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31results about How to "No sequencing required" patented technology

Method for identifying related species of oyster

The invention relates to a related specie identification technology, in particular to a method for identifying related species of an oyster. The method specifically comprises the following steps of: by using an oyster genome DNA (Deoxyribonucleic Acid) as a template, designing a primer according to a tag sequence of the oyster specie genome; carrying out PCR (Polymerase Chain Reaction) amplification on the primer; and distinguishing oyster species by using Tm value difference in a high-resolution melting curve of an amplification product. Through the adoption of the method provided by the invention, the species can be identified rapidly, economically, simply and conveniently; and compared with a tag sequence sequencing result, an identifying result of the method provided by the invention is 100% in accuracy.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Kit for detecting 7 hot mutant sites of phenylketonuria PAH gene and PCR amplification method thereof

The invention relates to a kit for detecting 7 hot mutant sites of a phenylketonuria PAH gene and a PCR amplification method thereof. The invention is characterized in that in the PCR reaction in which high-fidelity DNA polymerase participates, the product of a mutation detection primer which is modified in a 3'-5' exonuclease digestion resistance way, has very high specificity, and people can directly observe the existence of the strips obtained by gel electrophoresis to judge the genotype of the corresponding mutant sites of the PAH gene. The invention has the advantages of simple operation and economical performance, does not need sequencing, and can be operated in a common molecular biology laboratory. The method of the invention is combined with the fluorescence quantitative PCR technology, and can realize automation and high flux of clinic detection of the biological sample, thereby greatly enhancing the detection accuracy and efficiency.
Owner:SUZHOU KUANGYUAN MOLECULAR BIOTECH

Kit and PCR amplification method for detection of one pathogenic mutation site of male infertility Dmc1 gene

The invention discloses a kit and a PCR amplification method for detection of one pathogenic mutation site of a male infertility Dmc1 gene. The method is characterized in that a modified mutation-detection primer resistant to 3'-5' exonuclease enzyme digestion is used in a PCR reaction in which a high-fidelity DNA polymerase participates, so that the specificity of PCR is improved; and the existing condition of a gel electrophoresis product band can be directly observed to judge the genotype of the Dmc1 gene corresponding mutation site. The detection kit has low requirements on instruments and equipment, is simple in operation, is economic, has no need of sequencing, and can be operated in common molecular biology laboratories.
Owner:GENEHEAL BIOTECH

Specific primers for identifying tortoise shells and identification method thereof

The invention relates to a group of specific primers for identifying tortoise shells and an identification method thereof, and belongs to the technical field of identification of traditional Chinese medicines. Firstly, genomic DNA is extracted and purified after tortoise shell products are crushed, the sample is used as a template, designed and screened specific nucleotide sequences are selected as primers for PCR amplification, then products are subjected to agarose gel electrophoresis analysis and gel imaging system detection. Finally, authenticity and adulteration of tortoise shell productsare judged based on test results. The identification method is easy to operate, does not require sequencing, has strong specificity, strong anti-interference ability, good reproducibility, has a widerange of application, and can be used for accurate identification of tortoise shell origins, medicinal materials and decoction pieces, and a practical method is provided for judging the authenticityand adulteration of the tortoise shell products.
Owner:JIANGSU UNIV

Specific primers and method for identifying stiff silkworms

The invention relates to a group of specific primers and a method for identifying stiff silkworms, belonging to the technical field of identification of traditional Chinese medicines. According to themethod, after a stiff silkworm sample is crushed, the deoxyribonucleic acid (DNA) in domestic silkworms and the DNA in fungi are extracted at the same time by using a CTAB method; the extracted DNA is taken as a template, specific nucleotide sequences, which are designed and screened, are used as primers for performing polymerase chain reaction (PCR) amplification, and the products are then subjected to agarose gel electrophoresis analysis and gel imaging system detection, so that the authenticity and adulteration of the stiff silkworms can be finally detected based on the detection results.The method provided by the invention can be used for judging whether the sample is a stiff silkworm fake product or adulterated product, is simple to operate, does not needing sequencing, is high in specificity, rapid and accurate, and has unique advantages and important application value in the authenticity identification of stiff silkworm samples.
Owner:JIANGSU UNIV

Method for authenticating and distinguishing fusarium culture causing soybean root rot

The invention discloses a method for authenticating and distinguishing fusarium culture causing soybean root rot. The method is characterized in that: fusarium solani and other 3 kinds of fusarium areseparated by using 3 percent agarose gel electrophoresis according to morphology and a genetic evolution tree authentication result built by an ITS sequence, and in terms of an amplified ITS sequencelength; comparing the ITS sequences of the rest 3 kinds of fusarium, finding out a restriction enzyme cutting site BspCNI, AvaI and PstI of a specific-limited incision enzyme; forming different CAPSmolecular markers with ITS sequences obtained by performing enzymatic hydrolysis and amplification on different combinations of the 3 kinds of enzymes, separating the molecular markers with 1.5 percent agarose gel electrophoresis in order to separate the 3 kinds of fusarium. By adopting the method, the authentication and distinguishment of the fusarium culture can be realized by means of the agarose gel electrophoresis and the CAPS molecular markers, the method is suitable for large-scale and high-flux culture authentication and distinguishment, in particular to the rapid authentication and distinguishment of the main fusarium causing soybean root rot in the national soybean production areas.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI

Internal transcribed spacer (ITS2) molecular marker identifying method for aquaculture of catfish hybrids

The invention relates to an ITS2 molecular marker identifying method for aquaculture of catfish hybrids. Southern catfishes, catfishes and hybrids of the southern catfishes and the catfishes serve as objects of study, ITS2 gene sections in catfishes are selected to serve as molecular markers, the length difference the IST2 gene sections of the southern catfishes and the catfishes, and the southern catfishes, the catfishes and hybrids of the southern catfishes and the catfishes are identified successfully. The method has the advantages of being fast in experiment, convenient to operate, visual in identification and free from sequencing.
Owner:TONGWEI

Kit for detecting one pathogenic mutation site in male infertility Galntl5 gene, and PCR amplification method thereof

The present invention discloses a kit for detecting one pathogenic mutation site in male infertility Galntl5 gene, and a PCR amplification method thereof. The method is characterized in that mutation detection primers resisting 3'-5'exonuclease digestion modification are introduced into a high fidelity DNA involved PCR reaction so as to improve the PCR specificity, and the product band of the gel electrophoresis can be directly observed so as to determine the genotype of the corresponding mutation site of the Galntl5 gene. According to the present invention, the detection kit has characteristics of low instrument requirements, simple operation, economy, no requirement of sequencing, and capability of being operated in general molecular biology laboratories.
Owner:GENEHEAL BIOTECH

PCR-HRM primer for quickly detecting salmonella gallinarum and application thereof

The invention discloses a PCR-HRM primer for quickly detecting salmonella gallinarum and application thereof. The PCR-HRM primer for quickly detecting salmonella gallinarum and the application are established for the first time. The PCR-HRM primer is convenient to operate, and during the detection, only saturated fluorescent dye needs to be added into a PCR; the clinical detection process is simplified, the detection time is greatly shortened; and a method has the advantages of being high in throughput and low in cost. True closed tube operation is achieved from the PCR process to HRM analysis, a PCR product does not need to be transferred to other devices in the whole process, cross contamination is avoided, and quantitative analysis can be finished. The PCR-HRM primer is high in specificity, salmonella gallinarum can be distinguished well, and the problem that a conventional detection method is low in salmonella gallinarum identification accuracy is solved. The PCR-HRM primer is high in sensitivity, and the lowest detection limit can be 42 copy numbers per microliter. The PCR-HRM primer has extensive application prospects in rapid diagnosis of salmonella gallinarum.
Owner:SOUTH CHINA AGRI UNIV

Kit for detecting two pathogenic mutation loci in TAB2 gene of human congenital heart disease, and PCR (Polymerase Chain Reaction) amplification method thereof

The invention discloses a kit for detecting two pathogenic mutation loci in a TAB2 gene of a human congenital heart disease, and a PCR (Polymerase Chain Reaction) amplification method thereof. The method is characterized in that a mutation detection primer modified by an anti-3'-5' excision enzyme in an enzyme digestion manner is applied into the PCR in which a high-fidelity DNA (Deoxyribose Nucleic Acid) polymerase is participated, so that the PCR specificity is improved; thus, the genetype of the corresponding mutation loci of the TAB2 gene can be judged by directly observing whether a gel electrophoresis product stripe exists or not. The kit has a low requirement to instruments and equipment, is simple to operate and economic and has no need of sequence measuring. As a result, the kit can be operated in an ordinary molecular biology laboratory.
Owner:GENEHEAL BIOTECH

An SNP marker for identifying Corydalis turtschaninovii Bess., an allele-specific PCR process and application

The invention discloses an SNP marker for identifying Corydalis turtschaninovii Bess., an allele-specific PCR process and application, and relates to the technical field of biology. The SNP marker andthe process are provided based on a problem that there is no specific identification method for Corydalis turtschaninovii Bess.. The invention discloses the SNP marker for identifying Corydalis turtschaninovii Bess., the application of the SNP marker, a specific primer pair T1 for detecting Corydalis turtschaninovii Bess. based on allele PCR detection, and the allele-specific PCR process for Corydalis turtschaninovii Bess. detection. Beneficial effects of the SNP marker, the application, the process, and the specific primer pair are that accurate identification of the Corydalis turtschaninovii Bess. and processing products thereof are achieved, and advantages including high accuracy, high stability, high specificity, no need of sequencing, and no influence caused by medicinal parts are achieved.
Owner:ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE

Kit for detecting male infertility Fkbp6 gene mutation site, and PCR amplification method thereof

The present invention discloses a kit for detecting one pathogenic mutation site in male infertility Fkbp6 gene, and a PCR amplification method thereof. The method is characterized in that mutation detection primers resisting 3'-5'exonuclease digestion modification are introduced into a high fidelity DNA involved PCR reaction so as to improve the PCR specificity, and the product band of the gel electrophoresis can be directly observed so as to determine the genotype of the corresponding mutation site of the Fkbp6 gene. According to the present invention, the detection kit has characteristics of low instrument requirements, simple operation, economy, no requirement of sequencing, and capability of being operated in general molecular biology laboratories.
Owner:GENEHEAL BIOTECH

Kit for detecting one pathogenic mutation site in male infertility Kif18a gene, and PCR amplification method thereof

The present invention discloses a kit for detecting one pathogenic mutation site in male infertility Kif18a gene, and a PCR amplification method thereof. The method is characterized in that mutation detection primers resisting 3'-5'exonuclease digestion modification are introduced into a high fidelity DNA involved PCR reaction so as to improve the PCR specificity, and the product band of the gel electrophoresis can be directly observed so as to determine the genotype of the corresponding mutation site of the Kif18a gene. According to the present invention, the detection kit has characteristics of low instrument requirements, simple operation, economy, no requirement of sequencing, and capability of being operated in general molecular biology laboratories.
Owner:GENEHEAL BIOTECH

Multiple PCR (polymerase chain reaction) detection kit for anopheles peditaeniatus, anopheles lesteri and anopheles barbirostris

ActiveCN111876491ACompleteness is not requiredNon-infectiousMicrobiological testing/measurementAgainst vector-borne diseasesAnopheles barbirostrisAnopheles peditaeniatus
The invention relates to a multiple PCR (polymerase chain reaction) kit for simultaneously detecting anopheles peditaeniatus, anopheles lesteri and anopheles barbirostris. The kit comprises specific primers for the anopheles peditaeniatus, the anopheles lesteri and the anopheles barbirostris, 10 * buffer, dNTPs, MgCl2, Taq enzymes, template DNA and sterile double distilled water, wherein the sequences of the specific primers for the anopheles peditaeniatus, the anopheles lesteri and the anopheles barbirostris are respectively shown as SEQ ID NO.5-6, SEQ ID NO.15-16 and SEQ ID NO.33-34. The multiple PCR detection kit disclosed by the invention can provide a qualitative detection result within 3 hours after a DNA sample is received, saves cost and time, has low requirements for personnel andequipment, and is an effective tool for detecting the anopheles peditaeniatus, the anopheles lesteri and the anopheles barbirostris.
Owner:JIANGSU ACADEMY OF SCI & TECH FOR INSPECTION & QUARANTINE +2

A group of specific primers for identifying turtle shells and its identification method

ActiveCN108018362BAvoid Adsorption LossConcentration is flexibleMicrobiological testing/measurementDNA/RNA fragmentationMedicinal herbsNucleotide
The invention relates to a group of specific primers for identifying turtle shells and an identification method thereof, and belongs to the technical field of identification of traditional Chinese medicines. Firstly, a DNA test sample solution is prepared, and the sample is used as a template, designed and screened specific nucleotide sequences are selected as primers for PCR amplification, then products are subjected to agarose gel electrophoresis analysis and gel imaging system detection. Finally, authenticity and adulteration of turtle shell products are judged based on test results. The method can determine whether the sample is adulterated. The method is easy to operate, does not require sequencing, has strong specificity, strong anti-interference ability, good reproducibility, has awide range of application, and can be used for accurate identification of turtle shell origins, medicinal materials and decoction pieces, and a practical method can be provided for judging the authenticity and adulteration of the turtle shell products.
Owner:JIANGSU UNIV

Molecular detection method and kit for rice photosensitive genic male sterility lines

The invention discloses molecular detection method and kit for rice photosensitive genic male sterility lines. The molecular detection method includes extraction of genome DNA, PCR (polymerase chain reaction) amplification, and enzyme digestion analysis of amplification products. Two pairs of primers and two endonucleases are designed according to a PMS12-1 sequence, and the primers are used for enzyme digestion analysis on the amplification products by PCR so as to determine SNP (single nucleotide polymorphism) type of the photosensitive gene. Therefore, whether the photosensitive genic male sterility gene or fertile gene is contained or not is identified, homozygote and heterozygote of the PMS12-1 gene can be further distinguished, the rice photosensitive genic male sterility lines are identified fast, simply and conveniently, and selection in fields is guided to accelerate breeding process.
Owner:HUNAN HYBRID RICE RES CENT +1

Fast method for detecting gene point mutation of receptor of epidermal growth factor lung cancer in non-cellule type

A method for quickly detecting the gene site mutation of epidermal growth fact receptor for non-small-cell lung cancer includes such steps as providing DNA, PCR amplifying by specially designed three groups of 4 primers, gel electrophoresis, and abserving result.
Owner:SUZHOU KUANGYUAN MOLECULAR BIOTECH

Primer and method for quickly detecting IT15 gene CAG repeat sequence dynamic mutation

The invention provides the primer and method for fast detection of chorea IT15 gene CAG repeat sequence expansion mutation, characterized in that a specially designed primer is employed, and PCR expansion is carried twice to expand DNA amount to gel electrophoresis observable degree, finally gel electrophoresis is utilized to directly observe the situation of IT15 gene CAG repeat sequence dynamic mutation.
Owner:SUZHOU UNIV

A multiplex PCR detection kit for Anopheles claaus, Anopheles confused and Anopheles

The invention relates to a kit for simultaneously detecting Anopheles claaus, Anopheles confused, and Anopheles aedes by multiplex PCR. The kit includes specific primers for An. clau, An. confused, and An. 2 , Taq enzyme, template DNA, sterile double-distilled water, the sequences of the specific primers of Anopheles claau, Anopheles confused, and Anopheles cheesa are respectively as SEQ ID NO.1-2, SEQ ID NO.15- 16, shown in SEQ ID NO.25-26. The multiplex PCR detection kit of the present invention can give qualitative detection results within 3 hours after receiving the DNA sample, saves cost and time, and has low requirements for personnel and equipment. An effective tool for Anopheles mosquitoes.
Owner:JIANGSU ACADEMY OF SCI & TECH FOR INSPECTION & QUARANTINE +2

Microsatellite marker primer pair for identifying genetic sex of brachymystax lenok salmon and sex identification method

The invention discloses a microsatellite marker primer pair for identifying genetic sex of brachymystax lenok and a sex identification method, and relates to a microsatellite marker primer pair for identifying fish sex and a sex identification method. The sequence of an upstream primer F in a primer pair of the microsatellite marker disclosed by the invention is 5 '-CACCACACCGTACCAGTC-3', and the sequence of a downstream primer R in the primer pair is 5 '-TGTGGAGTGGGATG-3'. The sex identification method comprises the following steps: 1, extracting DNA of brachymystax lenok salmon to be identified; 2, carrying out PCR (Polymerase Chain Reaction) reaction; and 3, agarose gel electrophoresis detection. According to the method, the sex is judged through the agarose gel electrophoresis band of the PCR amplification product, only DNA samples need to be extracted, fin rays or scales can be collected, DNA can be extracted, damage to fish bodies is small, fish killing is not needed, and the method can be used for early sex identification.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI +1

Molecular marker evaluation method for wild training panda immune adaptation

ActiveCN112725471AAccurate assessmentRich routine physical examination indicatorsMicrobiological testing/measurementDNA/RNA fragmentationPANDASMedicine
The invention provides a molecular marker evaluation method for wild training panda immune adaptation. The method comprises the following steps of: performing quantitative detection on Marker in a to-be-detected sample through real-time fluorescent quantitative PCR by using congenital immune and adaptive immune genes with remarkable differential expression between wild training pandas and captive pandas with good adaptation as Marker, and evaluating the immune adaptation condition of the wild training panda in the training period from the molecular biology level.
Owner:CHINA CONSERVATION & RES CENT FOR THE GIANT PANDA SICHUAN

Multiple PCR detection kit for Anopheles splendidus, Anopheles maculatus and Anopheles aconitus

ActiveCN111690756ACompleteness is not requiredNon-infectiousMicrobiological testing/measurementAgainst vector-borne diseasesAnopheles aconitusDNA
The invention relates to a kit for simultaneous detection of Anopheles splendidus, Anopheles maculatus and Anopheles aconitus by multiple PCR. The kit includes specific primers of Anopheles splendidus, Anopheles maculatus and Anopheles aconitus, 10xbuffer, dNTPs, MgCl2, Taq enzyme, template DNA and sterile double distilled water, wherein sequences of the specific primers of Anopheles splendidus, Anopheles maculatus and Anopheles aconitus are shown as SEQ ID NO.5-6, SEQ ID NO. 13-14, and SEQ ID NO 25-26. The multiple PCR detection kit can provide qualitative detection results within 3 hours after receiving DNA samples, the cost and time are saved, the requirements on personnel and equipment are low, and the multiple PCR detection kit is an effective tool for detecting Anopheles splendidus,Anopheles maculatus and Anopheles aconitus.
Owner:JIANGSU ACADEMY OF SCI & TECH FOR INSPECTION & QUARANTINE +2
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