Mouse anti-Aspergillus polysaccharide hybridoma cell line, monoclonal antibody and application

A hybridoma cell line, monoclonal antibody technology, applied in applications, anti-fungal/algae/lichen immunoglobulin, instruments, etc., can solve the problem of low culture positive rate and achieve the effect of strong binding specificity

Active Publication Date: 2022-03-01
TIANJIN ERA BIOLOGY TECH CO LTD +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the widespread use of a large number of broad-spectrum antibiotics, the culture-positive rate is also very low

Method used

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  • Mouse anti-Aspergillus polysaccharide hybridoma cell line, monoclonal antibody and application
  • Mouse anti-Aspergillus polysaccharide hybridoma cell line, monoclonal antibody and application
  • Mouse anti-Aspergillus polysaccharide hybridoma cell line, monoclonal antibody and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] An embodiment of the present invention will be described below in conjunction with the accompanying drawings.

[0035] The invention relates to a mouse-resistant Aspergillus polysaccharide hybridoma cell strain. The biological material is named 3G3, which belongs to hybridoma cells. The microorganism preservation number is CGMCC No.16813. Dated November 22, 2018, tested as alive.

[0036] 1 mouse anti-Aspergillus polysaccharide monoclonal antibody

[0037] The mouse anti-Aspergillus polysaccharide monoclonal antibody produced by the mouse anti-Aspergillus polysaccharide hybridoma cell line comprises a heavy chain variable region sequence and a light chain variable region sequence; wherein, the CDR region of the heavy chain variable region sequence contains three sequences, which are:

[0038] SEQ ID NO: 1 GFDFSRYWMS (CDRH1)

[0039] SEQ ID NO:2 EINPDSSTINYTPSLKD(CDRH2)

[0040] SEQ ID NO: 3 NSYGTLDY (CDRH3)

[0041] The CDR region of the light chain variable region...

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Abstract

The invention provides a mouse anti-aspergillus polysaccharide hybridoma cell line, aspergillus polysaccharide monoclonal antibody and the application in the kit. The beneficial effect of the present invention is: the present invention clones the Ig variable region gene through mouse hybridoma monoclonal antibody screening and RT-PCR method, obtains the hybridoma and its variable region sequence of stably secreting anti-Aspergillus polysaccharide antibody, and uses ELISA The binding specificity of the antibody was identified by this method, which laid the foundation for the research and development of anti-Aspergillus polysaccharide genetically engineered antibody; Aspergillus polysaccharide detection.

Description

technical field [0001] The invention belongs to the field of antibody preparation and sequence determination, and in particular relates to a mouse anti-Aspergillus polysaccharide hybridoma cell line, monoclonal antibody and application. Background technique [0002] Diseases caused by fungal infections, especially deep fungal infections, are called mycoses. There are many types of fungi in nature, and compared with bacteria, there are relatively few people who are pathogenic to humans. However, in recent years, due to the large number of clinical applications of broad-spectrum antibiotics, adrenal cortex hormones and immunosuppressants, fungal infections have a clear increase trend. At present, the basic methods for the diagnosis of fungal diseases include: clinical manifestations, mycology, immunology and pathology. The most direct way is to be able to demonstrate the presence of the fungus in the tissue, or in the exudate, which can be isolated and cultured. However, du...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20C12N15/13C07K16/44C07K16/14G01N33/577G01N33/569C12R1/91
CPCG01N33/56961G01N33/577C07K16/14C07K16/44C07K2317/565C07K2317/56C07K2317/33
Inventor 苑庆华何永胜张桐嘉樊琳琳王玉芳臧丹戎黄炎彬王兴
Owner TIANJIN ERA BIOLOGY TECH CO LTD
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