246 results about "Gliadin" patented technology

The invention relates to an application of a Zea mays grain transcription factor in the aspect of regulation and control of alcohal-soluble proteins. The factor comprises a base sequence shown in SEQ ID NO: 1. The protein ZmbZIP22 encoded by the sequence can be directly bonded to a 27kDa gamma-gliadin promoter and activate the 27kDa gamma-gliadin promoter. Gene-deficient mutant plants are obtained through carrying out Zea mays immature-embryo conversion by taking a gene fragment, shown in SEQ ID NO: 2, of ZmbZIP22 as a guide RNA by using a CRISPR-Cas9 technology. Compared with wild type grains, transgenic mutants have Zea mays grains with irregular and relative-thin protein body shells, the content of alcohal-soluble proteins in mature grains is remarkably lowered, the content of essential amino acids such as lysine, which are deficient to the conventional Zea mays, in the mature grains is remarkably increased, and thus, the nutritional quality of Zea mays is improved.

The present invention discloses a preparation method of a stable high internal phase gelatinous wheat gliadin Pickering emulsion. The preparation method comprises the following steps: (1) wheat gliadin is dissolved into an alcohol solution; (2) an chitosan acetic acid solution is prepared; (3) the obtained solution in the step (1) is poured into the chitosan acetic acid solution, homogenization is conducted, and the homogenized solution is subjected to rotary evaporation; (4) a pH value of the obtained solution in the step (3) is adjusted to be 4.0-6.0; and (5) the obtained solution in the step (4) is mixed with oil and the mixture is subjected to shear emulsification to obtain the stable high internal phase gelatinous wheat gliadin emulsion. The obtained wheat gliadin Pickering emulsion by the method is high in stability and good in viscoelasticity, and can be used for embedding a large amount of oil. The stable high internal phase gelatinous wheat gliadin Pickering emulsion is low in requirement for devices, and simple and easy to operate in the method, and has relatively good prospect in the application aspects of novel nutrient delivery carriers and food structure modification base materials.

The invention discloses a preparation method of an edible protein stable Pickering emulsion. The method comprises the following steps: (1) adding chitosan into an acetic acid solution to be fully hydrated; (2) adding wheat gliadin into an ethanol solution to be fully dissolved; (3) adding the material obtained in the step (2) into the material obtained in the step (1), and carrying out shearing and homogenizing; (4) evaporating the material obtained in the step (3) until the mass concentration of wheat gliadin composite colloidal particles is 0.5-5%; (5) centrifuging the material obtained in the step (4), discarding precipitates, and taking supernatant liquid; and (6) adding corn oil into the material obtained in the step (5), and carrying out shearing and homogenizing to obtain a stable Pickering emulsion. The emulsion prepared by the preparation method does not contain any surfactant or organic solvent and can be used for conveying carrier loads and encapsulating biological active substances and especially fat-soluble biological active substances.

A corn product removal process for removing zein and / or oil from dry-milled corn. Oils and / or zein are extracted from corn using ethanol. Corn solids are separated from the ethanol, oil and zein mixture produced by extracting. Thereafter, the ethanol, oil and zein mixture is membrane filtered to restrain zein from the mixture and pass an oil and ethanol mixture. The zein and / or oil may be concentrated and purified.

The invention discloses a rancidity removed walnut kernel containing balsam pear. The walnut kernel is prepared from the following raw materials by weight: 40-50 parts of walnut, 18-22 parts of balsam pear, 10-13 parts of banana, 12-14 parts of dark plum powder, 12-13 parts of hawthorn, 3-4 parts of green bean cake, 8-9 parts of coconut milk, 1.5-2 parts of Chinese angelica, 1.8-2 parts of wild chrysanthemum, 0.5-1 part of water chestnut stems, emulsifier, sesbania gum and zein. The method uses prolamin to carry out the coating treatment on walnuts, so as to isolate outside moisture and oxygen, inhibit oxidative rancidity process of walnuts and prolong the shelf life of walnut kernel; the added traditional Chinese medicines such as Chinese angelica and wild chrysanthemum enhance the health care effects of walnut kernel, such as clearing heat, calming the liver, enriching blood and invigorating the circulation of blood.

The present invention is in the field of diagnosing celiac disease. More particularly, the present invention relates to a method to diagnose celiac disease based on the level of anti-gliadin and anti-tissue transglutaminase antibodies.

A food composition comprises gluten, a gliadin or glutenin and food stuff such as an additive for chewing gum, a batter for frying, a dough, a seafood paste and a livestock paste. A food quality such as taste is improved in the composition.

The invention relates to a resveratrol oral administration drug delivery system prepared through the nanometer technology. The drug delivery system is made of the resveratrol and bearer material of wheat gliadin. By utilizing the characteristic that the nanometer granule is highly dispersed and that of biological adhesion of the bearer material, the contact area and contact time of the resveratrol and absorption region of the intestinal canal is increased, which can promote absorption of the small intestine. In addition, the resveratrol oral administration nanometer drug delivery system is distributed over the suitable auxiliary-material skeletal material to improve the dissolve velocity and degree of the resveratrol.

The present invention relates to compositions for ameliorating the symptoms of celiac disease or gluten sensitive enteropathy comprising egg yolk antibodies against gluten, including gliadin, high molecular glutenin, low molecular glutenin and mixtures of the three peptides. The antibodies may be produced by immunizing laying hens with immunogenic preparations of gluten and harvesting the eggs and egg yolks.

The invention discloses an edible membrane of cross-linking cereal gliadin and its preparation method, wherein the film comprises cereal alcohol soluble protein 100 weight parts, plasticizing agent 20-100 weight parts, hydrophobic modifier 0.5-1 weight part, anti-oxidant 0.05-0.1 weight parts, antiseptic agent 0-0.1 weight part and cross linking agent 1-7 weight parts. The film is made through plastic processing method and high temperature cross bonding reaction.

The invention discloses a polysaccharide / alcohol soluble protein composite membrane and a preparation method of the polysaccharide / alcohol soluble protein composite membrane. According to the polysaccharide / alcohol soluble protein composite membrane and the preparation method of the polysaccharide / alcohol soluble protein composite membrane, polysaccharide (including curdlan or curdlan / konjac glucomannan mixed polysaccharide) and alcohol soluble protein (including wheat alcohol soluble protein or corn alcohol soluble protein) are taken as raw materials, a plasticizer and an emulgator are taken as auxiliary elements, tape casting and drying are conducted after blending modification is conducted according to a physical method, and then the polysaccharide / alcohol soluble protein composite membrane is obtained. The raw materials of the composite membrane are natural renewable resources and are abundant and easy to obtain. The polysaccharide / alcohol soluble protein composite membrane prepared with the method is flat and smooth, has excellent mechanical property and barrier property and is high in water vapor permeability, oxygen permeability and light transmittance, capable of being degraded naturally and rapidly and free of poison and pollution. The polysaccharide / alcohol soluble protein composite membrane can be used as a food packaging film or a sustained-release material for food or drugs.

The invention discloses a method for extraction of maize yellow pigment and alcohol soluble protein from maize protein powder. The method includes: crushing the maize protein powder, then conducting sieving by a 60-100 mesh sieve, adding ethanol with a material-liquid ratio of 1:(8-16), conducting ultrasonic treatment at 200-600W for 10-50min by an ultrasound assisted extraction technique; centrifuging the raw material and the extraction solvent, then collecting the supernatant and performing reduced pressure distillation till an orange red oily liquid, and conducting drying to obtain a pigment product; drying the centrifuged precipitate, adding ethanol diluted to 60%-90% according to a material-liquid ratio of 1:(6-14), conducting ultrasonic treatment under ultrasonic power of 200-400W for 10-50min, performing centrifugation and taking the supernatant, adjusting isoelectric points, collecting alcohol soluble protein from the precipitate, and conducting drying to obtain the finished product. According to the invention, maize yellow pigment and alcohol soluble protein with high purity can be extracted in steps by one production process, the solvent can be recycled, the production cost is saved, the extraction efficiency is improved, and the operation is simple, so that the method is easy to realize industrialization.

The present invention provides an immunological detection method that can detect milk allergens, allergens of albumen, flour, buckwheat and peanut with high sensitivity in foods containing these allergens regardless they are denatured / native, and a detection kit to be used therefor. It is a method for detecting allergens by using 2 or more monoclonal antibodies recognizing native and denatured milk allergens, native and denatured albumen allergens, native and denatured flour allergens, native and denatured buckwheat allergens, and native and denatured peanut allergens, using asl casein which is the main protein of milk casein, β-lactoglobulin which is the main protein of whey, ovalubumin and ovomucoid which are main proteins of albumen, gliadin which is the main protein of flour, protein with a molecular weight of 24 kDa and 76 kDa which are main proteins of buckwheat, and Ara h1 which is the main protein of peanut as an index.

A hybrid vegetable protein is described, comprising a guest protein having the structure of prolamine and glutelin, and a host protein having the structure of globulin and albumin, obtained from vegetable grains, such as corn and soybean, respectively. Likewise, a method for obtaining said hybrid vegetable protein is described, which comprises the steps of extracting the guest and host proteins, carrying out an acidification thereof, and further applying a magnetic field to provoke their attachment, and finally adding an alkali to the attached proteins to obtain a hybrid vegetable protein at its isoelectric point. The protein thus produced has a value higher than 0.97 according to the PDCAAS rating.

A method of using resin chromatographic analysis antigenic to purify Phosphatidyl Choline belongs to the phospholipid purification technology. The invention takes Gliadin phospholipid as raw material to prepare ethanol solution with concentration of Gliadin phospholipid 3-5mg / mL, which is add into the D113-IIIFC resin chromatographic column at the flow rate of 1mL / min, and then elutes with 95% ethanol at the rate of 0.5-1mL / min, collects the elutes, evaporates and concentrates to remove solvents, uses vacuum drying to get the pale yellow and plastic-like products of PC. In this products, the content of PC is 94.02% (wt %), and the product yield is 80.74%.

Relating to the food industrial field, the invention discloses a method of continuously extracting corn oil, alcohol soluble protein and yellow pigment with corn gluten meal. The method includes: corn gluten meal crushing, petroleum ether extraction, filtration, concentration, salt washing, centrifugation, ethanol extraction, stirring, filtering and drying. Corn oil, alcohol soluble protein and yellow pigment are extracted continuously by a simple process. The method is simple and is convenient to operate, significantly improves the added value of corn gluten meal, compared with single product preparation, the method provided by the invention has the advantages of low production cost and easiness in industrial production, and is conducive to comprehensive development and utilization of corn gluten meal and improvement of economic benefits.

A 3D scaffold is prepared from the vegetative gliadin, the pore-forming agent chosen from edible salt, ammonium dicarbonate, ammonium acetate, etc. the polylactic acid or polyhydroxyacetic acid, and the biomedical material (collagen,chitosan, etc) through proportional mixing and natural shaping or die pressing. Its advantages are high degradability and bacterial resistance, low immunogenicity and proper mechanical strength.

A method for the development of biodegradable or edible film from wheat gluten protein has been revealed. For this purpose, a fraction of gliadin protein, on the basis of solubility, is recovered from ethanolic extract of wheat gluten protein to fabricate homogenous, transparent, heat sealable and water soluble edible films with novel functional and mechanical properties. To reduce film brittleness, glycerol was added in the formulation as a plasticizer. A three dimensional network of gliadin protein's fraction, water and plasticizer is formed by virtue of new hydrogen bonds, hydrophobic interactions and disulphide bonds when such films are produced by casting technique followed by drying. This network provides resistance to moisture, lipid and gas permeation together with glossy sheen when coated on a variety of substrates.

Gluten-free baked products, particularly gluten-free bread products, which exhibit properties comparable those made with wheat flour. Compositions and methods for preparation of gluten-free baked products. A conditioned protein or protein composite which functions to replace gluten in gluten-free flour. The conditioned protein comprises one more non-wheat cereal storage proteins, particularly prolamins, optionally, but preferably in combination with one or more co-proteins which function to facilitate formation and stabilize formation of a protein network, e.g., β-sheet network, that facilitates retention of CO2 for leavening. More specifically, protein composites of zein and or other non-wheat prolamins with co-proteins including casein, elastin or mixtures thereof conditioned at temperatures above the glass transition temperature of the protein mixture provide improved ingredients for preparation of gluten-free breads and other baked products. Sorghum or maize mutant flours with prolamins available for viscoelastic protein formation may also be used.

Decolorized and / or deodorized zein from corn products may be recovered in high yields using zeolite adsorbents. A solution of a zein-containing corn product in an aqueous alcohol solvent is contacted with a zeolite adsorbent under conditions effective for adsorption of color and odor impurities in the corn product onto the zeolite. Following this contact, the treated solution may be separated from the adsorbent and recovered, yielding substantially pure zein dissolved in the aqueous alcohol solvent. Optionally, the zein may be further purified by subsequently contacting the treated solution with an activated carbon adsorbent or a mixture of activated carbon and zeolite adsorbents to adsorb any residual color and / or odor impurities therefrom.

The invention provides a method for preparing a plant essential oil powder microcapsule. The method comprises the steps of preparing plant essential oil, embedding a wall material for the first time,drying by spray and embedding the wall material for the second time. According to the preparation method, plant essential oil is embedded in micropores through beta-cyclodextrin embedding, and membrane attached embedding is carried out with zein or wheat gliadin, a completely closed structure can be formed, and the essential oil has an embedding rate of 80-88 percent; and the stability of plant essential oil at normal temperature and high temperature can be improved by twice embedding, the volatility can be reduced, and the high temperature resistance can be reinforced.

A phytogenous gliadin microsphere solid as the medicine carrier is prepared by phase separation method. It can be mixed with the medicine in the mass ratio of (1-199):1.

The invention discloses a decoloration method of yellow zein, and belongs to the technical field of deep processing of agricultural and sideline products. According to the decoloration method, an attapulgite clay and kieselguhr clay complex is adopted as a compound decolorising agent for vibrating adsorption decoloration, the compound decolorising agent is centrifugally separated out after adsorption decoloration is completed, and a zein extraction solution is obtained; and the pH value of the zein extraction solution is adjusted to the protein isoelectric point, precipitating is carried out, zein wet powder is obtained, vacuum freeze drying is carried out, and a white zein product is obtained. All the performance indexes of the product can reach the food and drug grade safety standard, the zein is widely applied to the food and medicine industry, the additional value of corn gluten meal is remarkably improved, and comprehensive development and utilization of the corn gluten meal are promoted. In addition, the decoloration method has the beneficial effects of being simple, efficient, environment-friendly, low in cost and the like, and is suitable for large-scale production.

The biodegradable chewing gum with functions of nursing face, beautifying and slimming has the nursing face and beautifying components including serine, proline, lysine and sorbitol; and the slimming components including haw lipase, gamma-linolenic acid, xylitol, etc. It has biodegradable natural gum prepared with corn starch. The production process of the natural gum includes the steps of: mixing alcohol solution with corn starch, microwave heating to obtain alcohol corn protein, purification, and modification with chitosan and cross-linking agent. The chewing gum contains also proteoglycan, calcium lactate, sweetener, essence, pigment and glycerin.

Compositions, food products or beverages for ameliorating the symptoms of celiac disease or gluten sensitive enteropathy comprising egg yolk antibodies against gluten, including gliadin, high molecular glutenin, low molecular glutenin and mixtures of the peptides. The antibodies may be produced by immunizing egg laying fowl with immunogenic preparations of gluten and harvesting the eggs and egg yolks.

The invention provides a method for comprehensively preparing wheat polypeptide, wheat gliadin and wheat bran massage particles and application of the wheat polypeptide, the wheat gliadin and the wheat bran massage particles. By the method, the wheat polypeptide, the wheat gliadin and the wheat bran massage particles can be synchronously prepared from wheat bran, so that the utilization rate of the wheat bran is greatly improved; moreover, the using amount of an organic solvent is small in the whole preparation process, preparation conditions are simple, the process is easy to control, the environmental pollution is avoided, the demand of energy conservation and environmental protection is met, and the application value of the wheat bran is greatly improved. The wheat polypeptide and the wheat gliadin which are prepared by the method are applied to the preparation of skin care products and hair care products, and can achieve excellent effects; and the wheat bran massage particles used as massage particles are applicable to fancy soaps and bath creams, and can achieve excellent effects.

The invention discloses a method for preparing a novel compound protein fiber through enzyme treatment combined with electrostatic spinning. The method comprises the following steps: preparing a zein solution, preparing a casein solution, preparing wheat gluten suspension liquid, preparing a mixed solution of zein, casein and wheat gluten, processing the mixed solution of zein, casein and wheat gluten by glutamine transaminase in a cross linking way, performing the operation of electrostatic spinning for the mixed solution processed by the glutamine transaminase in a cross linking way, and obtaining the novel compound protein fiber. The method for preparing the novel compound protein fiber through enzyme treatment combined with the electrostatic spinning adopts the glutamine transaminase to catalyze the cross linking reaction of the zein, the casein and the wheat gluten, the novel compound fiber is prepared by the electrostatic spinning, the average diameter of the prepared fiber reaches the nanometer stage, and the breakthrough method is provided to improve regenerated fiber materials, so that natural protein fibers can be better applied.

The invention discloses a haynaldia villosa 1VS chromosome-specific molecular marker primer and an application thereof. By the aid of the molecular marker primer, haynaldia villosa 1VS chromosomes ina wheat background can be accurately and rapidly identified, and haynaldia villosa prolamin genes can be separated. The technical scheme includes that the specific molecular marker primer comprises 1VS-F2 / 1VS-R3 and 1VS-F8 / 1VS-R3, the 1VS-F2 molecular marker primer has a nucleotide sequence as shown in a sequence table SEQ ID NO.1, the 1VS-R3 has a nucleotide sequence as shown in a sequence tableSEQ ID NO.2, and the 1VS-F8 has a nucleotide sequence as shown in a sequence table SEQ ID NO.3. The haynaldia villosa 1VS chromosome-specific molecular marker primers 1VS-F2 / 1VS-R3 and 1VS-F8 / 1VS-R3 are applied to haynaldia villosa 1VS chromosome detection and cloning and separation of the prolamin genes on the haynaldia villosa 1VS chromosomes.

The invention discloses a preparation method for zein. The preparation method comprises the following steps: with corn gluten meal as a raw material, adding ethanol water to extract the corn gluten meal; carrying out filtering and separating so as to obtain zein extract; adding the zein extract into a resin for chromatography; after removal of impurities, eluting protein with salt-containing ethanol water; and successively carrying out concentration, ethanol recovery, washing and drying so as to obtain a finished zein product. The method has the advantages of low cost and small influence on the environment; and the obtained finished zein product has a light color, low impurity content and high purity and is suitable for industrial production.