Human STRtyper PCR amplification fluorescence detection reagent kit
A detection kit and fluorescence-increasing technology, which can be used in measuring devices, microbial measurement/inspection, and material analysis through optical means, and can solve problems such as interference, poor applicability, and high price
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Embodiment 1
[0032] The human STRtyper PCR amplification fluorescence detection kit of this embodiment is STRtyper-20Gkit, which consists of the following components:
[0033] STR PCR reaction mixture 1.1ml×1 tube;
[0034] STRtyper primer mix 0.55ml×1 tube;
[0035] HS-Taq DNA polymerase 120μl×1 tube;
[0036] STR control DNA 0.3ml×1 tube;
[0037] STRtyper allele mixture 50μl×1 tube;
[0038] Internal standard 0.5ml×1 tube;
[0039] 310 spectrum calibration standard 30μl×1 tube;
[0040] 3100 Spectrum Calibration Standard 30μl×1 tube;
[0041] Wherein, the STR PCR reaction mixture is a 5× concentrated solution, and the 5× concentrated solution includes a buffer, and MgCl is dissolved in the buffer 2 and deoxynucleotide triphosphates; the STRtyper primer mixture is a 10× concentrated solution, and the 10× concentrated solution includes fluorescein-labeled and unlabeled primers; the concentration of the HS-Taq DNA polymerase is 5U / μl The concentration of the STR control DNA is 10ng / ...
Embodiment 2
[0095] The human STRtyper PCR amplification fluorescence detection kit of this embodiment is STRtyper-16GCkit, which consists of the following components:
[0096] STR PCR reaction mixture 1.1ml×1 tube;
[0097] STRtyper primer mix 0.55ml×1 tube;
[0098] HS-Taq DNA polymerase 120μl×1 tube;
[0099] STR control DNA 0.3ml×1 tube;
[0100] STRtyper allele mixture 50μl×1 tube;
[0101] Internal standard 0.5ml×1 tube;
[0102] 310 spectrum calibration standard 30μl×1 tube;
[0103] 3100 Spectrum Calibration Standard 30μl×1 tube;
[0104] Wherein, the STR PCR reaction mixture is a 5× concentrated solution, and the 5× concentrated solution includes a buffer, and MgCl is dissolved in the buffer 2 and deoxynucleotide triphosphates; the STRtyper primer mixture is a 10× concentrated solution, and the 10× concentrated solution includes fluorescein-labeled and unlabeled primers; the concentration of the HS-Taq DNA polymerase is 5U / μl The concentration of the STR control DNA is 10ng...
Embodiment 3
[0137] The human STRtyper PCR amplification fluorescence detection kit of this embodiment is STRtyper-10F / Gkit, which consists of the following components:
[0138] STR PCR reaction mixture 1.1ml×1 tube;
[0139] STRtyper primer mix 0.55ml×1 tube;
[0140] HS-Taq DNA polymerase 120μl×1 tube;
[0141] STR control DNA 0.3ml×1 tube;
[0142] STRtyper allele mixture 50μl×1 tube;
[0143] Internal standard 0.5ml×1 tube;
[0144] 310 spectrum calibration standard 30μl×1 tube;
[0145] 3100 Spectrum Calibration Standard 30μl×1 tube;
[0146] Wherein, the STR PCR reaction mixture is a 5× concentrated solution, and the 5× concentrated solution includes a buffer, and MgCl is dissolved in the buffer 2 and deoxynucleotide triphosphates; the STRtyper primer mixture is a 10× concentrated solution, and the 10× concentrated solution includes fluorescein-labeled and unlabeled primers; the concentration of the HS-Taq DNA polymerase is 5U / μl The concentration of the STR control DNA is 10n...
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