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Method for detecting coumarin series compounds

A technology for coumarin and compounds, which is applied in the field of simultaneous detection of multiple coumarin-based compounds, can solve the problems such as the detection sensitivity and analysis throughput need to be further improved, and achieves high precision and detection sensitivity and high analytical throughput. high volume effect

Inactive Publication Date: 2010-06-30
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The shortcomings of the above detection methods are that the detection sensitivity and analysis throughput still need to be further improved

Method used

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  • Method for detecting coumarin series compounds
  • Method for detecting coumarin series compounds
  • Method for detecting coumarin series compounds

Examples

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Effect test

Embodiment 1

[0049] This example is used to illustrate the method for detecting coumarin compounds according to the present invention.

[0050] (1) Preparation of coumarin compound standard solution

[0051] Accurately weigh an appropriate amount of each coumarin-based compound standard, dissolve it in methanol and dilute to 100 mL, prepare a 1000 mg / L standard stock solution, and store it at -20°C. Accurately measure 4 mL of each coumarin-based compound standard stock solution into a 100 mL volumetric flask, dilute to 100 mL with methanol, and prepare a 40 mg / L mixed standard stock solution. When in use, dilute it step by step with methanol to a mixed standard working solution with a concentration of 1, 2, 5, 10, 20, 50, 100, 200 and 250 ng / mL.

[0052]Wherein, the coumarin series compound standard product is: acenocoumarol (purity 98% by weight) purchased from Canada TRC company; dicoumarin (purity 99% by weight) and 8-methoxy psoralen (purity 99% by weight) purchased from Belgium Acro...

Embodiment 2

[0081] This example is used to illustrate the method for detecting coumarin compounds according to the present invention.

[0082] Accurately weigh 0.500g of a cosmetic sample (cream) into a 25mL stoppered colorimetric tube, add 10mL of acetonitrile, vortex and mix, ultrasonically extract for 20 minutes, centrifuge at 15,000 rpm for 15 minutes, and collect the supernatant The solution was concentrated in a 50mL chicken heart bottle with a rotary evaporator in a water bath at 40°C until it was nearly dry. After slowly blowing dry with nitrogen, 2mL of 10% by weight methanol aqueous solution was accurately added to dissolve the residue, and the obtained solution was used for solid phase extraction. After the Oasis HLB solid-phase extraction column is activated with 5mL of methanol and equilibrated with 5mL of water, the sample solution is passed through the column, and the direct effluent is discarded. For elution, collect the eluate of 90% by weight methanol aqueous solution, b...

Embodiment 3

[0084] This example is used to illustrate the method for detecting coumarin compounds according to the present invention.

[0085] Accurately weigh 0.500g of a cosmetic sample (for lipstick) into a 25mL stoppered colorimetric tube, add 2mL of tetrahydrofuran, ultrasonically extract for 5 minutes, add 8mL of methanol, vortex and mix, after ultrasonically extract for 20 minutes, at 15000 rpm Centrifuge at a speed of 10 minutes for 15 minutes, collect the supernatant in a 50mL heart-shaped bottle, concentrate to nearly dryness in a water bath at 40°C with a rotary evaporator, blow dry slowly with nitrogen, and accurately add 2mL of 10% by volume methanol aqueous solution to dissolve the residue. The resulting solution was used for solid phase extraction. After the OasisHLB solid-phase extraction column was activated with 5mL of methanol and equilibrated with 5mL of water, the sample solution was passed through the column, the direct effluent was discarded, rinsed with 1mL of 10vo...

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Abstract

The invention provides a method for detecting coumarin series compounds, which comprises the following steps that: ultra high-efficiency liquid chromatography and tandem mass spectrometry detection are carried out to a sample, so as to determine the types and the content of coumarin series compounds contained in the sample. The coumarin series compounds comprise one or more types in vinegar Glass coumarin, dicoumarol, 6-methoxy-7-hydroxy coumarin, 7-hydroxycoumarin, 8-hydroxy-psoralen, 4-methyl-7-hydroxy coumarin, 6,7-dimethoxy coumarin, coumarin, 7-methoxy coumarin, dihydro coumarin, psoralen, psoralen, 8-methoxypsoralen, 7-methyl, 5-methoxypsoralen, 4-methyl-7-ethoxyl coumarin, 4-methyl-7-diethylamino coumarin, 2', 4,8-trimethyl psoralen, imperatorin, isoimperatorin, columbianadin and cyclocoumarol. The method of the invention can complete the analysis and the detection of 22 coumarin series compounds within 4 min.

Description

technical field [0001] The invention relates to a method for detecting coumarin compounds, in particular to a method for simultaneously detecting multiple coumarin compounds. Background technique [0002] Coumarin-based compounds are a class of natural products with an aromatic odor and a benzoα-pyrone structure as the core. They are widely distributed in the plant kingdom and can be divided into simple coumarin-based compounds and furanocoumarin-based compounds according to their structures. Compounds, pyranocoumarin-based compounds and other coumarin-based compounds, etc. Cosmetics used in daily life usually add a certain amount of spices and essences to give cosmetics a warm and elegant aroma, but at the same time, the safety of added spices and essences will directly affect the safety of cosmetics. Coumarin-based compounds are widely used in cosmetics such as perfumes, shower gels and deodorants. According to the research results of the German Federal Institute for Ris...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/72
Inventor 马强白桦王超张庆董益阳肖海清闫妍杨海峰李坤威
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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