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Quality sensor for gene detection as well as preparation method and application of quality sensor

A gene detection and mass-based technology, applied in the field of mass-based sensors, can solve the problems of complex single-stranded DNA sample preparation, complex hybridization system, and inapplicability, and achieve fast chemical reaction speed, good scalability, and cost-saving effects

Active Publication Date: 2015-05-27
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the non-specific adsorption of biomacromolecules, it is easy to cause false detection.
In addition, the hybridization system is complicated, and the single-stranded DNA sample preparation of the sample to be tested is complicated, and it cannot be applied in actual detection.

Method used

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  • Quality sensor for gene detection as well as preparation method and application of quality sensor
  • Quality sensor for gene detection as well as preparation method and application of quality sensor
  • Quality sensor for gene detection as well as preparation method and application of quality sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] E.coliO157: Rapid detection of H7 (principle such as figure 1 As shown, the left is before EcoRV digestion, the right is after EcoRV digestion)

[0066] a) Close the silica surface of the non-sensitive position on the micro-cantilever sensor, and self-assemble a monolayer of polyethylene glycol silane (PEG-silane) on the silica surface to reduce the damage to the sensor due to non-specific adsorption Influence, the formula of polyethylene glycol silane solution is polyethylene glycol silane, absolute ethanol and deionized water, the volume ratio is 1:100:1, the reaction temperature is 75°C, and the soaking time is 90 minutes.

[0067] b) Self-assembled mercaptobiotin monolayer on the gold-coated surface at the sensitive position of the microcantilever sensor. Soak the micro-cantilever sensor in 1mM Sulfo-NHS-SS-Biotin PBS buffer for 8 hours, the NHS group in the Sulfo-NHS-SS-Biotin molecule is easily hydrolyzed to form mercaptobiotin and mercaptopropionic acid, Then a...

Embodiment 2

[0079] Rapid detection of hepatitis B virus:

[0080] a) Close the silica surface of the non-sensitive position on the micro-cantilever sensor, and self-assemble a layer of polyethylene glycol silane (PEG-silane) monolayer on the silica surface to reduce the impact on the micro-cantilever due to non-specific adsorption The influence caused by the sensor, the formula of polyethylene glycol silane solution is polyethylene glycol silane, absolute ethanol and deionized water, the volume ratio is 1:100:1, the reaction temperature is 75°C, and the soaking time is 90 minutes.

[0081] b) Self-assembled mercaptobiotin monolayer on the gold-coated surface at the sensitive position of the microcantilever sensor. Soak the microcantilever in 1mM Sulfo-NHS-SS-Biotin PBS buffer for 8 hours, the NHS group in the Sulfo-NHS-SS-Biotin molecule is easily hydrolyzed to form mercaptobiotin and mercaptopropionic acid, and then Formation of a mercaptobiotin monolayer on the gold surface of a microc...

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Abstract

The invention relates to the field of manufacturing of a sensor, particularly relates to a quality sensor for gene detection, and provides a quality sensor for gene detection. The quality sensor comprises a quality sensor body. The quality sensor is characterized in that a polyethylene glycol monolayer is arranged on the surface of a non-detected sensitive position of the quality sensor; a thiol biotin monolayer is arranged on the surface of the detected sensitive position of the quality sensor; a streptavidin layer is grafted on the thiol biotin monolayer. The quality sensor provided by the invention has the main advantages of real-time online detection, accurate result, fast detection speed, economic cost and good expansibility.

Description

technical field [0001] The invention relates to the field of sensor manufacturing, in particular to a quality sensor for gene detection. Background technique [0002] Gene detection technology is widely used in the detection of infectious diseases and genetic diseases. Gene sequencing technology that relies on DNA sequence specificity, DNA chip technology that relies on DNA hybridization, gene amplification technology that relies on PCR reactions, and gel electrophoresis technology that separates DNA fragments of different lengths are used alone or in combination for genetic testing. . Restriction endonucleases can specifically bind to specific sites within or near a DNA sequence known as a restriction enzyme recognition sequence, and cut double-stranded DNA. Restriction fragment length polymorphism analysis technology, that is, DNA fragments of different lengths produced by specific restriction endonuclease digestion of the DNA genome, separated by high-voltage pulse elec...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12M1/34
CPCC12Q1/6825C12Q2521/301C12Q2563/131C12Q2531/113
Inventor 徐铁刚李昕欣许鹏程于海涛
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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