The invention relates to a high-sensitivity gradient semi-quantitative immunochromatography detection test strip. The test strip comprises test paper and a sample cup; the test paper comprises a supporting layer, an adsorption layer and a protective layer; the adsorption layer sequentially comprises a sample pad, a conjugate pad, a
cellulose membrane layer and a water absorption material layer; a detection line T1, a detection line T2 and a
quality control line are arranged on the
cellulose membrane layer; the conjugate pad is made of
glass fiber cotton adsorbed with a nano material labeled
antigen; the sample cup is a container for diluting a sample, and a target
monoclonal antibody diluted by a gold-labeled
protein resuspension is pre-fixed in the sample cup. According to the test paper detection method, the two detection lines which do not interfere with each other can be prepared, and gradient semi-quantification is realized; the
antibody is independently dried in the sample cup, so that the
antibody is prevented from being folded and damaged in the labeling process, and the dosage of the antibody can be accurately controlled; the nano-particle labeled
artificial antigen is used as a probe, each antibody intercepted on the detection line T2 can be accurately combined with two labeled antigens, so that
signal self-amplification is realized; and compared with a traditional method, the method is higher in sensitivity.