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Bionic nerve repair material with double-layer structure and preparation method of bionic nerve repair material

A nerve repair, double-layer structure technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of poor biocompatibility and poor radial compression performance of nerve repair materials

Inactive Publication Date: 2019-02-22
SHANGXIAN MINIMAL INVASIVE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome the disadvantages of poor suture performance and radial compression performance of nerve repair materials prepared by natural biological materials, and poor biocompatibility of nerve repair materials prepared by synthetic materials, the present invention proposes a method of coating after weaving, which can make the prepared nerve repair The material is easy to suture with the nerve stump, and has good flexibility and elasticity; the present invention provides a bionic nerve repair material with a double-layer structure and a preparation method thereof by comprehensively analyzing the requirements for the basic performance of the material for peripheral nerve repair. Skin-core double-layer structure, the core layer is a multi-channel structure that facilitates cell adhesion and directional growth, and the cortex improves the suture performance and radial compression performance of the overall repair material

Method used

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  • Bionic nerve repair material with double-layer structure and preparation method of bionic nerve repair material
  • Bionic nerve repair material with double-layer structure and preparation method of bionic nerve repair material
  • Bionic nerve repair material with double-layer structure and preparation method of bionic nerve repair material

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preparation example Construction

[0035] The preparation method of the bionic nerve repair material core layer among the present invention is:

[0036]1) Using chitosan and collagen as raw materials, prepare chitosan solution by dissolving, or prepare collagen gel by swelling, or prepare chitosan-collagen gel by blending chitosan solution and collagen gel The dissolving and swelling methods are as follows: placing chitosan and collagen in 0.1-1% (v / v) acetic acid aqueous solution.

[0037] 2) injecting chitosan solution, collagen gel or chitosan-collagen gel into the mold, immersing in liquid nitrogen to freeze, then freeze-drying and cross-linking to prepare the core layer of the material of the present invention. The schematic diagram of the mold is as image 3 As shown, the mold is made of stainless steel and has a cylindrical structure with multiple channels in the middle, which can simultaneously prepare multiple samples.

[0038] 3) PLGA, PGA fibers are prepared by weaving and recoating hollow conduits...

Embodiment 1

[0041] Example 1: A biomimetic nerve repair material with a double-layer structure

[0042] The preparation method is:

[0043] 1) dissolving chitosan in the acetic acid solution with concentration of 0.5% (v / v) to prepare a chitosan solution with a concentration of 2% (m / v), and swelling the collagen at a concentration of 0.5% (v / v) A collagen gel with a concentration of 1% (m / v) was prepared in an acetic acid solution, and the chitosan solution and the collagen gel were blended at a mass ratio of 1:2 to obtain a chitosan-collagen gel.

[0044] 2) Inject the chitosan-collagen gel into the mold in a volume of 1×10 -5 Immerse in liquid nitrogen at a speed of m / s to freeze, transfer to -80° refrigerator for 30 minutes, freeze-dry at -60° for 24 hours, and cross-link at room temperature in a 1% (m / v) genipin solution for 24 hours to obtain nerve cells. Core layer of repair material.

[0045] 3) Braid the PLGA multifilament with a molecular weight of 400,000 with a core, then c...

Embodiment 2

[0048] Example 2: A biomimetic nerve repair material with a double-layer structure

[0049] The preparation method is:

[0050] 1) dissolving chitosan in the acetic acid solution with concentration of 0.8% (v / v) to prepare the chitosan solution with concentration of 2.5% (m / v), and swelling the collagen at a concentration of 0.5% (v / v) A collagen gel with a concentration of 0.5% (m / v) was prepared in an acetic acid solution, and the chitosan solution and the collagen gel were blended at a mass ratio of 1:4 to obtain a chitosan-collagen gel.

[0051] 2) Inject the chitosan-collagen gel into the mold in a volume of 5×10 -5 The speed of m / s was immersed in liquid nitrogen to freeze, transferred to -80 ° refrigerator for 60 min, freeze-dried at -60 ° for 24 h, and cross-linked at room temperature in a 1.5% (m / v) genipin solution for 48 h to obtain nerve cells. Core layer of repair material.

[0052] 3) Dissolving PLGA with a molecular weight of 300,000 in chloroform to prepare ...

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Abstract

The invention discloses a bionic nerve repair material with a double-layer structure. The bionic nerve repair material is formed by compositing a skin layer and a core layer; the core layer is of a multichannel columnar structure and is prepared from a chitosan solution and collagen gel or chitosan-collagen gel with a gradient condensation method; the skin layer is prepared from high polymer fiberthrough cored weaving and coating; or the skin layer is prepared from the high polymer fiber through electrostatic spinning; or the skin layer is prepared from the chitosan solution and collagen gelor chitosan-collagen gel with an extrusion method. The core layer of the nerve repair material has good cell compatibility, the multichannel structure is more beneficial to growth of nerve cells, thesuture performance and radial compression performance of the material can be improved by the aid of the skin layer, and clinical operation is facilitated.

Description

technical field [0001] The invention belongs to the field of peripheral nerve repair in biomedical engineering, and specifically relates to a bionic nerve repair material with a double-layer structure and a preparation method thereof, specifically, the suture performance and radial compression of the overall material are improved by compounding the cortex and the core layer Performance, both the skin layer and the core layer are degradable materials, and the composite material has good suture performance, radial compression performance and good cell compatibility. Background technique [0002] Peripheral nerve injuries occur in a large number of people every year, and its treatment has always been an important challenge in surgery. Autologous nerve transplantation is still the gold standard for the treatment of peripheral nerve injuries. However, the available autologous nerves are very limited and not easy to obtain. While autologous nerve transplantation brings benefits, i...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/24A61L27/20A61L27/18A61L27/50
CPCA61L27/18A61L27/20A61L27/24A61L27/50A61L2430/32C08L5/08C08L67/04
Inventor 李瑞欣蔡盼盼李洪谊
Owner SHANGXIAN MINIMAL INVASIVE INC
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