Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cell model for screening developmental toxicity exogenous compounds by taking 11beta-HSD2 as target, as well as construction method and application of cell model

A cell model and compound technology, applied in the field of drug toxicology, can solve the problems of lack of mechanism theory system, difficult cultivation, poor specificity, etc., and achieve the effect of mature extraction and identification methods, simple growth environment, and strong binding specificity

Active Publication Date: 2020-03-24
WUHAN UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current evaluation methods use the results of the overall animal reproduction experiment as a measure of the developmental toxicity of exogenous compounds, but there are disadvantages such as large amount of animals used, long experimental period, poor specificity, low sensitivity, and the inability to elucidate its mechanism from the cellular or molecular level.
Even the developmental toxicity in vitro evaluation system gradually developed in recent years, such as whole embryo culture, micromass culture method and embryonic stem cell culture, still has problems such as difficult culture, high cost, limited time or low specificity of detection indicators
To sum up, the in vitro evaluation system for the developmental toxicity of exogenous compounds has great limitations, which are mainly related to the unclear performance of the developmental toxicity of exogenous compounds and the lack of a theoretical system for the mechanism of occurrence. Therefore, high-throughput developmental toxicity of exogenous compounds based on toxicity mechanisms and molecular targets In vitro evaluation system is yet to be developed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell model for screening developmental toxicity exogenous compounds by taking 11beta-HSD2 as target, as well as construction method and application of cell model
  • Cell model for screening developmental toxicity exogenous compounds by taking 11beta-HSD2 as target, as well as construction method and application of cell model
  • Cell model for screening developmental toxicity exogenous compounds by taking 11beta-HSD2 as target, as well as construction method and application of cell model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] [Example 1] Construction of a cell model for screening developmental toxic exogenous compounds with 11β-HSD2 as the target

[0044] 1. WJ-MSCs acquisition

[0045] The research involving human specimens in the present invention strictly follows the "Declaration of Helsinki of the World Medical Association". The collection of umbilical cord tissue has obtained the informed consent of the newborn's parents and signed a paper version of the informed consent, and the experimental method has passed the approval of the relevant ethics committee. The umbilical cords of cesarean section fetuses were taken under aseptic conditions in the operating room, washed with blood, soaked in pre-cooled saline and brought back to the laboratory.

[0046] 2. WJ-MSCs extraction

[0047] The umbilical cord was taken out in the ultra-clean workbench, and all separation processes were performed in pre-cooled saline. Cut into 4-6cm / section, and wash away the blood with a syringe. Remove the u...

Embodiment 2

[0079] [Example 2] Developmental toxicity verification of the developmental toxic exogenous compound azithromycin screened out based on Example 1

[0080] 1. Experimental animals

[0081] SPF grade healthy Kunming mice were purchased from Hubei Provincial Center for Disease Control and Prevention, animal license number: SCXK (Hubei), No.2017-0018. This study was approved by the Ethics Committee of Wuhan University Medical Center, and was carried out in strict accordance with the relevant treatment guidelines of international laboratory animal protection certification and evaluation institutions.

[0082] 2. Animal handling

[0083] Healthy and adult Kunming mice, weighing 25 ± 5 g for females and 30 ± 5 g for males, were purchased from Hubei Provincial Center for Disease Control and Prevention. At 6:00 every night, the male and female were caged at a ratio of 2:1, and the vaginal plug and sperm smear were checked the next morning to determine whether the female mice were suc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a cell model for screening developmental toxicity exogenous compounds by taking 11beta-HSD2 as a target, as well as a construction method and application of the cell model. Human WJ-MSCs is adopted as target cells, luciferase reporter genes containing 11beta-HSD2 promoters are transfected into the target cells to obtain the cell model, and the model has two indexes: 11beta-HSD2 gene expression and luciferase activity of a reporter system. By means of the cell model, 11beta-HSD2 gene expression and luciferase activity of a tested compound treatment group are reduced, andit is prompted that the tested compound has developmental toxicity. The cell model for screening developmental toxicity exogenous compounds has the characteristics of high specificity, high sensitivity, high stability, high feasibility and the like, can be used for high-throughput screening, and has important significance for rapid screening of developmental toxicity exogenous compounds.

Description

technical field [0001] The invention belongs to the field of drug toxicology, and relates to a cell model for screening developmental toxic exogenous compounds with 11β-HSD2 as a target, a construction method and application thereof. Background technique [0002] Developmental toxicity of exogenous compounds refers to the structural or functional damage caused by exogenous compounds in the offspring before sexual maturity, including structural deformities, dysfunction, growth retardation and even death. Developmental toxicity is one of the important contents of safety evaluation of exogenous compounds. Most of the current evaluation methods use the results of the overall animal reproduction experiment as a measure of the developmental toxicity of exogenous compounds, but there are disadvantages such as large amount of animals used, long experimental period, poor specificity, low sensitivity, and the inability to elucidate its mechanism from the cellular or molecular level. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/85C12N15/65C12Q1/02
CPCC12N5/0668C12N15/85C12N15/65G01N33/5014C12N2510/00C12N2800/107
Inventor 汪晖陈廖斌徐丹文印宪齐勇建李斌张棋
Owner WUHAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com