Freshwater lobster fertilized egg hatching method in vitro

Abstract

The invention discloses an in-vitro hatching method for freshwater lobster zygote. The technical scheme comprises the steps of: putting oogenesis parent lobsters fished in batch into a methylene blue solution to be soaked for 5-15 minutes; lightly stripping zygotes from parent lobster appendages by using sterile tweezers, and putting into a zygote hatcher; and hatching into post larvae after 16-26 days, wherein the hatching rate of the zygote can achieve more than 95%. The method has the advantages of large zygote amount, little occupation land for zygote in-vitro hatching, little water using amount, labors and materials saving, no need of continuous breeding of the oogenesis lobster, and reduction of feeding management cost of the oogenesis lobster. The embryo can survive, and quickly hatched into larvae by adopting the in-vitro hatching method; the size of the larvae is uniform, and the commercial value is high; the mutual killing phenomenon among the larvae can be effectively prevented, and the survival rate of larvae is enhanced; and the in-vitro hatching method is suitable for the in-vitro hatching of scaled freshwater lobster zygotes.

Description

technical field [0001] The invention relates to aquaculture technology, in particular to a freshwater lobster fertilized egg hatching method in vitro. Background technique [0002] The scientific name of freshwater lobster is Procambarus clarkii. Due to its wide adaptability and strong reproductive ability, it can live in rivers, lakes, ponds and paddy fields. Its vitality is extremely strong, and it is suitable for its survival and development in most places in our country. [0003] Due to the rapid growth of market consumption and export processing of freshwater lobster, the number of wild resources is decreasing sharply. Therefore, increasing the production of freshwater lobster through artificial breeding has become one of the important ways to meet the processing export and consumption markets. In recent years, with the sharp increase in market demand, the market price has risen year by year, which has stimulated the enthusiasm of the masses to raise Procambarus clarkii...

AI Technical Summary

Problems solved by technology

This breeding technology simulates the wild environment and it is difficult to form a large scale. The development of larvae is not synchronized, and the size of shrimp larvae is not uniform, resulting in a sharp increase in the probability of larvae killing each other, which seriously affects the survival rate of larvae.