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Preparation method of plant origion alcohrl soluble protein three dimentional support

A plant-derived, gliadin technology, applied in medical science, prosthesis, surgery, etc., can solve the problems of long preparation cycle and low porosity, and achieve low immunogenicity, high mechanical strength, and easy-to-obtain raw materials Effect

Inactive Publication Date: 2004-12-22
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above method can obtain scaffold materials with high compressive strength of tens of MPa, the porosity is low and the preparation cycle is long

Method used

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  • Preparation method of plant origion alcohrl soluble protein three dimentional support
  • Preparation method of plant origion alcohrl soluble protein three dimentional support
  • Preparation method of plant origion alcohrl soluble protein three dimentional support

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Mix zein alone or with a pore-forming agent (salt) at a ratio of 1:1 (weight ratio), sieve the salt through sieves of different meshes before mixing, and select a sieve of 70-100 meshes to obtain a pore-forming agent (salt) with a diameter of 76-150 microns. Table salt granules. Then put the mixture into a 10ml small beaker (2g / beaker), place it in an 80°C wet box to incubate after ultraviolet sterilization, and cover the mouth of the beaker with absorbent paper to prevent water droplets from directly entering the beaker. Take it out on the 5th day, soak it in 40°C water for 48 hours, then boil it in water at 60°C for 1 hour, and change 2-3 times of deionized water preheated to the predetermined temperature during the boiling process to ensure that the salt is completely dissolved. Then lyophilized and vacuum-dried, the measured compressive modulus was 11 MPa, and the porosity was 68.6%. Scanning electron microscope ultrastructural observation shows that the pore size ...

Embodiment 2

[0020] After mixing zein and pore-forming agent according to the above method, put 1 gram of protein into a cylindrical mold according to the amount of each sample, and take it out after compression molding with a pressure of about 800N. Each sample is placed separately in a container 60 ℃ deionized water in a small beaker to prevent the samples from sticking to each other in the water, and then directly boil it in a constant temperature water bath for 1 hour, and change the preheated deionized water 2-3 times during the boiling process to ensure The salt is completely dissolved. Then lyophilized and vacuum-dried, the measured compressive modulus was 10MPa. Scanning electron microscope ultrastructural observation shows that the pore size distribution is about 300 microns ( image 3 ).

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Abstract

A 3D scaffold is prepared from the vegetative gliadin, the pore-forming agent chosen from edible salt, ammonium dicarbonate, ammonium acetate, etc. the polylactic acid or polyhydroxyacetic acid, and the biomedical material (collagen,chitosan, etc) through proportional mixing and natural shaping or die pressing. Its advantages are high degradability and bacterial resistance, low immunogenicity and proper mechanical strength.

Description

technical field [0001] The invention relates to a method for preparing plant-derived protein, in particular to a method for preparing a three-dimensional scaffold of plant-derived prolamin. Belongs to the field of biotechnology. Background technique [0002] With the development of modern medicine, especially the development and popularization of human tissue and organ repair or transplantation technology, the demand for biomedical materials has become a major market. The requirements for biomedical materials, in addition to having good biocompatibility, non-toxicity, and biodegradability in vivo, must also have certain mechanical strength. In particular, the materials used for tissue engineering scaffolds also require a microporous structure with a certain strength, so that cells can adhere to the surface and inside; it is beneficial to the growth and reproduction of cells; it is beneficial to the exchange of nutrients and metabolic waste required by cells. exclusion. Or...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L31/04
Inventor 孙庆申王瑾晔
Owner SHANGHAI JIAO TONG UNIV
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