Green synthesis method of germanium quantum dot in solution, germanium quantum dot and application thereof in biological imaging
A green synthesis and quantum dot technology, applied in the field of nanomaterials, can solve the problems of limited application, high toxicity, and low fluorescence quantum yield in the field of optical and optoelectronic devices
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Embodiment 1
[0084] Synthesize water-soluble germanium quantum dots as follows:
[0085] (1) Weigh 0.06g of sodium hydroxide and dissolve it in 10ml of water, and ultrasonically dissolve it to make a sodium hydroxide solution;
[0086] (2) Weigh 0.26g germanium dioxide (GeO 2 , purchased from Sigma-Aldrich Shanghai Trading Co., Ltd., product number 483702) was dissolved in the sodium hydroxide solution in step (1), and ultrasonically dissolved to obtain a germanium source solution;
[0087] (3) Dissolving the germanium source in a sodium hydroxide solution under ultrasonic radiation conditions to prepare a germanium source solution, and adjusting the pH value of the germanium source solution in step (2) to between 6 and 7 with dilute hydrochloric acid;
[0088] (4) Take 1ml of the germanium source solution in step (3) and add it to 7ml of water;
[0089] (5) Magnetic stirring at a speed of 1000r / min. Add 0.0250g of polyvinylpyrrolidone (PVP, purchased from Bailingwei Technology, 968832) ...
Embodiment 2
[0112] The germanium quantum dot obtained in embodiment 1 is used for cytotoxicity experiment:
[0113] Lung cancer cells (A549 cell line, purchased from ATCC, number CCL-185) were separated into 10 cells per 35 mm inner diameter of the culture dish. 3 The density of each was inoculated in a petri dish containing 2ml of DMEM medium (purchased from Gibco, brand sh30022.01B, and containing 10vol% fetal bovine serum), at 37°C and 5vol% CO 2 After cultivating at a concentration of 24 h, the culture medium was aspirated, and buffer was added, and the germanium quantum dots The level of apoptosis was detected in the presence of the cells.
[0114] The specific operation steps are as follows:
[0115] (1) Prepare 100 μL of cell suspension in a 64-well plate, and pre-culture the culture plate in an incubator for 24 hours (37°C, CO 2 5vol%);
[0116] (2) Add 10 μL of different concentrations of germanium quantum dots to the culture plate so that the concentrations of germanium quan...
Embodiment 3
[0123] The germanium quantum dot obtained in embodiment 1 is used for cell imaging test:
[0124] Lung cancer cells (A549 cell line, purchased from ATCC, number CCL-185) were separated into 10 cells per 35 mm inner diameter of the culture dish. 3 The density of each was inoculated in a petri dish containing 2ml of DMEM medium (purchased from Gibco, brand sh30022.01B) and containing 10% by volume of fetal bovine serum), at 37°C and 5vol% of CO 2 After culturing for 24 hours at the concentration, the medium was sucked out, and 2ml of the medium containing germanium quantum dots (the drug concentration was 2mg / ml) was added to the petri dish, and the petri dish was kept at 37°C and 5vol% CO 2 Incubate for 4 hours at the same concentration, aspirate the medium, wash with phosphate buffer (purchased from Gibco, brand P1020-500) 3 times (5ml each time), and use a fluorescence microscope to image.
[0125] Imaging results such as Figure 6 As shown, it can be seen that after the ge...
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