Method for detecting fusion gene based on transcriptome sequencing data
A technology of transcriptome sequencing and gene fusion, applied in the field of transcriptome analysis, to achieve the effects of improving efficiency and accuracy, reliable detection results, and high sequencing depth
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[0028] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention.
[0029] We applied an embodiment of the method described in the present invention to a soybean transcriptome project. In this project, the soybean transcriptome was sequenced using the third-generation ISO-seq sequencing method and the second-generation RNA-seq sequencing method. The sequencing samples were mixed samples of different soybean tissues and developmental stages. Among them, two libraries were established for the third generation of transcription, and the sizes of the libraries were 0.6-2.5kb and >1.5kb respectively. The two libraries were sequenced with the PacBio RSII sequencing platform to generate 16 cells and 7 cells respectively. A library was constructed by next-generation sequencing with a library size of 200bp, and a total of 6G ...
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