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57 results about "Spliceosome complex" patented technology

A spliceosome is a large and complex molecular machine found primarily within the splicing speckles of the cell nucleus of eukaryotic cells. The spliceosome is assembled from snRNAs and SR protein. The spliceosome removes introns from a transcribed pre-mRNA, a type of primary transcript.

Method for detecting variable spliceosome in third generation full-length transcriptome

ActiveCN105389481AEfficient access to shear structuresPerfect commentSequence analysisSpecial data processing applicationsReference genome sequenceGene model
The invention discloses a method for detecting a variable spliceosome in a third generation full-length transcriptome. The method comprises the following steps: merging original annular test sequences with joints removed to form a monomolecular transcript sequence, and screening a third generation full-length transcript sequence; comparing the third generation full-length transcript sequence with a reference genome sequence, and screening a third generation full-length transcript sequence having coverage and similarity with the reference genome sequence larger than preset thresholds; carrying out splicing false positive filtration and DNA contamination filtration on the screened third generation full-length transcript sequence; and carrying out gene annotation and variable spliceosome annotation on the filtered third generation full-length transcript sequence. An overlong read length of a third generation sequencing technology mentioned in the method disclosed by the invention is large enough to cover most RNA, the third generation full-length transcript sequence can be obtained by SMRT sequencing transcriptomes without being assembled, and a splicing structure of a gene can be effectively obtained by third generation transcriptome sequencing, and more perfect gene model annotation can be constructed.
Owner:嘉兴菲沙基因信息有限公司

Methods and compositions for use in spliceosome mediated RNA trans-splicing

The present invention provides methods and compositions for delivery of synthetic pre-trans-splicing molecules (synthetic PTMs) into a target cell. The compositions of the invention include synthetic pre-trans-splicing molecules (PTMs) with enhanced stability against chemical and enzymatic degradation. The synthetic PTMs are designed to interact with a natural target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction resulting in the generation of a novel chimeric RNA molecule (chimeric RNA).
Owner:VIRXSYS

Reagent for diagnosing chronic myeloid leukemia blast crisis based on hnRNPA1 splicing variant and kit

The invention discloses application of No.8 exon skip / retained spliceosome (hnRNPA1-a and hnRNPA1-b) of hnRNPA1 in preparing a reagent for diagnosing chronic myeloid leukemia blast crisis or in a kitcontaining the diagnostic reagent. The reagent has higher sensitivity and specificity, can observe the chronic myeloid leukemia blast crisis more simply, accurately and early so as to timely take treatment means, and has higher clinical application value.
Owner:THE SECOND AFFILIATED HOSPITAL TO NANCHANG UNIV
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