The invention discloses a synthetic method of amino-substituted cyclohexyl crown ether, which belongs to the technical field of synthetic technique of organic compound. The synthetic method uses nitro-substituted benzo crown ether as a substrate, uses high-activity Pichler ruthenium as a catalytic agent, uses high pressure hydrogen to achieve reduction of nitro and hydrogenation of phenyl group in substrate molecules simultaneously in one-step reaction, and accordingly obtains needed amino-substituted cyclohexyl crown ether products. The synthetic method enables the existing two-step reaction to be merged in a same system, and operation is greatly simplified. The temperature and the pressure of the needed reaction are relative mild, occurrence of side reaction is well avoided, and purity of obtained products is guaranteed. The ruthenium catalytic agent is high in catalytic activity, while cost is relatively lower than that of rhodium, platinum and the like, and the synthetic method has the cost advantage. Simultaneously, after reaction ends, the catalytic agent can be well separated and recycled.