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Free DNA blood preservative for preventing hemolysis and applications thereof

A technology for preservatives and blood, applied in the field of free DNA blood preservatives, to achieve the effects of simple preparation process, cost reduction, and good protection

Inactive Publication Date: 2019-04-12
苏州依科赛生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report about the free DNA blood preservation agent for preventing hemolysis of the present invention

Method used

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  • Free DNA blood preservative for preventing hemolysis and applications thereof
  • Free DNA blood preservative for preventing hemolysis and applications thereof
  • Free DNA blood preservative for preventing hemolysis and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1 prevents the free DNA blood preservation agent of hemolysis (1)

[0028] This embodiment provides a free DNA blood preservation agent for preventing hemolysis, each 100 ml blood preservation agent includes the following components: 50.0 g of imidazolidinyl urea, 8.0 g of tripotassium EDTA, and 5.0 g of glycine. Preparation method: prepare materials according to the above-mentioned composition ratio, mix imidazolidinyl urea, tripotassium edetate and glycine, add solvent and mix well, set the volume to 100mL, filter and sterilize to obtain the finished blood preservative.

Embodiment 2

[0029] Example 2 prevents the free DNA blood preservation agent of hemolysis (two)

[0030] This embodiment provides a free DNA blood preservation agent for preventing hemolysis, each 100 ml blood preservation agent includes the following components: 40.0 g of imidazolidinyl urea, 20.0 g of tripotassium EDTA, and 10.0 g of glycine. Preparation method: prepare materials according to the above-mentioned composition ratio, mix imidazolidinyl urea, tripotassium edetate and glycine, add solvent and mix well, set the volume to 100mL, filter and sterilize to obtain the finished blood preservative.

Embodiment 3

[0031] Example 3 prevents the free DNA blood preservation agent of hemolysis (three)

[0032] This embodiment provides a free DNA blood preservation agent for preventing hemolysis, each 100 ml blood preservation agent includes the following components: 40.0 g of imidazolidinyl urea, 4.0 g of tripotassium edetate, and 10.0 g of glycine. Preparation method: prepare materials according to the above-mentioned composition ratio, mix imidazolidinyl urea, tripotassium edetate and glycine, add solvent and mix well, set the volume to 100mL, filter and sterilize to obtain the finished blood preservative.

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Abstract

The invention relates to the technical field of blood sample preservation, and particularly discloses a free DNA blood preservative for preventing hemolysis and applications thereof. Each 100ml of theblood preservative comprises the following ingredients: 40.0-100.0g of imidazolidinyl urea, 4.0-20.0g of EDTA-3K, and 4.0-10.0g of glycine. The blood preservative of the invention can reduce the hemolysis phenomenon of blood cells, thereby reducing the release of genomic DNA which can be mixed with free DNA, protecting the short fragments of plasma free DNA from further degradation, preventing the free DNA from cross-linking with proteins, and providing stable environment for the free DNA. The blood preservative for the free DNA of the invention has a good protective effect on the plasma freeDNA, can prevent genomic DNA contamination, and provides high quality samples for downstream free DNA detection.

Description

technical field [0001] The invention relates to the technical field of blood sample preservation, in particular to a free DNA blood preservation agent for preventing hemolysis and its application. Background technique [0002] Cell-free DNA refers to a type of DNA that is free from cells, and the cell-free DNA in the blood of healthy people mainly comes from apoptotic cells. Both tumor cells and pregnant women's fetuses release cell-free DNA into the blood, and these cell-free DNA have become markers for tumor diagnosis and non-invasive prenatal genetic testing. 1mL of human blood contains 1-100ng of free DNA, the content is very low, and in the process of blood sample preservation and free DNA extraction, white blood cells are easy to rupture and release genomic DNA, which affects the quality of free DNA. Ordinary anticoagulant tubes store blood samples, causing blood cells to rupture to release genomic DNA. At present, there are already some blood preservation tubes at h...

Claims

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Application Information

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IPC IPC(8): C12Q1/6806C12M1/24
CPCC12Q1/6806C12Q2527/125
Inventor 韩洪金蒋国成陈旭陈刚
Owner 苏州依科赛生物科技股份有限公司
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