Chinese crab in-vitro hatching technology
A Chinese mitten crab technology, which is applied in the field of Chinese mitten crab nursery, can solve the problems of high cost, high environmental pressure, and low survival rate of crab seedlings, and achieve the advantages of less water consumption, high seedling production rate, and labor saving. Effect
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Embodiment 1
[0018] a. Take the live oviposit crabs after the blastula stage, such as the survival oviposit crabs in the blastula stage, gastrula stage, eye point stage and heartbeat stage;
[0019] b. Turn the ventral surface of the oviposit crab upwards, gently lift the abdomen with hands, and cut off the abdominal limb attached to the egg from the bottom segment with surgical scissors. The abdomen runs through the digestive tract. When cutting the embryos, the opening of the abdomen should be small so that it can be cut; if the opening is too large, cutting too deep will damage the digestive tract and cause parent crabs to die;
[0020] c. Hang it on the metal support (egg hanging device) after being sterilized by permanganate A;
[0021] d. Inflate the embryos for hatching and larvae cultivation, the water temperature is 16.0°C to 25.0°C, the salinity is 30.0 to 34.0, the water is continuously inflated, and the dissolved oxygen is at least 4.5mg / l. When the stage I daphnia (Z 1 ), fee...
Embodiment 2
[0023] Others are the same as in Example 1, the difference is that the cut abdominal limbs are placed in water with a water temperature of 6-8°C and a salinity of 30.0-34.0 for immersion, and the isolated embryos are preserved. The time required by the larvae determines the hatching and cultivation time of the embryo. The effective accumulated temperature of river crabs from fertilization to hatching of larvae is 220°C.d.
Embodiment 3
[0025] Others are the same as in Example 1 or Example 2, except that the cut abdominal limbs are cut into small sections again, and the size of the small sections should be fixed on the support for convenience, so as to increase the contact area between the embryo and the fresh water body. During the hatching of isolated embryos, the water source must be strictly filtered to ensure clean water quality, sufficient dissolved oxygen, and moderate aeration; the temperature difference should not exceed 5°C; Soak the cut embryos with Chinese herbal medicine.
[0026] time
[0027] When the oviposit crabs with the embryos cut off were reared in the cage for 12-15 days, it was found that there were black marks on the cut off abdomen and no inflammatory reaction. Both parent crabs with embryos cut and control crabs with uncut embryos gained 3-2 g in weight. Anatomical observation found that the ovaries of most parent crabs whose eggs were cut off were blue-purple and darker...
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