorphosis of the larvae and low survival rate in a superlarge-scale breeding process of the parents ofthe eriocheir sinensis in the past are solved, the deadlock of a technical blank in the superlarge-scale propagation technique of the eriocheir sinensis is broken through, and a set of mature technical system for the superlarge-scale breeding of the parents is established.

The invention discloses a mixed culture method for young eriocheir sinensis and colored carps. The method comprises the steps of (1) setting of pond conditions of mixed culture, (2) eriocheir sinensis seed breeding, (3) feeding management at the young eriocheir sinensis phase, (4) colored carp breeding, and (5) feeding management in the mixed culture period. According to the mixed culture method for the young eriocheir sinensis and colored carps, the yield and specifications of young eriocheir sinensis culture can be ensured, the survival rate and the culture specifications of the colored carps are ensured, and comprehensive pond production benefits can be better improved.

The invention relates to an Eriocheir sinensis quality estimation method based on unmanned aerial vehicle image processing, and the method comprises the steps: firstly photographing Eriocheir sinensisimages on site through an unmanned aerial vehicle, and obtaining a plurality of high-quality Eriocheir sinensis images; secondly, preprocessing the image, including image correction, image noise reduction and target extraction; then, for the Eriocheir sinensis target area, using Wiener filtering to restore the target area, performing edge detection by using a Canny operator after the original image is updated, and extracting an Eriocheir sinensis feature area; and finally, calculating the pixel area of the feature region, establishing a quality estimation model for the quality and crustaceanarea of the Eriocheir sinensis by using multiple regression models, performing error analysis, and preferentially selecting the estimation model. Compared with a traditional eriocheir sinensis qualityestimation method, the eriocheir sinensis quality estimation method has the advantages that the stress reaction of eriocheir sinensis caused by the traditional method is effectively avoided, the estimation precision is high, and the high requirement of the modern aquaculture industry for Eriocheir sinensis cultivation refinement management can be met.

The invention discloses an authenticity identification method and system for Eriocheir sinensis based on deep learning, and belongs to the technical field of deep learning. The method comprises the following steps: acquiring image data and identity (ID) of the eriocheir sinensis; preprocessing the image data to obtain a target image, and obtaining an original image according to the identity ID; respectively calculating the target image and the original image to obtain feature vectors, and calculating the similarity between the target image and the original image according to the feature vectors of the target image and the original image; and judging the authenticity of the Eriocheir sinensis corresponding to the target image according to the similarity. The system comprises a memory and a processor, wherein the processor executes a program stored in the memory to realize the method. According to the method, the defect that the authenticity identification accuracy of the eriocheir sinensis in the prior art is low is overcome, the authenticity identification of the eriocheir sinensis under any environmental condition can be realized, and the authenticity identification accuracy of the eriocheir sinensis can be further greatly improved.

The invention discloses lactobacillus plantarum CB5. The CB5 is preserved in the China Center for Type Culture Collection, the deposit date is April 8, 2019, and the deposit number is CCTCC NO: M 2019244. The invention also discloses a microbial agent. The invention also discloses the lactobacillus plantarum CB5 and application of a microbial agent thereof, and the lactobacillus plantarum CB5 andthe microbial agent thereof have significant antibacterial activity against aquatic animal pathogenic bacteria, are used for improving the immune function of eriocheir sinensis, significantly reduce the content of ammonia nitrogen and nitrite in the water of cultured aquatic animals, and have the function of purifying water bodies.

The invention discloses an eriocheir sinensis feed additive and a method for improving the fatness of eriocheir sinensis by using the same. The additive comprises a bdellovibrio bacteriovorus microcapsule with the concentration of 3.69*10<7> pfu / g to 3.70*10<7> pfu / g, a bacillus amyloliquefaciens microcapsule with the concentration of 4.37*10<6> cfu / g to 1.91*10<9> cfu / g and a feed bonder, whereinthe mass ratio of the bdellovibrio bacteriovorus microcapsule to the bacillus amyloliquefaciens microcapsule to the feed bonder is 16:(1-4):(8.5-10). The invention furthermore provides the method forimproving the fatness of the eriocheir sinensis by using the additive. The additive disclosed by the invention is faster in effect taking and more remarkable in effect, and the application and popularization of the additive is more beneficial for improving the quality and market competitiveness of the eriocheir sinensis, reducing the breeding cost of farmers, increasing the breeding benefit and relieving the secondary pollution of chemical disinfectants to aquaculture water.

Provided is an acoustic tag tracking method for parent crabs of Yangtze estuary eriocheir sinensis. The method is characterized by comprising the steps that acoustic tags are fixed to back shells of the parent crabs; evaluation tests are carried out on the signed crabs, and if the signed crabs are not obviously different from a control crab, it indicates that the signed crabs can be used for release study; areas coinciding with the migration site of the natural population parent crabs in the same day are selected as the release water area of the signed crabs, longitude and latitude data of a release site are recorded through a GPS instrument, and release time is recorded; after a headphone finds acoustic signals in the signal monitoring stage, a directional monitoring headphone is put at the position two meters deep in water, and a monitor records the signal strength and time of all the acoustic signs; the received signal strength is converted into the position of the signed crabs according to the regression equation of the signal strength of the acoustic signs and the distance, and the GPS data of the signed crabs are recorded; the migration speed of each signed crab is calculated according to a migration speed formula and the GPS data, and the moving positions of the parent crabs are connected to obtain a migration route; when the signed crabs reach a spawning ground, the signed crabs stay still and carry legs for reproduction, and the water area is the spawning ground of the parent crabs.

The invention discloses a method for judging high-quality adult eriocheir sinensis, and which belongs to the field of aquaculture science. The method comprises the following steps: measuring the width of the adult eriocheir sinensis by using a vernier caliper and recording as A, measuring the quality of the adult eriocheir sinensis by using an electronic scale and recording as B, and judging that the adult eriocheir sinensis is high-quality according to the condition that B / A of female crabs is greater than 2.6; and judging that the adult eriocheir sinensis is high-quality if the B / A of the male crabs is greater than 3.1, and judging the quality of adult eriocheir sinensis. The ratio of the weight of the dehydrated adult crabs to the width of the crab shells is adopted for accounting, and the method is simple, easy to implement, convenient and reliable. Under the condition of the same crab shell width, the weight is larger when muscles in adult crabs are fuller and hepatopancreas are richer so that the ratio of the weight to the crab shell width value is larger. On the premise that crab bodies are not damaged, the basic values of the adult crabs are utilized to judge the high-quality adult crabs of the eriocheir sinensis.

The invention discloses a cultivating technology of Eriocheir sinensis megalopas in an inland freshwater lake and belongs to the field of aquaculture. The invention provides the cultivating technology of the megalopas of Eriocheir sinensis by controlling key steps such as culture pond preparation, sterilization and water quality regulation and daily management; by implementing the cultivating technology, the cultivated megalopas are low in cultivation cost, young Eriocheir sinensises are high in survival, consistent in transformation time, and neat in height and can be cultivated on a large scale.

The invention relates to the technical field of aquaculture, in particular to a culture method for improving the quality of eriocheir sinensis. The culture method comprises the following steps of transforming a pond; planting aquatic plants; putting juvenile crabs; feeding baits; regulating and controlling water quality and oxygenating; fattening adult crabs and the like. According to the culturemethod, the water purification fishery breeding technology is utilized, the modes of water quality regulation and control, organic source bait cooked food feeding, supplementary feeding of detoxifiedpurple sweet potatoes, artificial seawater domestication and weight gaining in a temporary breeding pond and the like are mainly adopted, and therefore the common problems of small specification, poorquality and low economic benefit of crab culture in a hairy crab pond are solved; and the method can remarkably improve the economic benefit, the environmental benefit and the ecological benefit of hairy crab culture.

The invention discloses a method for breeding and producing a new variety of a commercial line of eriocheir sinensis. The method comprises the following steps: (1) parent selection of the commercial line: selecting commercial selection lines A and B of eriocheir sinensis for serving as parents; (2) production of the commercial line: performing diallel crossing on the commercial selection lines A and B to produce the commercial line; (3) screening of the commercial line: screening an A-female and B-male commercial line which takes line A as a female parent and takes line B as a male parent through growth contrast test; (4) performing productive verification on the commercial line; (5) applying the new variety of the commercial line. By adopting the new variety of the commercial line bred and produced through the method, the major advantages of the female parent A and the male parent B are well combined, high growth speed, high group yield and remarkable hybrid vigor are realized, and excellent provenance is provided for the river crab industry in China. The new variety is the first variety of the commercial line of eriocheir sinensis in China.

The invention discloses double-stranded RNA for improving the disease resistance of eriocheir sinensis and application thereof. The RNAi vector of the important gene EsMMP-14 participated in natural immunity, cell proliferation and tissue generation of the eriocheir sinensis is constructed, and the vector can be used for improving the disease resistance of the eriocheir sinensis, and has the characteristics of environmental friendliness and higher commercial application value.