LAMP (Loop-Mediated Isothermal Amplification) primer group, kit and detection method for detecting lotus rot pathogen

Abstract

The invention relates to technical field of biology and in particular to discloses a LAMP (Loop-Mediated Isothermal Amplification) primer group, a kit and a detection method for detecting lotus rot pathogen. A group of primer group F3 / B3 and FIP / BIP is designed by aiming at a lotus rot pathogen translation elongation factor (TEF-1 alpha) gene sequence, the primer group or the kit containing the primer group is used for detecting the lotus rot pathogen by virtue of LAMP. The LAMP primer group and the kit have the advantages of low cost, easiness in operation, simplicity, rapidness, visual detection result, judgment capability based on naked eyes, high sensitivity, strong specificity and the like, are applicable to lotus (lotus root) quarantine for introducing seeds, seedling and breeding lotus root quality test, lotus rot pathogen field early diagnosis and the like in basic departments, and have wide and practical application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a LAMP primer set, a kit and a detection method for rapidly detecting lotus spoilage pathogens. Background technique [0002] The lotus is an important economic plant, and its underground stems - lotus root and lotus seeds have always been vegetables and tonics that Chinese people often eat. With the prosperity of China's market economy, lotus has become a competitive product in some areas and has become a pillar industry for the development of local economy and culture. In recent years, in wetlands such as Sanshui Lotus World, Panyu Lianhua Mountain, Dongguan Qiaotou and other wetlands where lotus cultivation is concentrated in the Pearl River Delta, rot has occurred on a large scale, affecting normal production and tourism industries. The rot disease caused by Fusarium commune is one of the most common and serious diseases in the lotus planting area. It can occur durin...

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Problems solved by technology

[0003] In recent years, it has been reported that PCR-based methods have been successfully used in the detection of lotus (lotus) spoilage bacteria (methods, kits and primers for detecting lotus fungal spoilage pathogens carried by lotus seeds, patent number: 201310715070.0), and the PCR method has been used in The operating time and the specificity and sensitivity of the detection are greatly improved compared with the conventional method. However, the PCR method must have an expensive and sophisticated temperature cycle device, and its detection time is still relatively long (3 to 6 hours). Only with the help of an electrophoresis instrument can the positive or negative of the amplification product be distinguished, which is costly and difficult to operate, and the reaction process is easily affected by pollutants, which makes this method unable to meet the needs of actual detection

At present, there is no relevant technical report on the application of LAMP technology for the detection of lotus rot

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