The invention discloses an efficient method for actinomycete genome splicing. The method includes the steps that A, actinomycete is subjected to database building sequencing through a third-generation sequencing platform; B, the actinomycete is subjected to database building sequencing through a second-generation sequencing platform; C, stopping data of the third-generation sequencing platform is spliced; D, stopping data of the second-generation sequencing platform is spliced; E, the splicing results of the two platforms are subjected to collinearity analysis to obtain the connecting relationships between all sequences, and the sequences are connected depending on the relationships; F, the connecting result is corrected through the stopping data of the second-generation sequencing platform. According to the efficient method for actinomycete genome splicing, the advantages of third-generation sequencing and the advantages of second-generation sequencing are combined, the complete actinomycete genome splicing results are obtained, and the genomics basis is provided for revealing the physiological and biochemical characteristics and the metabolic rule of actinomycete genome splicing.