Diluent capable of improving stability of acridinium ester antigen-antibody conjugate and reducing background and preparation method thereof

Abstract

The invention provides a diluent capable of improving the stability of an acridinium ester antigen-antibody conjugate and reducing the background. The diluent comprises the components of 5 to 7g of Tris-base, 3.5 to 4.0 mL of concentrated hydrochloric acid, 9 to 11 g of polyethylene glycol-6000, 0.95 mL to 1.05 mL of 10% lauryl sodium sulfate, 9.5 mL to 10.5 mL of Tween, 0.8 to 1.0 g of ethylene diamine tetraacetic acid disodium salt, 9 to 11 g of sodium caseinate, 0.95 mL to 1.05 mL of Proclin 300, 9.5 to 10.5 mL of triton X-100, and 1000mL of purified water for an in-vitro diagnostic reagent. The diluent has the advantages that the components are few; polyethylene glycol 6000, sodium dodecyl sulfate, tween and several active agents are mixed for use, meanwhile, a Tris-base buffer solution system is used, and multiple raw materials play a mutual synergistic effect, so that the diluent has a better protection effect on acridinium ester labeled antigens and antibodies, can be stored for a long time, can provide good blocking property and protectiveness for the antibodies, and reduces non-specific reactions; meanwhile, the material cost is saved, the specificity, stability, repeatability and other properties of the reagent are not influenced, and the reagent is suitable for popularization and application.

Description

technical field [0001] The invention relates to the technical field of immunoassay. [0002] Specifically, it relates to a diluent capable of improving the stability of an acridinium ester antigen-antibody conjugate and reducing background and a preparation method thereof. Background technique [0003] Acridine salts and related compounds have proven to be very advantageous chemiluminescent labels, with stability, activity and sensitivity exceeding those of radioactive isotopes. Acridine esters can react with any protein containing amino groups. Under alkaline conditions, NHS will leave, and acridinium esters will bind to proteins with a stable amide bond to form acridine compounds. After the reaction is complete, excess acridinium salts are removed through a desalting column. [0004] In the presence of alkaline hydrogen peroxide, acridine-labeled proteins can emit light without enzymatic catalysis. After adding the excitation reagent, the system releases photons immedia...

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Problems solved by technology

[0005] However, in the specific implementation process, the acridinium ester antigen-antibody conjugate is not as stable as imagined. It is easy to be hydrolyzed in the general buffer solution, and the reagent is prone to instability. During the long-term storage process, the background is usually easy to increase. , due to the poor effect of preserving the diluent, long-term storage, the protection is reduced, so the non-specific binding is increased, which will greatly reduce the performance and stability of the reagent

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