Micro-fluidic chip for detecting multiple infection markers in peripheral blood through multi-channel ELISA

Abstract

The invention relates to the technical field of micro-fluidic chip ELISA detection, in particular to a micro-fluidic chip for detecting multiple infection markers in peripheral blood through multi-channel ELISA. The micro-fluidic chip comprises a substrate, a sample adding pool, a capillary channel and a detection area. A serum sample is fed into the sample adding pool through a blood sampling port, driving is carried out through ultrasonic waves, infection marker antigen molecules in a serum sample enter the detection area through the capillary channel, and the infection marker antigen molecules in the serum sample are captured by infection marker antibodies of enzyme-linked immunosorbent assay in the reaction tank; full-automatic chip detection is realized by using an ELISA detection micro-fluidic chip technology, and human interference is not needed; and the detection repeatability of the same sample is high, and the detection result has high stability and reliability.

Description

technical field [0001] The invention relates to the technical field of microfluidic chip ELISA detection, in particular to a microfluidic chip for detecting multiple infection markers in peripheral blood by multi-channel ELISA. Background technique [0002] Sepsis is a life-threatening multiple organ dysfunction caused by an abnormal host response to infection. According to statistics, about 30 million people around the world suffer from sepsis every year, and the fatality rate is as high as 20%-50%. Early diagnosis of sepsis is of great significance for prompt use of antibiotics and fluid resuscitation. However, in actual clinical practice, most patients with sepsis have no clear evidence of infection in the early stage of the disease, so its early diagnosis is very difficult. [0003] The pathogenic mechanism of critically ill infected patients is complex and diverse with high heterogeneity. The mechanism of immune imbalance caused by activation of inflammatory factors a...

AI Technical Summary

Problems solved by technology

Among them, the detection range of colloidal gold method is limited and the accuracy is not high; the application range of fluorescence immunoassay is still limited at present, and most of them are limited to the fields of bacteria, virus and skin activity, and there are defects such as non-specific staining; these detection methods involve complex components, The reaction process has cumbersome steps, poor stability, and many influencing factors

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