37 results about "DNA database" patented technology

The invention relates to the technical field of high-flux sequencing and discloses a database-building method for amplicon sequencing. The database-building method comprises the following steps of 1, object region enrichment based on PCR amplification: designing primer sequences aiming at an object region of genome DNA of a sample to be detected, wherein ends 5' of a forward primer and a reverse primer in the primer sequences are provided with random base sequences and linking sequences, carrying out PCR amplification and recovering the PCR product, and 2, sequencing linker introduction based in PCR amplification: carrying out mixing on the PCR product obtained by the step 1, designing primer sequences comprising sequences complementary with the linking sequences obtained by the step 1, carrying out PCR amplification on the mixture, and recovering the PCR product so that a DNA database for amplicon sequencing is obtained. The database-building method reduces building time and cost of the DNA database for amplicon sequencing, reduces error of bioinformatics analysis of the sequencing result and improves accuracy and authenticity of the analysis result.

The invention discloses a single-chain molecular identifier adapter and a single-chain DNA database creating method and application thereof to circulating tumor DNA detection. The single-chain molecular identifier adapter comprises a stem structure formed by 14 base pairing, a sequencing primer sequence and a molecular identifier sequence formed by 8 random nucleotides, wherein a base 'U' is inserted between the sequencing primer sequence and a stem structure sequence, and a stem-loop structure can be formed by annealing denaturation of the single-chain nucleotide sequence. By single-chain DNAdatabase creating, technical problems of low sensitivity, fragmentation, low concentration and short ctDNA fragments in liquid biopsy in application of an existing detection technique are solved. Accurately distinguishing whether sequencing reads identical in genome coordinate beginning and end loci come from cfDNA released by the same one or multiple germinal cell is realized, sequencing reads derived from original positive and negative chains of the same cell are paired for analysis, and accordingly sequencing rehandling is reduced, and the distinguishing rate of false positive mutation isincreased.

The invention discloses a human STRtyper PCR enlarged fluoroscopic examination reagent kit with quick speed detecting, accuracy, good repeatability, good versatility, low price and practicality. The applicability of the reagent kit for Chinese is remarkable in particular. The serial products of the invention comprise the three following kits: (1) a STRtyper-20G kit with the largest STR gene locus of the global and most powerful recognizing ability; (2) a STRtyper-16GC kit which is more suitably used to build a criminal DNA database of Chinese population and identify the identity; (3) a STRtyper-10F / G kit with brand new STR gene locus. Each kit product comprises the component of 1.1 ml * 1 tube of STR PCR reaction mixture; 0.55 ml * 1 tube of STRtyper primer mixture; 120 microlitre * 1 tube of HS-Taq DNA polymerase; 0.3ml * 1 tube of STR control DNA; 50 microlitre * 1 tube of STRtyper allelomerphic gene mixture; 0.5 ml * 1 tube of internal standard; 30 microlitre* 1 tube of 310 spectrum correcting standard; 30 microlitre * 1 tube of 3100 spectrum correcting standard.

The present invention discloses a method and a system for monitoring and tracing transmission of multimedia resources, wherein, said method comprises: capturing data packets in a network link, reassembling the captured data packets to complete the restoration of data streams, and storing the restored data streams into a data stream database; and extracting data fragments from a multimedia file as file DNA fragments to construct a file DNA, and storing the file DNA into a file DNA database; and invoking the file DNA from the file DNA database, and identifying the file DNA fragments of the file DNA in the data streams to get identification results.

The invention discloses a human Y chromosome 37 STR loci fluorescence labelled multiplex amplification kit and an application thereof. The kit comprises a specific primer for amplifying 37 Y-STR lociwhich comprise 30 low-mutation Y-STR loci and 7 rapid-mutation Y-STR loci. The kit comprises 30 low-mutation and 7 rapid-mutation rate high-polymorphism Y-STR loci, considers distinguishing of checking of a male family and different male individuals of a same male line, and is the Y-STR detection product with a maximum loci quantity on market. The primer in the kit has the advantages of strong specification, high sensitivity, and accurate parting result, and practical case examination, DNA database construction and paternity identification requirements can be completely satisfied. The kit hasstrong check-material adaptability.

The invention discloses a method and a system for monitoring and tracking multimedia resource transmission, comprising a data acquiring module for capturing a data packet in a network linkage; a data stream recovery module for regrouping the captured data packet to complete the recovery of the data stream and storing the recovered data stream in a data stream database; a file DNA extraction module for extracting file DNA segments from multimedia file to form a file DNA to store in a file DNA database; and a file DNA recognition module for invoking the file DNA from the file DNA database and recognizing the file DNA segments of the file DNA from the data stream to obtain a recognition result. The method and system of the invention can be used for monitoring the dynamic transmission process of the multimedia resource.

The invention discloses a gene sequencing data compression and transmission method. The method comprises the following steps of A, establishing a standard DNA sequence database; B, deploying the standard DNA sequence database to a data processing device; C, preprocessing DNA sequencing data: comparing the DNA sequencing data with the standard DNA database one by one, generating a corresponding relationship, replacing an original text of the DNA sequencing data with numbers of the standard DNA database, and separately storing a part, different from the standard DNA database, of the DNA sequencing data; D, performing compression; and E, performing storage or transmission. The standard DNA sequence database is stored in the data processing device, so that a large amount of information contained in the DNA sequencing data can be represented by the numbers of the standard DNA database, and the data capacity after the step of preprocessing the DNA sequencing data is greatly reduced; through further compression, the capacity is smaller, so that the storage space of the DNA sequencing data is smaller, and the data transmission efficiency is higher; and the data in the method is matched with output data of a second-generation sequencing technology and even a third-generation sequencing technology.

The invention relates to a kit for predicting colorectal cancer liver metastases and a use method. The kit includes a DNA database building kit, the DNA database building kit comprises probes of a plurality of genes, and the plurality of genes include: high risk genes: KRAS, BRAF, MLH1, NRAS, MSH2, PMS2, UGT1A1, MSH6 AKT1, PIK3CA, PTEN, SMAD4, TP53, NM23, TIAM1, MTS1; and low risk genes: PRKDC, RAD50, STAG2, XRCC5, XRCC6, FANCA, ATR, MUTYH, EMSY, ERCC4, RAD51, PARP1, XRCC1. The kit provided by the invention performs related mutation detection on colorectal cancer liver metastases related genes in peripheral blood, and combines specific scoring mechanism to rapidly and conveniently judge and predict colorectal cancer liver metastases.

The present invention relates to a fluorescence labeled composite amplification detection kit for simultaneously analyzing 17 gene loci of canine genomic DNA. With the system provided by the kit, genetic polymorphisms of the 17 gene loci of the canine genomic DNA can be simultaneously analyzed. The 17 gene loci of the canine genomic DNA comprise DAmel, PEZ1, PEZ2, PEZ3, PEZ5, PEZ6, PEZ8, PEZ12, PEZ15, PEZ20, PEZ21, FH2010, FH2054, FH2079, FH2132, FH2611 and VWFX, is divided into four groups, and relates to the fluorescence labels comprising five colors. The fluorescence labeled composite amplification detection kit provided by the present invention is provided for composite amplification of a mixture comprising oligonucleotide primers of the 17 gene loci. In the system, the gene locus foridentifying the gender of the DNA samples is the DAmel gene locus. The detection results of the system has characteristics of high polymorphism, good balance, high sensitivity, strong specificity, accurate typing result, strong species-specificity, and can fully meet the requirements of dog individual identification, paternity identification, and DNA database construction.

A method for identifying unculturable microorganisms in which at least one bacterial cell from an environmental sample containing a plurality of microorganisms is isolated, at least one DNA fragment from the at least one bacterial cell is amplified, cloned into at least one E. coli vector and sequenced, resulting in identification of at least one DNA sequence. The at least one DNA sequence is compared with existing DNA databases, resulting in identification of the at least one DNA sequence as derived from either an unculturable microorganism or a known microorganism.

The invention discloses a method for identifying a salmon variety in a salmon can, in particular a method for identifying the salmon variety in the salmon can with the application of a micro-barcode technology; the method mainly comprises the following steps: a) weighing a certain mass of a salmon can sample and extracting DNA; b) conducting amplification by virtue of a micro-barcode primer by taking the DNA obtained in the step a) as a template, so as to obtain a DNA segment of 212bp; and c) sequencing the segment which is obtained through amplification in the step b), and conducting sequence characteristic contrast with an existing DNA database, so that information on the salmon variety is obtained. The method for identifying the salmon variety in the salmon can with the application of the micro-barcode technology established by the invention has very important practical significance to the improvement of product quality and safety levels of China's canning industry, the standardization of industry development and the protection of consumers' lawful rights and consumers' rights to know.

The invention provides a DNA database establishing method for high-throughput sequencing, and particularly discloses a cfDNA database establishing method. On the basis of a standard database establishing method of Ion AmpliSeq Library Kit 2.0, the agent use amount and the PCR amplification conditions are optimized, and therefore reaction consumption is reduced, amplification specificity is higher, and the initial DNA amount is lower and even lower than 1 ng.

The invention discloses function candidate gene for affecting the meat quality trait of a pig and a screening method of t function candidate gene. The function candidate geneisRXRG, GPI, CPT2 and / or GABARAPL1. The screening method comprises the following steps: (1), selecting longissimus muscles of two extreme pig breeds with large meat quality trait difference, extracting DNA, and independently creating databases; (2), performing whole genome DNA methylation sequencing on sample DNA databases by adoptingan MeDIP-Seq technology; (3), obtaining maps of whole genome DNA methylation levels of the two extreme pig breeds, and screeningdifferentialmethylationexpressed genes; (4), performing GO functional annotation and KEGG Pathway analysis on the differentialmethylationgenes, and screening out functional genes and regulatory pathways related to meat quality traits, such as fatty acid formation and decomposition; (5), performing DNA methylation and expression profileconjoint analysis through MeDIP-seq and RNA-seqtest results, and obtaining the function candidate gene of the meat quality trait of the pig. According to the method, the function candidate geneparticipatinginregulationof the meat quality trait of the pig is screened out, and a foundation is laid for the future deep research.

The invention discloses a fluorescently-labeled multiplex amplification kit for 35 STR loci of a human Y chromosome and application of the fluorescently-labeled multiplex amplification kit. The fluorescently-labeled multiplex amplification kit comprises specific primers for amplifying the 35 Y-STR loci, wherein the 35 Y-STR loci comprise 28 low-mutation Y-STR loci and 7 rapid-mutation Y-STR loci.The kit provided by the invention comprises the 28 Y-STR loci with the low mutation rate and the 7 rapid-mutation Y-STR loci with high polymorphism, and male family investigation and distinguishing between different male individuals in the same paternal line are balanced; the primers in the kit have the advantages of strong specificity, high sensitivity and accurate typing result, and can completely meet the requirements of actual case inspection, DNA database construction and paternity test; and the kit has very strong test material adaptability.

The invention discloses an intelligent biological recognition automatic blood sampling system combined with the network technology. The system comprises an intelligent biological recognition apparatusand an automatic blood sampling apparatus, wherein the intelligent biological recognition apparatus comprises a camera, a biological radio frequency sensor, a Type B non-contact IC card reader, an information digital memory and a bar code printer, the camera, the biological radio frequency sensor and the Type B non-contact IC card reader collect identity information and store the identity information into the digital memory, and the bar code printer is used for printing a blood sampling card with the identity information; and the automatic blood sampling apparatus comprises a full-automatic blood sampling arm, a blood sampling needle telescopic cylinder, a blood sampling needle clamping apparatus, a blood sampling needle storage bin automatic feeding apparatus, a blood sampling card clamping apparatus, a blood sampling card storage apparatus, a blood sampling auxiliary apparatus, a motor and a control apparatus. According to the system, the accuracy of the identity information of a detected person is guaranteed, the workload and occupational exposure risks of medical staff are reduced, a non-contact blood sampling self-service mode between blood sampling and supplying persons is provided, and a large-scale intelligent medical treatment and DNA database can be constructed.

The invention provides a primer group and a kit for simultaneously amplifying 37 Y-STR gene loci of human and application thereof, and belongs to the technical field of molecular genetics. In the invention, the 37 Y-STR gene loci comprise 36 Y chromosome STR gene loci and 1 Y-indel gene locus; 37 gene loci can be amplified in one reaction at the same time, the compatibility of current public security DNA database comparison is fully met, the amplification time is shortened, the identification efficiency and the detection material adaptability of the kit are improved, and the multifunctional STR identity identification requirements of current forensic identity identification, forensic DNA database construction and judicial genetic relationship identification can be met.

The invention discloses a multiple amplification detection kit for 21 short tandem repeat sequences using a novel bifluorescence marking method. The kit comprises a compound primer group comprising 21 pairs of specific primers, 21 gene bases for compound amplification, a reaction mixed liquid and a warm start Taq enzyme, wherein the first basic group at 5' end of a labeled primer is marked by a conventional dye, and some basic groups at 5' and 3' ends of the primer are marked by a short excitation wavelength fluorescent dye, so that fluorescent energy transfer is achieved. The amplification result disclosed by the invention is more stable and balanced, and meanwhile, the kit is higher in sensitivity, can be widely applied to individual recognition, DNA database building and paternity test of Chinese population, and is suitable for various detection materials.

The invention discloses a method for coloning and determining beta- galactosidase gene. The invention deletes nucleotide squence carried on vector, at 334- 489 position with its length being 156bp by using adverse PCR technique based on vector pUC18 and pUC19, and produces vector pUC18 (lac-) and pUC19 (lac-). The vector pUC18 (lac-) and pUC19 (lac-) are used to construct DNA database, and determines and clone recon that contains beta- galactosidase gene according to the blue or white bacteria group formed on LB culture medium plate of IPTG and X-gal, and determines promotor type for beta- galactosidase gene according to if IPTG induction is necessary for blue bacteria group formation.

The invention discloses a fluorescence mark composite amplification detection kit for simultaneously analyzing 16 loca of cattle. The fluorescence labeling composite amplification detection kit is characterized by comprising the 16 loca including TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA023, CSSM66, ETH3, ETH225, BM1824, BM1818, BAmel, G18833 and BM720, the loca are divided into four groups, and fluorescence marks of five colors are involved totally. The fluorescence mark composite amplification detection kit provides an oligonucleotide primer mixture used for conducting composite amplification on the 16 loca; in a system, the included locus used for identifying the sex of a DNA sample is a BAmel locus. The detection result of the system shows that the fluorescence mark composite amplification detection kit is high in polymorphism, good in balance, high in sensitivity and specificity, accurate in typing result, high in species specificity and capable of completelymeeting the demands for cattle individual identification, paternity identification and DNA database construction.

The invention discloses a silicon-based SERS chip DNA database constructing and training method used for artificial intelligence detection of DNA. The silicon-based SERS chip DNA database constructingand training method used for artificial intelligence detection of DNA comprises the following steps: preparing a silver nanoparticle modified silicon-based SERS substrate through a hydrofluoric acidassisted etching method; constructing a SERS database of DNA; and extracting main feature values used for a deep neural network for the SERS database, and training the deep neural network. The detection method disclosed by the invention can be carried out at room temperature; the operation is safe; the recognition rate of the DNA target can reach 86.11%; and the invention has good specificity, reproducibility and convenient detection process.

The invention aims at solving the technical problem in the prior art that analysis data of the amplified chromosome locus cannot enter the China DNA database, provides a DNA detection kit for auxiliary gender identification for a legal medical expert, or a method for adding auxiliary gender identification to a conventional kit. The key point is that Y special-shaped amplification primer of a fluorescent mark identical to the mark internal standard SIZ mark primer is added on the conventional kit, so as to amplify a segment in SRY zone of the Y chromosome, such as chromosome locus M175 and SRY. Meanwhile, the invention further discloses an SIZ mark primer sequence used for amplifying chromosome locus SRY and M175. Through the improvement, the purpose of auxiliary gender identification can be achieved without adding other components to the kit, or influencing the types of the kit; meanwhile, the amplification segment of Y specificity has the peak at the same fluorescent of the internal standard, peaks of fluorescent marks of other colors are not influenced, the introduction of the data into the public security ministry DNA database is not influenced, thereby conforming to the actual situation of recording for the China public security ministry.

The invention discloses a method for rapidly detecting seed purity of asparagus bean cultivars and a reagent kit thereof, belonging to the field of crop biotechnology and comprising the following steps: (I) downloading a cowpea DNA database, treating sequences and designing SSR primers; (II) calculating polymorphism information content of the SSR primers and screening diagnostic SSR primers; (III) preparing standard fingerprints of five main cultivars of asparagus bean; and (IV) detecting asparagus bean samples to be detected and the like. To facilitate the popularization and application of the method, the invention simultaneously provides a reagent kit which can simultaneously detect hundreds of samples in 4-5 hours and has low detection cost which is about one sixtieth of that of the traditional phenotypic identification, therefore, the invention has the characteristics of rapidness, accuracy, stability and the like, and can be popularized and applied in units for bean research breeding, seed business and the like.

The invention discloses a human STRtyper PCR enlarged fluoroscopic examination reagent kit with quick speed detecting, accuracy, good repeatability, good versatility, low price and practicality. The applicability of the reagent kit for Chinese is remarkable in particular. The serial products of the invention comprise the three following kits: (1) a STRtyper-20G kit with the largest STR gene locusof the global and most powerful recognizing ability; (2) a STRtyper-16GC kit which is more suitably used to build a criminal DNA database of Chinese population and identify the identity; (3) a STRtyper-10F / G kit with brand new STR gene locus. Each kit product comprises the component of 1.1 ml * 1 tube of STR PCR reaction mixture; 0.55 ml * 1 tube of STRtyper primer mixture; 120 microlitre * 1 tube of HS-Taq DNA polymerase; 0.3ml * 1 tube of STR control DNA; 50 microlitre * 1 tube of STRtyper allelomerphic gene mixture; 0.5 ml * 1 tube of internal standard; 30 microlitre* 1 tube of 310 spectrum correcting standard; 30 microlitre * 1 tube of 3100 spectrum correcting standard.

The embodiment of the present invention discloses a high-throughput multi-site SNP detection kit and a detection method thereof. The detection kit includes a primer composition consisting of 188 SNP site-specific primer pairs, and the primer composition The nucleotide sequence is shown in SEQ ID NO: 1-SEQ ID NO: 362; the 5' end of each specific primer is connected with a sample tag sequence, and the 5' of the sample tag sequence of the upstream specific specific primer is The end is connected with a sequencing adapter, and the 5' end of the sample tag sequence of the downstream specific primer is connected with a fixed adapter. The detection kit of the embodiment of the present invention can realize a multi-sample, multi-SNP site parallel and stable test kit. The amount of DNA template required for a single reaction system can be less than 1 nanogram, which is much lower than the amount of sample DNA required for mass spectrometry. One measurement can achieve hundreds of SNP sites for dozens of people. The detection room allows large-scale DNA database construction and use .

The present invention relates to a fluorescence labeled composite amplification detection kit for simultaneously analyzing 17 gene loci of canine genomic DNA. With the system provided by the kit, genetic polymorphisms of the 17 gene loci of the canine genomic DNA can be simultaneously analyzed. The 17 gene loci of the canine genomic DNA comprise DAmel, PEZ1, PEZ2, PEZ3, PEZ5, PEZ6, PEZ8, PEZ12, PEZ15, PEZ20, PEZ21, FH2010, FH2054, FH2079, FH2132, FH2611 and VWFX, is divided into four groups, and relates to the fluorescence labels comprising five colors. The fluorescence labeled composite amplification detection kit provided by the present invention is provided for composite amplification of a mixture comprising oligonucleotide primers of the 17 gene loci. In the system, the gene locus foridentifying the gender of the DNA samples is the DAmel gene locus. The detection results of the system has characteristics of high polymorphism, good balance, high sensitivity, strong specificity, accurate typing result, strong species-specificity, and can fully meet the requirements of dog individual identification, paternity identification, and DNA database construction.

The invention discloses a method for tracing DNA (Deoxyribose Nucleic Acid) of a substance. The method is an addition method and comprises the following steps: designing and synthesizing specific DNA and a confidential primer, and adding the specific DNA at a sample source; carrying out DNA amplification sequencing and tracing on the sample according to the confidential primer; the method comprises the following steps: establishing a specific DNA database D2 of a sample source; and carrying out DNA sequencing and tracing on the sample. According to the method, content anti-counterfeiting can be widely carried out in a low-cost mode, information management anti-counterfeiting traceability is carried out by using a block chain technology, the method is a comprehensive traceability anti-counterfeiting method combining management anti-counterfeiting and technical anti-counterfeiting, and the traceability anti-counterfeiting safety is greatly improved by utilizing the characteristic of high information content of DNA.

The invention discloses an eight-color fluorescent multiplex amplification kit for trace degradation DNA (deoxyribonucleic acid) detection and application. The kit comprises specific amplification primers for amplifying 16 STR (short tandem repeat) gene loci and one sex locus; wherein the 16 gene loci are D7S820, D13S317, CSF1PO, TH01, D2S1338, D16S539, TPOX, D5S818, D19S433, D21S11, D8S1179, vWA, DYS391, D18S51, D3S1358 and FGA, and the sex locus is Amel. Compared with the prior art, the invention has the following advantages: (1) the kit is an eight-color fluorescent multiplex amplification kit, and can accommodate more gene loci within the same fragment length range; (2) the amplification fragments of the gene loci of the kit are smaller than 220bp, so that the kit has a better detection effect on degrading trace detection materials; and (3) the primers in the kit have the advantages of strong specificity, high sensitivity and accurate typing result, and can completely meet the requirements of actual case inspection, DNA database construction and paternity test.

The invention discloses a bovine STR composite amplification detection kit, a primer composition and application thereof. In particular to a primer composition capable of simultaneously amplifying 14 STR (short tandem repeat) loci of cattle, the 14 STR loci are respectively TGLA227, BM2113, ETH10, SPS115, ILSTS006, TGLA126, TGLA122, INRA023, BM1818, ETH225, BM1824, CSRM60, HAUT27 and CSSM66, and the primer composition consists of primers with nucleotide sequences respectively as shown in SEQ ID No.1-28. The primer composition for cattle individual identification and paternity identification, the kit and the detection method have the advantages of high sensitivity, good stability, realization of direct sample amplification, and simple use method, and meet the requirements of cattle DNA related case detection and DNA database construction.

The invention discloses a DNA database building intelligent sorting machine. The machine comprises a chassis, and a DNA information card sorting module arranged in the chassis; the chassis is provided with an inlet for putting in DNA information cards and different outlets for leading out the DNA information cards; and the sorting module can selectively lead out the different types of DNA information cards from the different outlets after receiving the DNA information cards fed from the inlet. The sorting machine can perform full-automatic sorting, needs no manual participation, improves the sorting efficiency, and improves the sorting accuracy rate.

The invention belongs to the technical field of biological detection, and discloses a fluorescence multiplex amplification system of 54 Y chromosome loci, a kit and application. The fluorescence multiplex amplification system comprises a plurality of pairs of specific primers, and can simultaneously amplify 53 Y chromosome STR loci and one Y-Indel. Six-color fluorescence labeling is adopted, the 53 Y chromosomes STR and the one YIndel are subjected to one-tube amplification detection, the requirements of Y-STR DNA database construction of the Ministry of Public Security are met, 20 recommended cores, 15 preferred loci and some alternative loci are included, and high cumulative individual recognition capacity and cumulative non-parent exclusion rate are achieved; and meanwhile, the kit has the characteristics of large information amount and good compatibility, can directly amplify FTA cards, saliva cards and extracted DNA samples, and is simple and convenient to operate.