The invention belongs to the technical field of
gene engineering, and particularly relates to a transposon carrier and
system for preparing an immortalized
cell and a usage method thereof. The transposon carrier and
system for preparing the immortalized
cell and the usage method thereof aim at solving the technical problem of providing a new choice for preparing the immortalized
cell. According to the technical scheme, the
piggyBac transposon carrier for preparing the immortalized cell contains an SV40TAg, the two ends of the SV40TAg are provided with FRT sites, and the two ends of a fragment (hygromycin-SV40TAg or
neomycin-SV40TAg) which is actually inserted into a
genome contain piggyBac
transposase splice sites. The invention further provides a host cell containing the carrier and the
system for preparing the immortalized cell, and the system comprises the carrier and a piggyBac
transposase expression carrier. By adopting the carrier, an immortalized
cell system can be established more effectively and quickly.