47results about How to "Strong binding specificity" patented technology

The invention relates to the technical field of biological engineering. The B structure domain gene monomer of protein A is optimized according to the preference of a colon bacillus colon, six series bodies are constructed and inserted into a thermally induced colon bacillus vector pBV220, and a colon bacillus expression vector containing the gene and a converted colon bacillus DH5alpha recombination strain thereof are constructed. And a method for producing the recombination protein A using the strain is also provided. The total soluble bacterial protein amount of the soluble recombination protein A produced by the strain can be more than 80 percent, and the recombination protein A can be purified to over 95% just by subsequent nickel ion chelate chromatography and molecular sieve column chromatography. Moreover, the protein has the advantages of high expression amount, low cost, easy purification and the like. A recombination protein A affinity stuffing prepared by using the protein has the advantages of high human IgG absorption capacity, low proA dropping and the like. The invention provides a practical and feasible approach to obtain the recombination protein A with high quality and low price as an antibody (medicine) purifying medium.

The present invention relates to a mouse anti-human CD123 monoclonal antibody and application thereof, comprising a heavy chain variable region sequence and a light chain variable region sequence; the CDR region of the heavy chain variable region sequence comprises three sequences as follows, respectively As shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3; the CDR region of the light chain variable region sequence comprises three following sequences, respectively as SEQ ID NO: 4, SEQ ID NO : 5, shown in SEQ ID NO: 6. The anti-CD123 monoclonal antibody 13C3 provided by the present invention can not only be used to detect cells expressing human CD123, but also can be used alone or in combination with other methods in tumor immunotherapy targeting human CD123 protein, that is, it can be effectively used in the treatment of tumors, infections In the preparation of drugs for sexually transmitted diseases, autoimmune diseases and anti-immune rejection.

The invention discloses a staphylococcus protein A, a purification preparation method and application thereof. The invention mainly relates to the construction of a recombinant plasmid by linking a genetically engineered protein ProteinA gene into an expression vector, and then introducing it into a prokaryotic host cell for expression. And and ion exchange chromatography purification, can obtain the target protein with a purity of more than 95%. The purification preparation method of the invention has the characteristics of simple purification process and high yield. At the same time, the prepared ProteinA has good immunoglobulin IgG binding ability, alkali resistance and thermal stability. In addition, the affinity medium prepared by the solid-phase carrier coupled with ProteinA has the advantages of good IgG adsorption effect, high sample loading capacity and alkali resistance. The ProteinA protein prepared by the invention and the medium prepared by coupling can play an important role in the field of monoclonal antibody purification and immunodiagnosis research.