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Preparation method of plant origion alcohrl soluble protein three dimentional support

A plant-derived, gliadin technology, which is applied in medical science, prosthesis, surgery, etc., can solve the problems of long preparation cycle and low porosity

Inactive Publication Date: 2006-05-24
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the above method can obtain scaffold materials with high compressive strength of tens of MPa, the porosity is low and the preparation cycle is long

Method used

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  • Preparation method of plant origion alcohrl soluble protein three dimentional support
  • Preparation method of plant origion alcohrl soluble protein three dimentional support
  • Preparation method of plant origion alcohrl soluble protein three dimentional support

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Embodiment 1

[0018] After mixing the zein and the pore-forming agent according to the above method, put it into a cylindrical mold according to the amount of 1 gram of protein for each sample, compress it with a pressure of about 800N, and then take it out. 60C deionized water in a small beaker to prevent the samples from sticking to each other in the water, and then directly boil in a constant temperature water bath for 1 hour, and change the preheated deionized water 2-3 times during the boiling process to ensure the salt completely dissolved. It was then freeze-dried under vacuum, and the compressive modulus was determined to be 10 MPa. Scanning electron microscope ultrastructural observation shows that the pore size distribution is about 300 microns ( image 3 ).

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Abstract

A method for preparing a plant-derived gliadin three-dimensional scaffold. Belongs to the field of biotechnology. The plant-derived prolamin is used as the main component, and pore-forming agents of different particle sizes are mixed according to different mass ratios, protein: pore-forming agent = 1:0-1:10, or protein is used alone, and pressed into a predetermined shape by using a mold. shape. The invention can obtain a degradable three-dimensional porous bracket with high mechanical strength, adjustable pore diameter and porosity after thermoforming treatment. In the in vitro cell culture experiment, the cell morphology observation, cell viability, and adhesion test all proved that the protein has good cytocompatibility and possesses the material characteristics required by tissue engineering, thus finding a new method for tissue engineering. A new type of biomedical material with a wide range of sources, degradable, low immunogenicity, antibacterial properties, and appropriate mechanical strength.

Description

technical field [0001] The present invention relates to a preparation method of plant-derived protein, in particular to a preparation method of plant-derived prolamin three-dimensional scaffold. It belongs to the field of biotechnology. Background technique [0002] With the development of modern medicine, especially the development and popularization of the repair or transplantation technology of human tissues and organs, the demand for biomedical materials has become a big market. The requirements for biomedical materials are not only good biocompatibility, non-toxicity, and in vivo degradability, but also certain mechanical strength. In particular, the materials used for tissue engineering scaffolds also require a certain strength in the form of a microporous structure, so that cells can adhere to the surface and inside; it is conducive to the growth and reproduction of cells; it is conducive to the exchange of nutrients required by cells and metabolic waste. exclusion....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L31/04
Inventor 孙庆申王瑾晔
Owner SHANGHAI JIAOTONG UNIV
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