119 results about "Fluorescence immunoassay analyzer" patented technology

The invention relates to the field of immuno-chromatographic detection, in particular to a quantitative detection calculation method based on fluorescent immuno-chromatographic technology. The method includes the steps: inserting a fluorescent immuno-chromatographic test strip dripping sample solution to be measured into a fluorescent immunity analyzer; irradiating by the aid of LED (light-emitting diode) light sources; performing light filtering treatment by the aid of a light filter; detecting by the aid of a photoelectric detector; treating electric signals; performing AD conversion treatment; performing filtering algorithm treatment; performing baseline fitting; performing peak searching treatment; calculating T / C area ratio; calculating concentration; judging by the aid of calculated concentration results. The invention provides a perfect, rapid and quantitative detection calculation method based on the fluorescent immuno-chromatographic technologies. Various noise and signal interference can be filtered when concentration of objects to be measured is detected and calculated, and accurate detection results are improved. The method has the advantages of high accuracy and stability and low power consumption, and rapid and quantitative detection requirements of users are effectively met.

The invention discloses a full-automatic fluorescence immunoassay analyzer and a sample detection method, the analyzer comprises a bracket, the bracket is arranged on a base, a sample feeding and discharging device is arranged on the base, and the sample feeding and discharging device is used for pushing a sample tube into a sample feeding device for detection and pushing the sample tube out for sample discharging; a sample tube uniform mixing assembly is arranged on the side surface of the sample feeding and discharging device and is used for uniformly mixing samples in a sample tube, and thesample tube is pushed into the sample feeding and discharging device to be detected and pushed out to be discharged; the analyzer also comprises a sampling needle moving assembly which is used for extracting a sample in the sample tube; a reagent bin mechanism is arranged in the bracket, a plurality of reagent cards are stacked in the reagent bin mechanism, and diluted samples are added into sample adding ports of the reagent cards by the sampling needle; and a light path detection assembly for detecting the reagent card is also arranged in the bracket. The analyzer is reasonable and compactin overall layout, complete in function, capable of achieving full-automatic operation, user-friendly in design, efficient and rapid.

The invention discloses an automatic card return mechanism and a single-channel fluorescence immunoassay analyzer. The automatic card return mechanism comprises a bottom plate, a sliding rail, a sliding mechanism, a position retaining mechanism and a card pushing mechanism, an elongated hollow portion is formed on the bottom plate, a card return slideway is fixed below the bottom plate, a slidewayport for a testing card to go in and out is arranged at a first end of the card return slideway, and the sliding rail is mounted on the bottom plate and is close to the hollow portion; the sliding mechanism is movably mounted on the sliding rail and can move along the same, the testing card can be put on the sliding mechanism, the position retaining mechanism is fixed on the bottom plate and is close to a second end of the card return slideway, and the card pushing mechanism is mounted on the sliding mechanism and partly extends into the card return slideway and can push the testing card outof the slideway port of the card return slideway to realize automatic card return. The automatic card return mechanism is simple in structure, capable of realizing active card pushing, low in card return fault, high in stability and capable of improving testing efficiency.

The invention discloses a portable fluorescence immunity analyzer. The portable fluorescence immunity analyzer comprises a test strip bin, an excitation light source, a camera, a communication moduleand a processing module, wherein the test strip bin is used for being inserted with a fluorescence chromatography rapid diagnosis test strip; the excitation light source is arranged right above a color developing region of the fluorescence chromatography rapid diagnosis test strip and used for exciting a fluorescent substance on the fluorescence chromatography rapid diagnosis test strip to producefluorescence emitting lights; the camera is arranged at an entrance above an acquisition channel on the excitation light source and used for acquiring the fluorescence emitting lights of a fluorescence test strip; the communication module is used for sending an image acquired by the camera; and the processing module is used for controlling the excitation light source, the camera and the communication to work harmoniously.

The invention belongs to the field of immuno-chromatographic detection, and in particular relates to a quantitative detection and computation method based on the fluorescence immuno-chromatographic technology, comprising the following steps: inserting a fluorescence immunostrip dripped with a sample solution to be detected into a fluorescence immunoassay instrument, and sequentially performing irradiation with an LED light source, optical filtering processing with an optical filter, detection with a photoelectric detector, electrical signal processing, AD conversion processing, baseline correction, L-M algorithm processing, area computation and concentration computation. The quantitative detection and computation method stresses on signal reconstruction by using an algorithm, eliminates influence of noise and various interferences, ensures the signal processing course undistorted, and can obtain high-precision and low-error results, thereby quickly, conveniently and accurately determining the concentration result.

The invention discloses a novel coronavirus (COVID-19) neutralizing antibody detection kit, which is characterized by comprising: a detection card. The detection card comprises a card shell and a test strip arranged in the card shell, the test strip comprises a sample pad, a fluorescence combination pad, a nitrocellulose membrane, a water absorption pad and a backing plate. The fluorescent combination pad comprises a detection line fluorescent conjugate and a quality control line fluorescent conjugate, the detection line fluorescent conjugate is a novel recombinant coronavirus spinous process glycoprotein (RBD) marked by fluorescent microspheres, and the nitrocellulose membrane is coated with a detection line formed by angiotensin converting enzyme 2 (ACE2). And the nitrocellulose membrane is also coated with a quality control line formed by IgY, IgG or DNP antibodies. The kit is combined with a fluorescence immunoassay analyzer to detect a sample, and the kit is high in detection sensitivity, good in reproducibility, high in stability and convenient to operate.

The invention discloses a fluorescent standard card for calibration and quality control of a fluorescent immunoassay analyzer. The fluorescent standard card comprises a fluorescent standard card bodyand a fluorescent detecting unit. The fluorescent detecting unit is made of a fluorescent solution, a photocurable fluorescent material, fluorescent printing ink or fluorescent ink. Compared with theuse of a quality control product, reference product or standard product, the fluorescent standard card has the characteristics of stable luminescent properties, being not easy to decay with time, highline width precision, uniform spatial distribution of fluorescent substances, linearity of fluorescent substance amount and fluorescent intensity and great preparation consistency. The invention further discloses a test method. The method and parameters of a fluorescent standard card evaluation device are established. The method has the advantages of complete evaluation content and easy operation, is a systematic measurement and evaluation scheme, is beneficial for calibration and quality control of the fluorescent immunoassay analyzer, and improves the detection accuracy of the fluorescent immunoassay analyzer. The traceability scheme of each method can reliably ensure that the properties of the fluorescent standard card are strictly controlled during production and use.

The invention discloses a medical turntable type multi-project fluorescent immunization analyzer, which comprises an automatic casing, a display screen, a thermal-sensitive printer, a power switch button, a card slot, an automatic card feeding assembly, an electric turntable assembly, a barcode recognition assembly, and a detection and analysis assembly. The automatic card feeding assembly includes a fixed bracket, a screw motor, a clamp slot connecting plate, a clamp slot and a micro switch. A semiconductor laser is used for emitting laser with the specific wavelength to irradiate a sample and fluorescence with the certain wavelength is excited; the fluorescent light intensity is received by a photosensitive diode and an optical signal is converted into a voltage signal; and the concentration value of the measured object is negatively correlated with the fluorescent light intensity. Therefore, the concentration and content of the measured object are measured. The batch detection and multi-item detection are realized; the labor intensity of the testing personnel is reduced; the requirements of high efficiency and accuracy of detection are met.

The invention discloses a full-automatic magnetic-bead time resolution fluorescence immunoassay analyzer. The analyzer is divided into an inner chamber and an outer chamber by a rack; an electronic element box is fixed on the upper layer of the inner chamber, a dark chamber shell is positioned on the lower layer of the inner chamber, a dark chamber door device is arranged on the rack, and a dark chamber space of which one side is openable is formed by the dark chamber door device and the dark chamber shell; a tray module is positioned on the lower layer of the outer chamber, and a plurality ofreagent strips can be placed on the tray module, and are conveyed to the lower layer of the inner chamber or are withdrawn to the lower layer of the outer chamber; a puncturing liquid relief module is positioned on the upper layer of the outer chamber, can automatically pierce the film of the top surface of each reagent strip, and automatically suck or discharge liquid in the reagent; a scanningand value reading module is positioned on the lower layer of the inner chamber, is positioned in the dark chamber shell, and is used for triggering and reading fluorescence information of the reagentstrips after incubation; and by reasonable arrangement of the inner chamber and the outer chamber and in combination with compact and scientific design of the related functional module, the structureis simple and compact, the cost is low, furthermore, the degree of automation of the integral instrument is increased, and thus, the full-automatic magnetic-bead time resolution fluorescence immunoassay analyzer is quite suitable for being used and popularized in China medical units.

The embodiment of the invention relates to the field of immunochromatography detection, and discloses a method for detecting the concentration of a substance to be detected, a fluorescence immunoassayanalyzer and a storage medium, and the method comprises the steps: carrying out the first image processing of a fluorescence image, so as to remove non-fluorescence regions at two sides of the chromatography direction of the fluorescence image, and obtaining a first fluorescence image; and on the basis of the first fluorescence image, removing the non-fluorescence regions on the two sides of thevertical chromatography direction to obtain a second fluorescence image, so that the interference of the non-fluorescence regions on the two sides of the vertical chromatography direction on subsequent image analysis and calculation can be avoided, namely, the interference on subsequent concentration calculation is reduced. Namely, the non-fluorescent region is removed step by step twice, so thatthe fluorescent region can be accurately positioned, and the concentration detection accuracy is high.

The invention discloses a medical hand-held fluorescent immunoassay analyzer, which comprises an upper shell, a lower shell, a smart phone, a focusing lens, a battery, a fluorescent module, a motor control board, a micro screw motor, a first microswitch and a second microswitch. A camera arranged at the back of the smart phone is screwed to the focus lens and is used for scanning a barcode of a reagent card. The upper shell is arranged at the top of the upper end of the fluorescent immunoassay analyzer. The battery is mounted on the inner side of the lower shell. The micro screw motor and thefluorescent module are mounted on the back side of the motor control board. With the medical hand-held fluorescent immunoassay analyzer, batch detection and multi-item detection are realized; the labor intensity of the testing personnel is reduced; the requirements of high efficiency and accuracy of detection are met. The medical hand-held fluorescent immunoassay analyzer has advantages of havinga small size, being convenient to carry, being convenient to realize field work by the testing personnel, and being used flexibly.

The invention relates to the technical field of detection, and discloses a fluorescence immunoassay analyzer, which comprises an incubation disc structure, and a photographing recognition mechanism and a scanning kicking mechanism which are positioned above the incubation disc. Rapid and high-throughput detection is realized by using a compact and simple instrument structure, the analyzer is suitable for various detection scenes, and has extremely high application value.

The invention relates to the technical field of fluorescence immunoassay and particularly relates to a method for calibrating inter-platform difference of a fluorescence immunoassay analyzer. The method comprises steps of performing logarithm operation processing on n pairs of arrays obtained by testing of a testing machine and a standard machine, then performing curve fitting to obtain a corresponding fitting curve function, substituting actual measurement data logarithm operation processing into the fitting curve function to obtain a corresponding value during actual testing, and finally performing power multiplication calculation to obtain a calibrated numerical value. By means of the calibration method, no matter when the fluorescence signal intensity value is low or when the fluorescence signal intensity value is high, deviation of the obtained calibrated value is within 3%, and calibration deviation is extremely low; the method can be well suitable for common low-value interval fluorescence immunoassay indexes, is wide in application range, enables a final detection result to be more accurate, improves the detection accuracy of an instrument, and effectively solves a problemthat a conventional calibration method is too large in correction deviation when the intensity value of a fluorescence signal is low.

The invention relates to the field of immunochromatography in medical immunology, and in particular to a fluorescent immunochromatographic detection kit for determining ST2. The fluorescent immunochromatographic detection kit is composed of an immunochromatographic test strip and a plastic outer shell, and the immunochromatographic test strip is a test strip composed of a bottom plate, a sample pad, a reagent pad, a nitrocellulose membrane and an absorbent pad. the sample pad, the reagent pad, the nitrocellulose membrane and the absorbent pad are sequentially staggered and fixed on the bottomplate, the reagent pad is coated with colored fluorescent microspheres labeled with a mouse anti-human ST2 monoclonal antibody, a detection line on the nitrocellulose membrane is coated with a rabbitanti-human ST2 polyclonal antibody, a control line is coated with a goat anti-mouse polyclonal antibody, the kit has a qualitative determination boundary concentration of 40-55 ng / ml, a fluorescence immunoassay analyzer has a detection sensitivity of 1 ng / ml and a linear range of 1 to 400 ng / ml. The color fluorescent microspheres can quickly obtain qualitative results and obtain quantitative results later. The kit has the characteristics of simple operation, quick response and high sensitivity, and is helpful for satisfying the requirement of different examination scenes.

The invention discloses an automatic pipeline for blood cell analysis and POCT immunoassay, and the pipeline comprises a rack, a first conveying platform, a first mixing device, a first sampling mechanism, a first detector with an analyzing device, a first unloading platform, a first conveying device, a second conveying platform, a relay mechanism, a second mixing device, a second sampling mechanism and a fluorescent immunoassay analyzer with a plurality of test channels, wherein the automatic pipeline for blood cell analysis and POCT immunoassay with the above structure organically combines blood cell analysis and fluorescence immunoassay to achieve detection of blood cells and POCT immunization programs, and the plurality of test channels using the fluorescent immunoassay analyzer can freely combine different test items, thereby providing versatility and expandability, shortening sample detection cycle, improving efficiency, reducing manual intervention and reducing error rates.

The invention relates to a fluorescence immunoassay analyzer and a reagent card inserting, guiding and anti-pulling mechanism, and belongs to the field of immunoassay instruments. The fluorescence immunoassay analyzer comprises a support, and a reagent card inserting groove seat, an information reading head, a linear displacement output device and a reagent card inserting guide anti-pulling mechanism which are arranged on the support; the reagent card inserting, guiding and anti-pulling mechanism is arranged beside the inlet where the reagent card is inserted into the containing groove and comprises a roller mounting base, a guiding roller, a one-way rotation locking mechanism and an unlocking mechanism; the guide roller is rotatably mounted on the roller mounting seat and is at least usedfor providing guidance for the inserting action of the reagent card; the one-way rotation locking mechanism is used for releasably locking one-way rotation of the guide roller towards the reverse direction of the plug-in card; and the unlocking mechanism is used for controlling the working state of the one-way rotating locking mechanism so as to release the steering locking state of the guide roller. Based on the additionally-arranged reagent card inserting guiding anti-pulling mechanism, the risk can be effectively reduced, and the fluorescence immunoassay analyzer can be widely applied to the rapid detection of new coronaviruses and the like.

The invention discloses a time-resolved fluorescence immunoassay analyzer which comprises a base, and a sample injection module, an incubation module, a cleaning module and a chip detection module. Achip loading module and a liquid path and gas path module are arranged in the sample injection module, wherein the chip loading module is used for carrying a chip to the liquid path and gas path module, and the liquid path and gas path module is used for enabling a sample in the chip to flow to a preset position, and then, the chip is transferred into the incubation module; the incubation module enables the chip to achieve thermostatic reaction within a set temperature; the incubation module freely moves up and down along the vertical direction; the sample injection module, the cleaning moduleand the chip detection module are respectively arranged on the side surface of the incubation module in the vertical movement direction; and the incubation module sends the chips cleaned in the cleaning module to the chip detection module for detection. All modules are arranged in the vertical direction, so that the overall structure is compact, and the occupied area is small; and each module issmooth and simple in function cooperation and detection efficiency is high.

The invention relates to an automatic information recognition method of a fluorescent immune analyzer. The method comprises the following steps: setting a two-dimensional code containing test parameter information on the surface of a test card, and setting a camera module in the fluorescence immune analyzer; after the test card is put into the fluorescent immune analyzer, scanning the two-dimensional code of the test card by the camera module to obtain the test parameter information, and then performing data processing by the fluorescence immune analyzer through the obtained test parameter information to form a standard curve of the fluorescent immune analyzer; recognizing a sample number in a sample number handwriting area on the test card by the camera module, and inputting the sample number information into the fluorescence immune analyzer to match the test parameter information and a measurement result. By adopting the method, the situation of information misalignment caused by themanual operation error in the fluorescence immune analyzer is avoided, multi-sample detection is facilitated, the manual operation intensity is reduced and the detection efficiency is improved.

The invention discloses a semi-automatic dry type fluorescence immunoassay analyzer. The semi-automatic dry type fluorescence immunoassay analyzer comprises a card inlet mechanism, a card outlet mechanism, a system shell, code scanning devices, a measurement mechanism and a control board; the code scanning devices comprise the first code scanning device and the second code scanning device, and the card inlet mechanism, the card outlet mechanism and the measurement mechanism are installed on the system shell; the card inlet mechanism is provided with a first photoelectric sensor and a second photoelectric sensor; the card inlet mechanism is further provided with a card inlet and a card outlet, the first photoelectric sensor is arranged at the card inlet, and the second photoelectric sensor is arranged at the card outlet; the first code scanning device, the second code scanning device, the first photoelectric sensor, the second photoelectric sensor, the card outlet mechanism and the measurement mechanism are electrically connected with the control board. The semi-automatic dry type fluorescence immunoassay analyzer can solve the problems that reagent cards are blocked when entering and conveying are carried out and incompatible when read, and measurement is inaccurate and exceeds time.

The invention relates to a dry fluorescence immunity analyzer which comprises a casing, wherein one side, which faces a user, of the casing is defined as a front side, and a direction of the front side is defined as front; the dry fluorescence immunity analyzer is characterized by comprising a sample injection drive part, a transfer rotating disc, a mechanical pusher, a photoelectric detection unit and a material recovery port; the sample injection drive part is arranged on the front side of the casing; the transfer rotating disc is arranged in the casing; the mechanical pusher is arranged inthe casing and controlled by a control unit; the photoelectric detection unit is positioned on the left side or the right side of the transfer rotating disc; and the material recovery port is formed in the front side of the casing. According to the dry fluorescence immunity analyzer provided by the invention, multiple self-inspection cards can be fed at one time, samples on each self-inspection card can be sequentially detected by the photoelectric detection unit, the detection efficiency is greatly improved, and the detection time is saved.

The invention provides a high-flux photographic fluorescence immunoassay instrument, comprising a bottom plate; a tray for holding test strips is arranged above the bottom plate; the tray is connectedwith a driving device that drives the tray to rotate; the tray is provided with a plurality of receiving grooves for holding test strips, the receiving grooves are evenly distributed around the axisof the tray, the middle of the tray is provided with a push-out mechanism that corresponds to the receiving grooves and functions to push out the test strips, and a scanning device and a detecting device connected with a controller are arranged above the receiving grooves. The high-flux photographic fluorescence immunoassay instrument is capable of detecting multiple items in the same time, addingthe items according to durations is not required, the scanning device has auto-timing and auto-detecting functions, and the high-flux photographic fluorescence immunoassay instrument is convenient tooperate and has greatly improved detection efficiency.

Disclosed are an automated card withdrawal mechanism and a single-channel fluorescent immunoassay instrument. The automated card withdrawal mechanism comprises a base plate (10), a slide rail (20), a sliding mechanism (30), a position blocking mechanism (40) and a card pushing mechanism (50), wherein the base plate (10) is provided with a long strip-shaped hollowed part (11), a card withdrawal slideway (12) is fixed under the base plate (10), a first end of the card withdrawal slideway (12) is provided with a slideway entrance enabling a test card (70) to go in and out; the slide rail (20) is mounted on the base plate (10) and is close to the hollowed part (11); the sliding mechanism (30) is movably mounted on the slide rail (20) and can move in the direction of the slide rail (20), and the test card (70) can be placed on the sliding mechanism (30); the position blocking mechanism (40) is fixed on the base plate (10) and at a position near a second end of the card withdrawal slideway (12); and the card pushing mechanism (50) is mounted on the sliding mechanism (30) and partially extends into the card withdrawal slideway (12), and can push the test card (70) out of the slideway entrance of the card withdrawal slideway (12), realising automated card withdrawal. The mechanism can realise active card pushing with reduced card withdrawal failure and high stability, and testing efficiency can be improved.

The invention discloses a full-automatic fluorescence immunoassay analyzer and a sample detection method. The analyzer comprises a support, the support is arranged on a base, a sample feeding and discharging device is arranged on the base, a sample tube mixing assembly is arranged on the side face of the sample feeding and discharging device, and a sample tube is fed and discharged through the sample feeding and discharging device; the analyzer further comprises a sampling needle and dilution needle module which is used for extracting a sample, adding the sample into a uniform mixing reaction tank and adding a diluent into the uniform mixing reaction tank for diluting and uniformly mixing, and the sampling needle is used for adding the diluted and uniformly mixed sample into a reagent card; a reagent card bin assembly is arranged in the support, a plurality of reagent card boxes are placed in the reagent card bin assembly, and the reagent card is conveyed through a reagent card carrying assembly; a detection assembly for detecting the reagent card is further arranged in the support; the analyzer further comprises a waste reagent card disposal shifting device which is used for shifting the reagent card after detection to a waste receiving barrel. The analyzer is reasonable in overall layout, complete in function and capable of achieving full-automatic operation.

The invention provides a myoglobin time-resolved fluorescence immunochromatography testing kit. The kit comprises a detection card and a fluorescent microsphere working fluid. The detection card comprises the detection card used for detecting myoglobin. The fluorescent microsphere working fluid contains a time-resolved fluorescence microsphere for marking a corresponding antibody. During a detection process, fluorescent microsphere compounds in a detection area and a quality control area are combined, fluorescence emitted under an effect of excitation light is collected and processed by a fluorescence immunoanalyzer, and then a detection result can be calculated. The kit has advantages that operation is simple; measurement is accurate; sensitivity is high; and specificity is high.

The invention relates to a small full-automatic time-resolved fluorescence immunity analyzer. The small full-automatic time-resolved fluorescence immunity analyzer is characterized in that a base of aZ-axis lead screw module is fixed on a slider of an X-axis lead screw, an injection pump is fixed on the slider of a Z-axis lead screw, and a spearhead can be inserted into an output port of the injection pump; a spearhead withdrawing mechanism is fixed on one side of the injection pump, a Y-axis lead screw is connected with a support accommodating a kit, a micro centrifuge module and a support plate are also mounted on the support, a reaction plate is placed on the support plate, a linear slide track is mounted on each of two sides below the support plate, the bottom surfaces of the linear slide tracks are fixed on the support, one lead screw motor module is connected to each of two sides of the support plate to control the support plate to enter into or withdraw from a measurement darkchamber on a rack, a signal wire of a fluorescence detection module in the dark chamber is connected with a wireless data transmission module, and the wireless data transmission module can communicated with a smartphone and can transmit measurement data and a control instruction; the small full-automatic time-resolved fluorescence immunity analyzer is applicable to families, community public health stations or sanitary mobile detection vehicles and hospital POCT (Point-of-care Testing).

The invention provides a time-resolved fluorescence immunochromatographic assay kit for hepatitis B5 tests. The time-resolved fluorescence immunochromatographic assay kit comprises detecting cards andfluorescent microsphere working fluid; the detecting cards comprise the detecting card used for detecting HBsAg, the detecting card used for detecting HBeAg, the detecting card used for detecting anti-HBs, the detecting card used for detecting anti-HBe, and the detecting card used for detecting anti-HBc; and the fluorescent microsphere working fluid contains time-resolved fluorescent microspheresfor marking the corresponding antigen or the corresponding antibody. In the detecting process, by combining fluorescent microsphere compounds in a detecting area and a quality control area, under theeffect of exciting light, emitted fluorescence is collected and processed by a fluorescence immunoassay analyzer, and thus the detecting result can be calculated. The time-resolved fluorescence immunochromatographic assay kit has the advantages of high sensitivity, high specificity, easy and convenient operation and accurate measuring.

The invention discloses a time-resolved fluorescence immunoassay kit for detecting novel coronavirus and a preparation method thereof. The kit comprises a test strip and a fluorescence immunoassay analyzer. The test strip comprises a bottom plate, and a sample filter pad, a microsphere marking pad, a detection pad and a water absorption pad which are located on the bottom plate. One end of the detection pad is laminated with one end of the microsphere marking pad, the other end of the detection pad is laminated with one end of the absorbent paper, and one end of the sample filter pad is laminated with the other end of the microsphere marking pad. Qualitative and quantitative detection of the novel coronavirus COVID-19 in various human body samples can be realized, and aiming at different application scenes and requirements, small-batch and easy-to-operate rapid early screening by primary communities and high-throughput and high-accuracy clinical diagnosis by a central hospital can be met at the same time.

The invention discloses a time-resolved fluorescent immunochromatographic card for detecting vomitoxin and acetylated derivatives thereof, and belongs to the fields of immunochromatography and food safety detection. The chromatographic card is composed of a sample pad, a labeling pad, a nitrocellulose membrane, water absorbing filter paper and a PVC bottom plate. The labeling pad is fixed with a nano europium fluorescent microsphere-labeled vomitoxin monoclonal antibody 1 and antibody 2. The antibody 1 is highly specific to vomitoxin and 15-acetyl-deoxynivalenol; the antibody 2 is highly specific to vomitoxin and 3-acetyl-deoxynivalenol. The chromatographic card is used for detection based on an antigen antibody immunology principle. The chromatographic card is combined with a portable time-resolved fluorescence immunoassay instrument, is used for rapid quantitative determination of vomitin and the acetylated derivatives thereof in cereals and products thereof, and has high sensitivityand good stability; the detection limit is 0.168 mg / kg, the detection range is 0.17-5 mg / kg, the time is 10-15 min, the operation is easy, professional training is not needed, and the chromatographiccard is widely applicable to needs for various occasions and different levels of personnel .

The invention relates to the field of medical instruments, in particular to an automatic dry type fluorescence immunoassay analyzer. The automatic dry type fluorescence immunoassay analyzer is provided with a rack, a card supply mechanism, a conveying mechanism, a sample adding mechanism and a plurality of reagent bottles, wherein the card supply mechanism, the conveying mechanism, the sample adding mechanism and the reagent bottles are arranged on the rack. A plurality of storage units arranged in parallel are arranged on a bearing frame, the bearing frame can move relative to the rack so that the corresponding storage units can be aligned with card supply openings of the rack, it is ensured that the mechanism can provide corresponding reagent cards according to analysis items, and the situation that the reagent cards are supplied disorderly is avoided. Each storage unit is internally provided with a plurality of reagent cards which are stacked up and down, it is ensured that the conveying mechanism can obtain sufficient reagent cards, the card supply mechanism inputs the reagent cards into storage grooves in a rotary disc from card inlets, the multiple storage grooves are arranged around the circumference of the rotary disc, and the multiple reagent cards can be subjected to test analysis at the same time. A sample adding driving device can drive a sample adding pipe so thatreagents in the reagent bottles can be automatically transferred to the reagent cards on the rotary disc, the test analysis efficiency is improved, and manual intervention is not needed.