30 results about "Genetic function" patented technology

The present invention provides a complex comprising an antibody that targets an exosome surface antigen or an antibody fragment of the antibody and a suppressor of a gene or an expression product of the gene, wherein the antibody or the antibody fragment and the suppressor of the gene or the expression product of the gene are covalently bound to each other directly or through a linker or are non-covalently bound to each other.

The invention discloses a method of completely acquiring ribosomal nascent peptide chain compound from plant tissues and application of the method. The method includes the steps of washing fresh plant tissues with water, quickly freezing the fresh plant tissues to -80 DEG C with liquid nitrogen, and extracting RNC (ribosome nascent-chain complex); or storing the plant tissues, quickly frozen by the liquid nitrogen, at the temperature not higher than -80 DEG C, and after stable storage and transport at such temperature, and extracting the RNC. The step of extracting the RNC includes adding the liquid nitrogen to grind the plant tissues to obtain powder; adding pre-cooled cell lysis buffer into the powder in the liquid nitrogen environment, and performing grinding and mixing; placing mixture on ice, and allowing lytic reaction after the ice thaws; performing centrifuging, and extracting the RNC form supernate. The method has the advantages that the RNC in the plant tissue sample can be conveniently and effectively acquired, the requirement of the RNC sample for long-term storage and transport is met, and analysis on the genetic functions is facilitated.

The invention belongs to the technical field of genetic engineering and discloses a rice leaf senescence control gene OsCKX11 and its application. The amino acid sequence of the rice leaf senescence control gene OsCKX11 has at least 70% homology with the amino acid sequence shown in the amino acid sequence SEQ ID No.1 sex. The present invention conducts site-directed mutation of rice cytokinin oxidase coding gene OsCKX11, analyzes the phenotypic changes of its mutants, and finds that the lack of function of this gene will cause late senescence of rice leaves; in some premature senescence cultivars, OsCKX11 is knocked out by genetic engineering technology It can purposely regulate the aging process of plants and adjust the development period of plant populations to achieve the purpose of increasing production, which has very important application value.

The invention discloses a function and an application of a Nulp1 (Nuclear localized protein-1) in treatment of myocardial hypertrophy and belongs to the field of genetic functions and applications. The correlation between Nulp1 expression and myocardial hypertrophy is determined, research results show that Nulp1 expression is reduced remarkably compared with a normal group in a model with myocardial hypertrophy; myocardial hypertrophy and fibrosis are promoted remarkably and cardiac functions are deteriorated when the Nulp1 expression is inhibited, and myocardial hypertrophy and fibrosis are inhibited remarkably and the cardiac functions are protected when Nulp1 overexpression is promoted. Therefore, the Nulp1 can be taken as a target gene, is used for screening drugs for protecting the cardiac functions, resisting cardiac fibrosis and / or preventing, relieving and / or treating myocardial hypertrophy and is used for preparing drugs for protecting the cardiac functions, resisting myocardial hypertrophy and / or preventing, relieving and / or treating myocardial hypertrophy, and a new effective way for treatment of myocardial hypertrophy is provided.

The invention relates to the field of wheat genetics and its molecular breeding, in particular to the development and application of functional markers of the powdery mildew resistance gene of the goat grass. The present invention is effective in anti-powdery mildew gene of goat grass Ml2147 On the basis of map-based cloning, using the specific structural sequence of its candidate gene, developed SDAU‑2147 Functional markers and verification using more than 180 natural germplasms of A. tachyphylla. The results prove that the functional marker provided by the present invention has the characteristics of fast, direct, high-throughput, etc., and has high specificity. Worked on wheat powdery mildew resistance breeding.

The invention provides a novel method for inhibiting arabidopsis thaliana living nutritional type oomycete downy mildew infection based on small RNAs, and belongs to the technical field of biology. Cellulose synthase A3 of arabidopsis thaliana living nutritional type oomycete is taken as a target gene to synthesize antisense sRNAs with the length >=25nt, and the germination of arabidopsis thalianaliving nutritional type oomycete spores is inhibited through the antisense sRNAs to inhibit the arabidopsis thaliana living nutritional type oomycete downy mildew infection. The method has the advantages of reliability and effectiveness, and it is indicated that a simple and effective sRNA method has potentials on deciphering genetic functions in specialized biotrophic pathogens and the controlling of plant diseases.

The invention discloses a genetic manipulation system based on Hfx. mediterranei and pyrF genes and its application. The genetic manipulation system comprises recombinant halophilic archaea and a recombinant plasmid carrier. The recombinant halophilic archaea is prepared by defunctionalizing an encoding gene of a protein sequence of SEQ ID NO:1 in initial halophilic archaea. The recombinant plasmid carrier at least contains a replication origin required by replication of Escherichia coli, an expression cassette for screening resistance selectable marker genes of Escherichia coli transformants, an expression cassette of the encoding gene of the protein sequence of SEQ ID NO:1, and polycloning sites for allowing exogenous genes to insert. The genetic manipulation system can be used as a high-efficiency gene knockout system, and can carry out extensive genetic function research and metabolic pathway illumination on Hfx. mediterranei. According to the experiment, the frequency of positive recombinants obtained from genetic transformation by using the inventive system is greatly improved.

The invention discloses a genetic manipulation system based on Haloarcula hispanica and pyrF genes and its application. The genetic manipulation system comprises recombinant halophilic archaea and a recombinant plasmid carrier. The recombinant halophilic archaea is prepared by defunctionalizing an encoding gene of a protein sequence of SEQ ID NO:1 in initial halophilic archaea. The recombinant plasmid carrier at least contains a replication origin required by replication of Escherichia coli, an expression cassette for screening resistance selectable marker genes of Escherichia coli transformants, an expression cassette of the encoding gene of the protein sequence of SEQ ID NO:1, and polycloning sites for allowing exogenous genes to insert. The genetic manipulation system can be used as a high-efficiency gene knockout system, and can carry out extensive genetic function research and metabolic pathway illumination on Haloarcula hispanica. According to the experiment, the frequency of positive recombinants obtained from genetic transformation by using the inventive system is greatly improved.