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A technical method for identifying whether Bactrocera dorsalis is irradiated or not

A tangerine fruit fly, irradiation technology, applied in biochemical equipment and methods, microbial determination/inspection, device for capturing or killing insects, etc., can solve problems such as unseen research reports

Inactive Publication Date: 2017-04-26
INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of simultaneous irradiation on insect superoxide dismutase (SOD) and acetylcholinesterase (AChE) has not been reported yet.

Method used

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  • A technical method for identifying whether Bactrocera dorsalis is irradiated or not
  • A technical method for identifying whether Bactrocera dorsalis is irradiated or not
  • A technical method for identifying whether Bactrocera dorsalis is irradiated or not

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Effects of Irradiating Bacteralis dorsalis Eggs and Instar Larvae on Phenoloxidase Activity

[0036] The effects of γ-ray irradiation on Bacteralis dorsalis eggs and instar larvae on the phenoloxidase activity were determined, and the results are shown in Table 1, Table 2, and Table 3.

[0037] Table 1 Phenoloxidase (OD 420 value) Enzyme activity

[0038]

[0039] Note: The data in the table are mean ± standard error. Different letters after the data in the same column indicate a difference (DMRT) at the p=0.05 level; the same in the following table.

[0040] It can be seen from Table 1 that the activity of phenol oxidase in the larvae of normal Bactrocera dorsalis increases gradually with age, and the enzyme activity increases significantly on the 4th day of the larvae. The enzyme activity of the 24-hour hatched larvae of the irradiated B. dorsalis eggs was lower than that of the control, and the enzyme activity decreased more significantly with the dos...

Embodiment 2

[0046] Effect of Example 2 on the activity of superoxide dismutase after irradiation of Bactrocera dorsalis eggs and larvae of each age

[0047] The effects of irradiation on the superoxide dismutase activity of Bactrocera dorsalis eggs and instar larvae were measured, and the results are shown in Table 4-6.

[0048] Table 4 The effect of γ-ray irradiation on the eggs of Bacteralis dorsalis for 24 hours on the activity of superoxide dismutase (OD value) in the 2-7 day old larvae

[0049]

[0050] It can be seen from Table 4 that the superoxide dismutase enzyme activity in normal Bactrocera dorsalis larvae gradually increased with age, and the enzyme activity increased significantly on the third day of larvae. The activity of superoxide dismutase in the hatched larvae of Bacteralis dorsalis eggs irradiated for 24 hours was lower than that of the control, and the enzyme activity decreased more significantly with the increase of dose, but the enzyme activity gradually recovere...

Embodiment 3

[0058] Example 3 Effects of Irradiating Bacteralis dorsalis Eggs and Instar Larvae on Acetylcholinesterase Activity

[0059] The effects of irradiation on Bacteralis dorsalis eggs and larvae of various instars on the activity of acetylcholinesterase were measured, and the results are shown in Table 7-9.

[0060] Table 7 Effects of γ-ray irradiation on Bacteralis dorsalis eggs for 24 hours on the activity of acetylcholinesterase (OD value) in 2-7 days old larvae

[0061]

[0062] It can be seen from Table 7 that the activity of acetylcholinesterase in normal Bactrocera dorsalis larvae increases gradually with age, and the enzyme activity of the larvae increases significantly on the 7th day. The changes of acetylcholinesterase activity in the hatched larvae of Bactrocera dorsalis eggs after 24 hours of irradiation were more complicated, and the enzyme activity of the 2nd instar larvae was higher than that of the control. The lower the dose, the higher the OD value of the enzy...

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Abstract

The invention discloses a method for irradiating oriental fruit flies. The method comprises the step that 60 Co gamma rays of dosage ranging from 40 Gy to 160 Gy are used for irradiating oriental fruit eggs, or first instar larvae and second instar larvae; or 60 Co gamma rays of dosage larger than 160 Gy are used for irradiating third instar larvae of oriental fruit flies. The invention further discloses a method for identifying whether oriental fruit flies are processed by irradiation reaching the standard. Live oriental fruit flies on detected plants or fruits are collected to detect the enzymatic activities of phenol oxidase, superoxide dismutase or / and acetylcholine esterase; if the enzymatic activity of the phenol oxidase is smaller than 80% of that of a contrast, the enzymatic activity of the superoxide dismutase is smaller than 85% of that of a contrast, or / and the enzymatic activity of the acetylcholine esterase is smaller than that of a contrast, it is determined that larvae are irradiated. By measuring the enzymatic activities of the phenol oxidase, superoxide dismutase (SOD) or / and acetylcholine esterase (AChE), whether oriental fruit flies are irradiated or not can be determined, and the larval period of the irradiated oriental fruit flies can be determined. The method is quite sensitive and reliable, and only one oriental fruit fly is needed during identification on the basis of the characteristic that the imported fruit intercept insect quantity is small.

Description

technical field [0001] The invention relates to a method for identifying whether Bactrocera dorsalis is irradiated or not, belongs to the field of plant pest detection, and specifically relates to inspection and quarantine technology. Background technique [0002] Irradiation quarantine technology achieves the purpose of quarantine by killing pests or preventing eggs or larvae of quarantine pests from emerging. Fresh fruits and vegetables are likely to be infested with egg or larval pests prior to irradiation. Irradiation at recommended quarantine doses can meet the quarantine requirements for fresh fruits or vegetables, but does not immediately kill quarantine pests (egg or larval form), so it is likely that non-reproductive live quarantine larvae will be found in irradiated quarantine-treated products . Since the 1990s, people have been developing methods to identify larvae in irradiated products that have indeed been irradiated, to ensure the effectiveness of irradiatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01M1/22C12Q1/46C12Q1/26
Inventor 赵菊鹏武目涛赵立荣梁帆马骏梁广勤
Owner INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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