The present invention discloses a method for eliminating host
DNA contamination for use in viral metagenomic analysis of
water environment, belongs to the field of
biotechnology, and aims to solve theproblem of a small amount of host cells in a
virus concentrate affect sequencing results in the viral metagenomic research of the
water environment. The method for viral metagenomic analysis of the
water environment is as follows: 1, filtering a
water sample through gauze, removing
bacteria by a filter column of a
filtration system, concentrating, centrifuging, and preparing a
virus particle ultra-speed-
centrifuge concentrate; 2, adding DNase and RNase working solutions, performing PCR amplification, detecting whether
digestion is complete, if the
digestion is not complete, adding
lysozyme tolyse bacterial cells, continuing
digestion, and inactivating
nuclease at high temperature to obtain a
virus particle concentrate without host genetic material
contamination; and 3, amplifying virus whole
genome. The sample is treated by the
lysozyme for release of
DNA of the contaminated bacterial cells, then the
DNA is digested by the
nuclease, and the operation is simple, and the applicabilityis strong.