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Primer, kit and detection method for detecting C1QTNF3 gene 219bp-deletion variable spliceosome

A technology of splice body and kit, which is applied in the field of detection of alternative splice body of C1QTNF3 gene 219bp deletion, which can solve the problems of low accuracy rate, cumbersome procedures, missing, etc., and achieve the effect of strong practicability

Pending Publication Date: 2019-05-31
SHANXI AGRI UNIV
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Problems solved by technology

[0006] In the process of cloning and functional research on the porcine C1QTNF3 gene, the inventor found that the first exon of the gene lacked a 219bp fragment, and this deletion conformed to the GT-AG splicing pattern. The primer for the spliceosome and the kit and detection method containing the primer can effectively solve the problems of cumbersome procedures, long time-consuming and low accuracy of the existing detection methods

Method used

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  • Primer, kit and detection method for detecting C1QTNF3 gene 219bp-deletion variable spliceosome
  • Primer, kit and detection method for detecting C1QTNF3 gene 219bp-deletion variable spliceosome
  • Primer, kit and detection method for detecting C1QTNF3 gene 219bp-deletion variable spliceosome

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Embodiment 1

[0030] Example 1 Method for Detecting C1QTNF3 Gene 219bp Deletion Alternative Splicing Body

[0031] The schematic diagram of the technical process of this embodiment is as follows figure 1 As shown, it mainly includes the following steps:

[0032] 1. Collection of tissue samples

[0033] Three 1-month-old Mashen pigs came from a pig breeding farm in Datong City, Shanxi Province. After slaughter, biceps femoris (GJ), psoas major (YJ), abdominal subcutaneous fat (PZ), heart (X), liver (G), spleen (P), lung (F), kidney (S ), stomach (W), etc., were immediately put into liquid nitrogen, and then stored in a -80°C refrigerator for later use.

[0034] 2. Tissue RNA extraction and cDNA synthesis

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Abstract

The invention provides a primer, kit and detection method for detecting C1QTNF3 gene 219bp-deletion variable spliceosome. The sequence of the primer for detecting the C1QTNF3 gene 219bp-deletion variable spliceosome is shown in SEQ ID NO:1 and 2. The for detecting C1QTNF3 gene 219bp-deletion variable spliceosome is separated from various splicing forms of the gene , the expression level of the C1QTNF3 gene 219bp-deletion variable spliceosome is quantitatively analyzed, the detection method is accurate, quick, convenient to use and high in practicability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer, a kit and a detection method for detecting a 219bp deletion alternative splicing body of C1QTNF3 gene. Background technique [0002] C1QTNF3 (CORS26), also known as CTRP3 or cartducin, is an adipokine discovered in 2003. The physiological function of C1QTNF3 is to regulate chondrocyte proliferation and bone and joint diseases; regulate endocrine, glucose and lipid metabolism, mitochondrial biosynthesis, immunity, inflammatory response, apoptosis, angiogenesis, vascular calcification and ventricular remodeling and other physiological and pathological process; regulate the secretion of testosterone and adipokines in the body. Porcine C1QTNF3 gene is widely expressed in tissues, but the identification and isolation of its various alternative splice forms is relatively slow, which hinders the study of the physiological function of C1QTNF3 gene to some extent. Therefore, it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858C12N15/11
Inventor 曹果清张雪莲张万锋乐宝玉张宁芳成志敏秦本源高鹏飞郭晓红蔡春波李步高
Owner SHANXI AGRI UNIV
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