38results about How to "Screened" patented technology

The invention provides a method for preparing carbon-supported nucleocapsid type Ni-Pt particles for direct methanol fuel cell catalysts, which belongs to a preparation process of direct methanol fuel cell catalysts. The method comprises the steps of adopting sodium citrate as a stabilizer, adopting cationic surfactant CTAB as dispersant, using sodium hypophosphite to reduce nickel acetate, generating a Ni kernel on the surface of Vulcan XC-72 or mesoporous carbon treated with sodium borohydride, washing superfluous sodium hypophosphite and generating a Pt shell on the surface of the Ni kernel through chemical replacement. The catalyst has a structure with the Ni kernel and the Pt shell, and has the advantages of low Pt support amount and high catalytic activity.

The invention relates to the technical field of genetic engineering antibody and provides a monoclonal antibody. The variable region of heavy chain of the monoclonal antibody contains the amino acid sequences shown in SEQ ID NO.1, SEQ ID NO.2 and SEO ID NO.3; the variable region of light chain of the monoclonal antibody contains the amino acid sequences shown in SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6. The invention also specifically provides a humanization single-chain antibody generated by the recombinant strain of enterotoxigenic Escherichia coli with the accession number CGMCC No.2821 and the amino acid sequence of the humanization single-chain antibody is shown in SEQ ID NO.7. The antibody of the invention can be specifically bound with the A-beta oligomer, effectively inhibit the fibrosis aggregation of A-beta and obviously alleviate the toxic effect of the A-beta on cells. The invention also relates to a pharmaceutical composite containing the antibody. The antibody of the invention has strong activity, good specificity, easy preparation and wide prospect of experiment application and clinical application.

The invention discloses an advanced photoresist process quality evaluation method and system, wherein the advanced photoresist process quality evaluation system comprises an image acquisition module,a contour extraction module, a roughness calculation module, a fitting module, a feature analysis module and a quality evaluation module, wherein the image acquisition module, the contour extraction module, the roughness calculation module, the fitting module, the feature analysis module and the quality evaluation module are connected in sequence to photoetch the acquired photoresist to obtain anelectron beam microscopic image; according to the electron beam microscopic image, extracting the photoresist edge contour, and calculating the edge roughness and the width roughness at the same time;and calculating a power spectral density curve by utilizing a fitting function, obtaining fitting parameters, calculating characteristic parameters of the electron beam microscopic image, and establishing a photoresist quality evaluation table according to the characteristic value of the photoresist, so that an optimal photoresist product can be screened.

The invention relates to a method for repaid rejuvenation of a flammulina velutipes degraded strain. The method includes steps: degraded strain transferring, fruiting body induction, rejuvenated strain acquisition and rejuvenated strain detection. The rejuvenation operation is performed on the flammulina velutipes degraded strain, an obtained rejuvenated strain is subjected to cultivation comparison with the degraded strain, and the rejuvenated strain which recovers original agronomic characters or is superior in character is a successfully rejuvenated flammulina velutipes strain. The rejuvenation method is provided for the flammulina velutipes degraded strain and is convenient and simple in operation, short in rejuvenation period, low in cost, less prone to contamination and applicable torapid purification and rejuvenation of the flammulina velutipes degraded strain, and the rejuvenated strain has excellent characters.

The invention belongs to the field of gene engineering and clinical medicine and particularly relates to an endemic arsenism early diagnosis associated serum exosome miRNAs marker and application thereof. The marker is a combination of has-miR-155 and has-miR-191. The marker and a primer thereof can be used for preparation of a kit for early diagnosis of endemic arsenism.

The invention relates to high frequency low loss microcrystalline glass containing lanthanum oxide and a preparation method of the microcrystalline glass. The microcrystalline glass comprises the following components in percentage by weight: 6-9% of MgO, 19-27% of Al2O3, 26-37% of SiO2, 15-21% of TiO2, 0-7% of CeO2 and 7-32% of La2O3. According to the microcrystalline glass provided by the invention, CeO2 is replaced by La2O3, La and Ce are in adjacent positions in the periodic table and are greatly similar, and meanwhile, La<3+> ions are colorless and valence is not changed, so that the transparency of original glass is improved under the condition that the forming capacity and performance of the glass are not subjected to relatively great influence, defected products with defects in the founding process are easily screened, the batch rate is improved and the production cost is lowered.

The invention belongs to the field of gene engineering and clinical medicine and particularly relates to an endemic arsenism early diagnosis associated serum exosome miRNAs marker and application thereof. The marker is a combination of has-miR-155 and has-miR-191. The marker and a primer thereof can be used for preparation of a kit for early diagnosis of endemic arsenism.

The invention discloses a method for obtaining transgenic cotton by transforming live immature embryos mediated by Agrobacterium, which consists of the steps of peeling off aseptic embryos, preparing transformation liquid, shaking and infecting, preparing transgenic cotton, and identifying transgenic plants. In the aseptic embryo stripping, 30% hydrogen peroxide and sterile water are prepared into a disinfectant solution, the cotton seeds are plucked and put into the disinfectant solution, soaked at 25°C for 24 hours, the seeds are taken out and placed in a petri dish, and the seed coat and 1 / 3 to 1 / 2 cotyledons, and the remaining cotton embryos are placed in a petri dish containing basal medium to injure the cotton embryos; in the step of preparing the transformation solution, the induction solution is made of acetosyringone and magnesium sulfate, and acetosyringone Ketones can induce the activation of the Vir gene of Agrobacterium and improve the efficiency of exogenous gene integration; in the shaking infection step, Silwet L‑77 and MS liquid medium were added to the transformation solution to make Agrobacterium cover cotton embryos in a wider range. The invention has the advantages of easy screening, short cotton growth cycle, easy operation, transformation efficiency and the like.

The invention discloses a composition for digesting allelochemicals in semi-substrate covering raw materials and a using method of the composition, and belongs to the field of garden engineering. Thecomposition comprises ground bark particles and a broad-spectrum microorganism combined composting substance. The using method comprises the following steps: S1, nutrient soil is laid on a bottom layer, and plant fibers, a nutrient substance and an original bacterium solution are sequentially added on the surface of the nutrient soil from top to bottom; S2, operators gather the nutrient soil and the composition into a long strip-shaped gathering pile with a width of 1-2.5 m and a height of 1.5-2 m, meanwhile, air-permeable coverage is carried out on the surface of the gathered object, and semi-corrosion treatment is carried out; S3, the operators control the semi-corrosion treatment temperature to be 43-52 DEG C; and S4, the operators replace the surface of the gathered object by a closedcover, decomposition treatment is carried out, and the temperature is controlled to be 60-65 DEG C in the decomposition process. According to the technical scheme, broad-spectrum microorganisms are used for decomposition, so that tree bark is prevented from generating an uncoupling agent, and the damage to plant roots is avoided.

The invention relates to an electrode assembly, a lithium-oxygen thin-film battery and a preparation method thereof. The electrode assembly comprises an insulating substrate, a positive electrode filmlayer, a negative electrode film layer and a protective layer. The positive electrode film layer and the negative electrode film layer are located on the same surface of the insulating substrate, a space is between the positive electrode film layer and the negative electrode film layer, and the protective layer is located on the upper surface of the negative electrode film layer. The electrode assembly has a simple structure, provides a reliable platform for in-situ characterization, and has the advantages of large size adjustable range, high material utilization, and simple and rapid preparation method. The lithium-oxide thin-film battery prepared from the electrode assembly facilitates integrated design and battery portable processing.

The present invention relates to the technical field of genetically engineered antibodies, and provides a monoclonal antibody whose heavy chain variable region contains the amino acid sequences shown in SEQ ID NO.1, SEQ ID NO.2 and SEO ID NO.3, and the light chain variable region The region contains the amino acid sequences shown in SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6. The present invention also specifically provides a humanized single-chain antibody produced by a genetically engineered strain of Escherichia coli with a deposit number of CGMCC No. 2821, and its amino acid sequence is shown in SEQ ID NO.7. The antibody of the invention can specifically bind to the A-beta oligomer, effectively inhibit the fibrosis aggregation of A-beta, and obviously reduce the cytotoxic effect of A-beta on cells. The invention also relates to a pharmaceutical composition containing the antibody. The antibody of the invention has strong activity, good specificity, is easy to prepare, and has broad experimental application and clinical application prospects.

The invention belongs to the field of plant genetic engineering, and particularly relates to a tobacco NtMLO1 and NtMLO2 fixed-point co-knockout system and application thereof. The invention discloses a homologous sequence of tobacco genes NtMLO1 and NtMLO2, a target sequence is designed based on the homologous sequence, a tobacco NtMLO1 and NtMLO2 fixed-point co-knockout system is constructed, the tobacco NtMLO1 and NtMLO2 genes can be knocked out at the same time, and the simultaneous mutation efficiency of the two genes is 50.0%. The invention provides an efficient breeding mode for creating powdery mildew resistant germplasm resources of tobacco and cultivating powdery mildew resistant tobacco varieties.

The invention relates to the technical field of dairy products, and discloses a method for screening flavored milk powder formulas. The method of the present invention simulates the production process to design the rapid viscosity analyzer (RVA) measuring program of the common modulated milk powder formula, and tests the viscosity change of the common modulated milk powder feed liquid in the simulated production heating process; simultaneously monitors the actual production with the same formula product The viscosity of the thick milk before spraying at the control point was compared, and the consistency of the viscosity data confirmed that the method was feasible, so as to determine the RVA measurement procedure of the flavored milk powder, and then screen the flavored milk powder formula according to the characteristic value of the viscosity curve. The method of the invention is easy to operate, and the characteristic value of the viscosity curve is clear. As the basis for judging the flavored milk powder formula, it is concise and clear, easy to judge, and can quickly screen the formula. And it effectively reduces the impact caused by the deviation of production experience, reduces the unfavorable loss caused by trial production, and reduces the cost and time of research and development of new flavored milk powder.

The invention discloses a semi-finished product of an O-shaped ring with an inner diameter smaller than 0.5 mm, a forming die and a trimming method thereof, and belongs to the field of trimming of rubber products. The semi-finished product of the O-shaped ring with the inner diameter smaller than 0.5 mm comprises the O-shaped ring, wherein a lug boss is arranged on the cutting edge of the inner circle of the O-shaped ring; a diamond-shaped cutting edge is arranged on the cutting edge of the outer circle of the O-shaped ring; the diamond-shaped cutting edges of the outer circles of adjacent O-shaped rings are overlapped with each other, so that the semi-finished products are connected to form a netlike structure. The cavity of the forming die corresponds to the structure of the semi-finished product of the O-shaped ring. The trimming method comprises the steps of: (1) charging; (2) spraying a lubricant; (3) trimming centrifugally; (4) sorting. The method realizes the purpose of reducing trimming length of the O-shaped ring with the inner diameter smaller than 0.5 mm, improving the sealing performance of the O-shaped ring, and reducing the dynamic sealing friction resistance.

The invention provides a method and device for identifying electronic manuscripts. The trigger circuit makes the nib heat, so that the temperature of the nib is significantly higher than the ambient temperature; when the nib touches an object, the effective contact information is sent to the infrared camera through wireless transmission; The infrared video image is sent to the recognition processing circuit to correct the infrared video image in turn and record the position of the pen tip in each frame of the infrared video image after correction, and then convert it into two-dimensional coordinates for recording; complete the contact at the pen tip Afterwards, the infrared camera stops shooting, and the recognition processing circuit linearly connects the nib coordinate points of all image frames in this contact process according to the sequence of image acquisition to form a manuscript handwriting image and stores it, and simultaneously performs text recognition processing and caches it. The invention can combine the image character recognition technology and the writing tool to record the original manuscript and transform the electronic characters while writing.

The invention relates to the technical field of engineered antibody and provides a monoclonal antibody. The variable region of heavy chain of the monoclonal antibody contains the amino acid sequences shown by SEQ ID NO.1, SEQ ID NO.2 and SEO ID NO.3; the variable region of light chain of the monoclonal antibody contains the amino acid sequences shown by SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6. The invention also specifically provides a humanization single-chain antibody generated by the recombinant strain of enterotoxigenic Escherichia coli with the accession number CGMCC No.2819 and the amino acid sequence of the humanization single-chain antibody is shown in SEQ ID NO.7. The antibody of the invention can be specifically bound with the A-beta oligomer, effectively inhibit the fibrosis aggregation of A-beta and obviously alleviate the toxic effect of the A-beta on cells. The invention also relates to a pharmaceutical composite containing the antibody. The antibody of the invention has strong activity, good specificity, easy preparation and wide prospect of experiment application and clinical application.

The invention discloses a double-cross yeast gER beta 1-GRIP1 for reorganizing an estrogen receptor of gobiocypris rarus and application of the double-cross yeast gER beta 1-GRIP1. The invention discloses double-cross yeast. The double-cross yeast is obtained by transferring a coding gene of fusion protein of a ligand binding region and a DNA bonding structure region containing the estrogen receptor beta 1 of the gobiocypris rarus and a coding gene of fusion protein of a common activity factor and a copying activity structure region containing the estrogen receptor into fluorescent yeast of which the gene type is MAT alpha, ura 3-5, ade 2-101 trp 1-901, leu2-3, 112, gal4 delta, met-, gal80 delta, URA:: GAL1UAS-GAL1TATA-Luc. The double-cross yeast gER beta 1-GRIP1 has important application in detecting the toxic equivalent or the size of potential ecological toxicity in natural estrogen and / or similar estrogen compounds in environmental samples, in particular assessing the risk which may exist in the endocrine system of the gobiocypris rarus.

The application discloses an information correction method, device, electronic equipment and storage medium, and relates to speech recognition technology. The specific implementation plan is: obtain the speech recognition result matching the interactive speech currently input by the user, and perform entity recognition in the speech recognition result to obtain entity feature information; according to the latest acquired historical speech recognition result, and the speech As a result of the recognition, contextual information is generated; according to the contextual information and the entity feature information, clarification and correction information matching the historical speech recognition results is obtained. The embodiment of the present application realizes that only by inputting the voice, the word or word that was incorrectly recognized in the last historical voice recognition result due to a slip of the tongue or homonym problems can be modified, which avoids the user's manual input and modification, improves the modification efficiency, and makes the voice interaction process more natural.