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Rotifer strengthening method for stimulating eriocheir sinensis larva growth

A technology for the development of Chinese mitten crabs and larvae, which is applied in animal feed, animal husbandry, application, etc., can solve the problem that the strengthening method of water-soluble trace elements has not been established, and achieve the effect of promoting metamorphosis and improving yield and quality.

Inactive Publication Date: 2013-05-29
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, fortification of rotifers with fatty acids and vitamins cannot be directly applied to trace minerals, while fortification with water-soluble trace elements has not been established

Method used

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  • Rotifer strengthening method for stimulating eriocheir sinensis larva growth
  • Rotifer strengthening method for stimulating eriocheir sinensis larva growth
  • Rotifer strengthening method for stimulating eriocheir sinensis larva growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Rotifers directly absorb trace elements copper and manganese

[0016] The test uses a water tank with a volume of 30L, which is filled with 25 L of seawater with a salinity of 20‰, and the water tank is set to add copper at a concentration of 50 μg L ?1 , the concentration of manganese is 50 ug L -1 , set a control group without adding copper and manganese, concentrate the cultured rotifers into a high-density rotifer liquid with a 150-mesh sieve, then take 1 mL of dilution and count, adjust to a density of 800 per milliliter, and use a concentration of 4 per milliliter. ×10 6 Chlorella cells are fed to rotifers to maintain their normal physiological state. After 12 hours, the concentrations of copper and manganese in rotifers were determined to be 6.48±0.2 and 9.50±0.2 μg g ?1 (dry matter), there was no significant change compared with the control group.

Embodiment 2

[0017] Example 2 Seawater Chlorella Enrichment of Trace Elements Copper and Manganese

[0018] Seawater chlorella is harvested in the exponential growth period, concentrated by LXJ-H centrifuge, and stored in the refrigerator (no more than 24 hours). Add appropriate amount of algae mud to the rotifer culture solution to prepare highly concentrated chlorella (wet weight, 7×10 10 cells / g); use deionized water to prepare 1000mg / L, 1500mg, 2000 mg / L copper sulfate solution and 500mg / L, 750mg / L, 1000 mg / L manganese sulfate solution respectively. Take 8 plastic test tubes numbered 1-8, add 10 g of chlorella solution to each, and add 4 mL of copper sulfate solution with a concentration of 0 mg / L, 1000 mg / L, 1500 mg, and 2000 mg / L to test tubes 1-4 respectively, Then add 3mL manganese sulfate solution with a concentration of 0mg / L, 500mg / L, 750mg / L, and 1000 mg / L in turn, and place the test tubes at 23°C to enrich the chlorella in test tubes 1-4 with trace elements copper and Mang...

Embodiment 3

[0020] Embodiment 3 Rotifer strengthening

[0021] No. 3 chlorella test tube enriched with trace elements copper and manganese in Example 2 and 2 mg / L emulsified fish oil to strengthen the body length of 200-300 um Brachionus rugosa ( Bracgionus plicatilis ), the ratio of 25L chlorella to rotifers is 5×10 3 cells / ind (add 50 mg emulsified fish oil), so that the body contains suitable trace elements and unsaturated fatty acids. Sampling was carried out at 1, 3, 6, 12, and 24 hours respectively, and according to GB / T5009.168-2003 and GB / T5009.17-2003, fatty acid content and trace element content in rotifers after nutritional fortification were detected, and the results were as follows: figure 1 , 2 shown. It can be seen from the figure that the main n-3 higher unsaturated fatty acid (high unsaturated fatty acid, HUPA) and trace elements copper and manganese content in rotifers increased significantly. The nutrient requirements of trace elements copper and manganese and fat...

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Abstract

The invention discloses a practical rotifer strengthening method for medium trace elements-copper and manganese in eriocheir sinensis larva. The method specifically comprises the steps of preparing highly concentrated chlorella (the wet weight is (2-8)*1010 cells / g), adding a copper sulphate solution with the concentration of 1000-2000mg / L and a manganese sulfate solution with the concentration of 500-1000mg / L, enriching a marine chlorella solution with the total Cu amount of 400-800ug / g and a marine chlorella solution with the total Mg amount of 150-300ug / g for 3-6 hours, and using the enriched marine chlorella for strengthening the rotifers for 10-12h, wherein the quantity relative ratio of the chlorella to the rotifers is 5*103 cells / ind. The rotifers strengthened by the method are used for feeding the eriocheir sinensis larva, so that the trace elements, copper and manganese, are effectively supplemented, metamorphosis development can be stimulated, the eriocheir sinensis larva are developed into megalopa in a metamorphosis manner before a natural state, and the artificial breeding yield and quality of eriocheir sinensis megalopa are increased.

Description

technical field [0001] The invention relates to a rotifer nutritional strengthening method, in particular to a method for strengthening rotifers with trace elements copper and manganese for promoting the development of mitten crab larvae. Background technique [0002] Artificial breeding of mitten crab larvae requires a large amount of live bait, and artificially bred rotifers are generally used as live bait for shrimp and crab larvae. In the process of artificial breeding, the nutritional balance of the open bait in the development stage plays a key role in the seed production in the later stage. As prosthetic groups and metal binding sites of some enzymes, trace elements can maintain bone development, promote the body's immune function and maintain the normal progress of substance metabolism. The larval development of Eriocheir sinensis requires 40 mg / kg and 17 mg / kg of copper and manganese in the diet respectively, while the artificially bred rotifers have only 6 mg / kg,...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16
Inventor 孙盛明陈立侨戈贤平
Owner EAST CHINA NORMAL UNIV
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