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Aftosa totivirus particle vaccine composition as well as preparation method and application thereof

A technology of vaccine composition and foot-and-mouth disease virus, which is applied in the field of foot-and-mouth disease whole virus particle vaccine composition and its preparation, vaccine composition and its preparation, can solve the problems of death, affecting meat animal food safety, animal side effects, etc., and achieve Efficacy of reducing risk of transmission, protective potency and efficacy

Active Publication Date: 2015-04-29
吕宏亮 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

FMD inactivated vaccine has good immunogenicity and plays an important role in the prevention and control of FMD, but some host cell residual proteins, nucleic acids, bovine serum albumin, chemical reagents, etc. If not controlled and removed, vaccinated animals can cause severe side effects or even death in animals, especially may cause cancer in animals or affect the food safety of meat animals

Method used

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  • Aftosa totivirus particle vaccine composition as well as preparation method and application thereof
  • Aftosa totivirus particle vaccine composition as well as preparation method and application thereof
  • Aftosa totivirus particle vaccine composition as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The preparation of embodiment 1 foot-and-mouth disease whole virus particle labeled antigen

[0033] Include the following steps:

[0034] a) BHK-21 cells are cultured in full suspension in a 100,000L bioreactor, and the cell density reaches 3-5×10 6Inoculate foot-and-mouth disease virus cell-adapted strain (porcine foot-and-mouth disease virus / Mya98-XJ-2010 strain, prepared by Inner Mongolia Biwei Antai Biotechnology Co., Ltd.) according to the multiplicity of infection MOI0.01-0.1 of the virus, and prepare the virus stock solution with a stirring speed not exceeding 40rpm, cultivated for 4 days to harvest the virus liquid, use a preparative low-speed continuous flow centrifuge to remove cell debris, and harvest the supernatant and sediment at the same time, and the sediment was lysed in the presence of 0.2% Triton-X-100. The infected cells and cell membrane fragments were lysed. After repeated freezing and thawing 3 times, ultrasonication 3 times (maximum output powe...

Embodiment 2

[0054] The preparation of embodiment 2 foot-and-mouth disease whole virus particle vaccine composition

[0055] 1, 13 micrograms of FMD whole virus particle labeled antigen, prepared in Example 1;

[0056] 2. Stable cryoprotectant: composed of the following substances by weight: 300 microliters of glycerol, polyoxypropylene glyceryl ether and polyethylene glycol 300 mixed in a volume ratio of 1:1:1, 200.02 mg of Tween and 50 mM 1 ml of phosphate buffer;

[0057] 3. Mix the FMD whole virus particle labeled antigen in the above 1 and 2 with a stable cryoprotectant, and adjust the pH of the vaccine to 8.0.

Embodiment 3

[0058] The preparation of embodiment 3 foot-and-mouth disease whole virus particle vaccine composition

[0059] 1, 20 micrograms of FMD whole virus particle labeled antigen, prepared in Example 1;

[0060] 2. Stable cryoprotectant: composed of the following substances by weight: 300 microliters of glycerol, 800.02 milligrams of Tween and 1 milliliter of a 1:1 mixture of 50 mM phosphate buffer and Tris buffer;

[0061] 3. Mix the FMD whole virus particle labeled antigen in the above 1 and 2 with a stable cryoprotectant, and adjust the pH of the vaccine to 7.5.

[0062] The preparation of embodiment 3 foot-and-mouth disease whole virus particle vaccine composition

[0063] 1.5 micrograms of FMD whole virus particle labeled antigen, prepared in Example 1;

[0064] 2. Stable cryoprotectant: composed of the following substances by weight: 400 microliters of a mixture of glycerol and polyoxypropylene glyceryl ether mixed according to a volume ratio of 1:1, 800.02 mg of Tween and 1...

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Abstract

The invention discloses an aftosa totivirus particle vaccine composition as well as a preparation method and an application thereof. The aftosa totivirus particle vaccine composition disclosed by the invention contains totivirus particles of aftosa virus and a stable cryoprotectant, wherein the stable cryoprotectant is composed of the following matters: polyhydric alcohol, a buffer solution and a nonionic surfactant. The stable cryoprotectant provided by the invention stabilizes the vaccine antigen, protects the titer and the effect of the marker vaccine, protects the antigen stability of the marker vaccine or the antigen stability of the marker antigen library in a production process and a cold chain process, and can maintain the titer or the activity of the marker vaccine for a longer time under liquid and refrigeration conditions.

Description

technical field [0001] The present invention relates to a vaccine composition and its preparation method and application, in particular to a foot-and-mouth disease whole virus particle vaccine composition and its preparation method and application. The present invention belongs to the field of medical biotechnology Background technique [0002] Foot-and-mouth disease (FMD) is a severe contact infectious disease of cloven-hoofed animals caused by foot-and-mouth disease virus (FMDV), which mainly harms cattle, sheep, pigs, camels, etc., and the incidence rate is extremely high. High, the propagation speed is extremely fast. The World Organization for Animal Health lists it as the first category A zoonotic disease. Although the mortality rate of the disease is not high (except for young animals), it can cause a decline in animal production performance, and culling animals requires a lot of manpower, material and financial resources, and it will also affect international trade,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/135A61K47/26A61K47/34A61P31/14A61K47/10
Inventor 张澍吕宏亮
Owner 吕宏亮
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