Agrobacterium-mediated dendrocalamus latiflorus transformation method

Abstract

The invention discloses an agrobacterium-mediated dendrocalamus latiflorus transformation method which comprises the steps of preparing explants, preparing an agrobacterium infected bacteria solution, infecting, screening, inducing of adventive buds, and inducting and rooting. agrobacterium is utilized to infect explant at stem apex of dendrocalamus latiflorus, co-culture is performed in a NB culture medium containing 2 mg / L, 2,4-D and 200 mu M acetosyringone, and steps such as screening are performed to finally obtain transgenic dendrocalamus latiflorus. The agrobacterium-mediated dendrocalamus latiflorus transformation method utilizes an agrobacterium-mediated efficient transformation system to firstly establish a transgenic system, which takes dendrocalamus latiflorus nutritive organs as start, of dendrocalamus latiflorus, and the system can overcome the obstacle that the conventional breeding method cannot be applied to bamboo, and provides premise for molecular breeding of dendrocalamus latiflorus through the transgenic technology. Through the method, technical support is provided for researching genetic functions of bamboos in the basic research field.

Description

technical field [0001] The invention belongs to the field of forest tree genetics and breeding, and in particular relates to an Agrobacterium-mediated transformation method of bamboo. Background technique [0002] Bamboo is a subgenus of Bamboo, Bamboo subfamily of Poaceae. It is naturally distributed in Fujian, Taiwan, Guangdong, Guangxi, Guizhou, Yunnan and other parts. High economic value and social value. [0003] With the development of social economy, people have higher and higher requirements on the output and quality of bamboo, but correspondingly, the bamboo breeding lags far behind. Traditional genetic breeding methods cannot be carried out in bamboo. The main reason is that the flowering of bamboo is long-term and uncertain, and hybridization between bamboo species cannot be carried out. Transgenic breeding is an important means in crop breeding. Precise gene regulation through transgenic can greatly improve the yield and quality of bamboo. [0004] At present,...

AI Technical Summary

Problems solved by technology

The present invention utilizes this method to solve the long-standing problem of difficulty in gene transfer of Mazak bamboo, and can be widely used in functional gene identification and molecular breeding of Mazak bamboo

Images