Third-generation sequencing data error correction method, third-generation sequencing data error correction device and computer readable storage medium
A sequencing data and next-generation sequencing technology, applied in the field of bioinformatics, can solve problems such as data loss, inapplicability of low-depth data, and no quality value system
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[0082] Example 1: Mixed error correction of human chromosome 22 PacBio sequencing data
[0083] The original data in this example are 68×Hiseq PE250 sequencing data and 90×Pacbio sequencing data. In this embodiment, we extracted 15×Pacbio sequencing data as example data to test the performance of the present invention under low-depth data.
[0084] 1. Using 68×Hiseq PE250 sequencing data and 15×Pacbio sequencing data, use DBG2OLC software for mixed assembly to obtain a preliminary reference genome.
[0085] 2. The statistical results of the data show that the N50 of the assembled contig (contig) is 289kb, and the self-alignment shows that there are no long-segment high-repetition sequences, and the assembly results are suitable for use as a reference sequence for alignment without any processing.
[0086] 3. Use the bwa software to compare the Hiseq sequencing data and the Pacbio sequencing data to the reference sequence.
[0087] 4. Use the maximum a posteriori model to inf...
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