Method of separating and purifying phosphatidyl choline from phospholipid by resin chromatography method

Abstract

A method of using resin chromatographic analysis antigenic to purify Phosphatidyl Choline belongs to the phospholipid purification technology. The invention takes Gliadin phospholipid as raw material to prepare ethanol solution with concentration of Gliadin phospholipid 3-5mg / mL, which is add into the D113-IIIFC resin chromatographic column at the flow rate of 1mL / min, and then elutes with 95% ethanol at the rate of 0.5-1mL / min, collects the elutes, evaporates and concentrates to remove solvents, uses vacuum drying to get the pale yellow and plastic-like products of PC. In this products, the content of PC is 94.02% (wt %), and the product yield is 80.74%.

Description

technical field [0001] The invention discloses a method for separating and purifying phosphatidylcholine from phospholipids by resin chromatography, belonging to the technical field of phospholipid separation and purification. Background technique [0002] Soy lecithin is a mixture of various phospholipids, and its main components are phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS) and other substances. Wherein phosphatidylcholine (phosphatidylcholine, PC), commonly known as lecithin, is a kind of natural amphoteric surfactant widely distributed in animals and plants, its chemical structural formula is as follows, it is nontoxic, non-irritating, easy biodegradation, Has special physiological effects. Different phospholipids in soybean phospholipids have structural similarities. PC is more polar than other phospholipids. When pH=7, the N of PC molecule choline has a positive charge, and the O of phosphoric acid has ...

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Problems solved by technology

[0005] Phosphatidylcholine has important medicinal value, and the separation of phosphatidylcholine from phospholipids has always been an important subject of phospholipid research at home and abroad. At present, the separation methods studied by domestic and foreign scholars mainly include low-carbon alcohol method, ultrafiltration method, and precipitation method. , chromatography and supercritical fluid method, etc., among them, chromatography can be divided into the following types according to different fillers: aluminum oxide column chromatography, silica gel column chromatography, ion exchange column chromatography, the above methods are mainly It is aimed at the separation of two phospholipids, PC and PE, and the purity of the obtained PC product is about 90%. The aluminum oxide column has a strong adsorption capacity for other phospholipids and can only elute the more polar PC from the column. Down, the elution period is short but lysophospholipids will be produced in the process; the silica gel column can realize the separation of PC, PE, PI and other phospholipids, but the elution period is longer; the research on ion exchange resin separation of phospholipids is less, only The feasibility of resin separation of phospholipids has been studied, but the research on specific process parameters is not systematic enough

According to the structural characteristics of the isolate, a method for separating phosphatidylcholine by ion-exchange resin is proposed, and the ion-exchange resin can be regenerated and reused, has low cost, and is more suitable for large-scale industrial preparation. At present, there are no reports on this aspect at home and abroad. article

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