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Colorimetric sensor for enhancing MOFs (Metal-Organic Frameworks) enzyme-like activity based on aptamer as well as preparation method and application of colorimetric sensor

A colorimetric sensor and aptamer technology, applied in the direction of chemical reaction of materials, material analysis by observing the impact on chemical indicators, etc., can solve the problems of high operating cost, strong toxicity, and expensive equipment. , to achieve high accuracy and good stability

Active Publication Date: 2021-11-09
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The wide application of pesticides plays a positive role in promoting agricultural production. However, pesticides have disadvantages such as refractory degradation and strong toxicity, and are easily enriched in water, soil, fruits and vegetables. Therefore, it is necessary to detect pesticide residues
Traditional detection methods for pesticide residues include high-performance liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS / MS), etc. These methods are difficult to achieve rapid on-site detection due to expensive equipment and high operating costs
[0003] At present, in order to realize the rapid detection of pesticide residues, rapid detection methods such as fluorescence, surface-enhanced Raman scattering and electrochemistry have been constructed based on the specific recognition of nucleic acid aptamers and pesticides, but the above methods rely on specific instruments and equipment to output corresponding Optical and electrical signals, which largely limit its portable applications

Method used

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  • Colorimetric sensor for enhancing MOFs (Metal-Organic Frameworks) enzyme-like activity based on aptamer as well as preparation method and application of colorimetric sensor
  • Colorimetric sensor for enhancing MOFs (Metal-Organic Frameworks) enzyme-like activity based on aptamer as well as preparation method and application of colorimetric sensor
  • Colorimetric sensor for enhancing MOFs (Metal-Organic Frameworks) enzyme-like activity based on aptamer as well as preparation method and application of colorimetric sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Preparation and functionalization of MOFs:

[0041] Weigh 5 mM Zn(NO 3 ) 2 ·6H 2 O was dispersed in 50 mL of methanol solution, and 10 mM dimethylimidazole was weighed and dispersed in 50 mL of methanol solution; under the condition of magnetic stirring, the above two solutions were mixed, incubated at room temperature for 24 h, and then heated at 10000 r Centrifuge at a speed of 1 / min; wash with methanol three times, and dry in vacuum at 60°C for 12 h to obtain ZIF-8. Functionalized ZIF-8 was prepared by incubating 10 μL of aptamer solution with a concentration of 100 nM and 60 μL of 0.83 mg / mL ZIF-8 for 10 min, and the aptamer solution was ACGCGAATCGGAGTTGGGGGT.

[0042] figure 1 It is the SEM picture of ZIF-8. It can be seen from the figure that the ZIF-8 nanocrystal background has no impurities, indicating that the prepared ZIF-8 has a relatively stable structure and uniform size.

[0043] figure 2 is a TEM image of ZIF-8. It can be seen from the figure ...

Embodiment 2

[0049] (1) Preparation and functionalization of MOFs:

[0050] Weigh 4 mM Zn(NO 3 ) 2 ·6H 2 O was dispersed in 45 mL of methanol solution, and 9 mM dimethylimidazole was weighed and dispersed in 45 mL of methanol solution; under the condition of magnetic stirring, the above two solutions were mixed, incubated at room temperature for 24 h, and then heated at 9000 r Centrifuge at a speed of 1 / min, wash with methanol three times, and dry in vacuum at 40°C for 24 h to obtain ZIF-8. Mix 15 μL of 150 nM aptamer solution and 50 μL of 1.33 mg·mL -1 ZIF-8 was incubated for 15 min to prepare functionalized ZIF-8, and the aptamer solution was ACGCGAATCGGAGTTGGGGGT.

[0051] (2) Construction of a colorimetric sensor based on aptamer-enhanced enzymatic activity of MOFs:

[0052] Prepare a series of standard solutions of fipronil, the concentrations are 0.1 μM, 0.2 μM, 0.4 μM, 0.5 μM, 0.8 μM, 1 μM, 2 μM, 3 μM, 4 μM, 5 μM. 15 μL of different concentrations of fipronil standard solutio...

Embodiment 3

[0060] (1) Preparation and functionalization of MOFs:

[0061] Weigh 6 mM Zn(NO 3 ) 2 ·6H 2 O was dispersed in 55 mL of methanol solution, and 11 mM dimethylimidazole was weighed and dispersed in 55 mL of methanol solution; under the condition of magnetic stirring, the above two solutions were mixed, incubated at room temperature for 24 h, and then heated at 8000 r Centrifuge at a speed of 1 / min, wash with methanol three times, and vacuum-dry at 50°C for 36 h to obtain ZIF-8. Mix 20 μL of 200 nM aptamer solution and 70 μL of 1.67 mg·mL -1 MOFs were incubated for 30 min to prepare functionalized MOFs, and the aptamer solution was ACGCGAATCGGAGTTGGGGGT.

[0062] (2) Construction of a colorimetric sensor based on aptamer-enhanced enzymatic activity of MOFs:

[0063] Prepare a series of standard solutions of fipronil, the concentrations are 0.1 μM, 0.2 μM, 0.4 μM, 0.5 μM, 0.8 μM, 1 μM, 2 μM, 3 μM, 4 μM, 5 μM. 15 μL of fipronil standard solutions of different concentrations ...

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Abstract

The invention provides a colorimetric sensor for enhancing MOFs enzyme-like activity based on an aptamer as well as a preparation method and application of the colorimetric sensor, and belongs to the technical field of sensor preparation and fruit and vegetable quality detection. According to the present invention, the colorimetric sensor is constructed by using the metal organic framework material (MOFs) with peroxidase-like activity to catalyze and oxidize 3, 3', 5, 5'-Tetramethylbenzidine (TMB), and the colorimetric sensor can achieve the visual rapid monitoring of the pesticide residues in the fruits and the vegetables based on the enhancement effect of the aptamer on the catalytic activity of the MOFs and the specific recognition between the aptamer and the pesticide.

Description

technical field [0001] The invention belongs to the technical field of sensor preparation and fruit and vegetable quality detection, and specifically relates to a colorimetric sensor based on an aptamer-enhanced MOFs enzyme activity, a preparation method and application thereof. Background technique [0002] The wide application of pesticides plays a positive role in promoting agricultural production. However, pesticides have disadvantages such as refractory degradation and strong toxicity, and are easily enriched in water, soil, fruits and vegetables. Therefore, it is necessary to detect pesticide residues. Traditional detection methods for pesticide residues include high-performance liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS / MS), etc. These methods are difficult to achieve rapid on-site detection due to expensive equipment and high operating costs. [0003] At present, in order to realize the rapid detection of pesticide residues, r...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 张新爱皇雪月石吉勇邹小波黄晓玮李志华张迪申婷婷
Owner JIANGSU UNIV
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