The invention provides a method for comprehensively preparing wheat polypeptide, wheat gliadin and wheat bran massage particles and application of the wheat polypeptide, the wheat gliadin and the wheat bran massage particles. By the method, the wheat polypeptide, the wheat gliadin and the wheat bran massage particles can be synchronously prepared from wheat bran, so that the utilization rate of the wheat bran is greatly improved; moreover, the using amount of an organic solvent is small in the whole preparation process, preparation conditions are simple, the process is easy to control, the environmental pollution is avoided, the demand of energy conservation and environmental protection is met, and the application value of the wheat bran is greatly improved. The wheat polypeptide and the wheat gliadin which are prepared by the method are applied to the preparation of skin care products and hair care products, and can achieve excellent effects; and the wheat bran massage particles used as massage particles are applicable to fancy soaps and bath creams, and can achieve excellent effects.